| Summary: | 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is toxic contaminant which persistent in the environment. This compound bio accumulate through food chain and generate adverse effect in human health. Oxidative stress due to TCDD exposure can interference metabolism process and leads to the development of hepatotoxicity. The aim of the current study was to explore the role of casein derived from goat milk yogurt to prevent oxidative stress generate by TCDD intoxication. The study included 24 Wistar rats divided into 6 groups: (1) control group, (2) placebo group which given casein 600 mg/kg BW daily, (3) TCDD group dosed with TCDD 100 ng/kg BW diluted in corn oil daily, (4) group 1 given casein 300 mg/kg BW and TCDD 100 ng/kg BW daily, (5) group 2 given casein 600 mg/kg BW and TCDD 100 ng/kg BW daily, (6) group 3 given casein 900 mg/kg BW and TCDD 100 ng/kg BW daily. The respective dose were given orally for 21 days. Blood serum was collected for cholesterol, triglyceride, ALT and AST level. Liver collected for antioxidant enzyme superoxide dismutase (SOD) activity assay. Quantitative analysis using one way anova and post hoc Tukey test shows that casein derived from goat milk yogurt increased SOD activity in TCDD intoxicated rats. Significant increase (P<0.05) shows from group given with casein dose 600 and 900 mg/kg BW. Lipid profile shows normal value in all groups. There are no significant change in serum cholesterol and triglyceride level. Furthermore, there are no significant change in transaminase enzymes based on serum ALT and AST. Casein derived from goat milk yogurt has antioxidant activity and protective effect against oxidative stress induced by TCDD intoxication.
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