Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat
Melanocortin 4 Receptor (MC4R) is one of candidates gene for growth traits and feed intake in mammals. The objective of this research was to identify the genotype of Menocortin 4 Receptor gene in Bligon goat. Blood samples from 40 Bligon goats were used for DNA extraction. Primer used was designe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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UB Press
2016
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/274690/1/LATIFAH_APIS%202016%20NEW.pdf |
| _version_ | 1826049857495760896 |
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| author | Latifah, Latifah Priyadi, Dwi Ahmad Maharani, Dyah Kustantinah, Kustantinah Hartatik, Tety |
| author_facet | Latifah, Latifah Priyadi, Dwi Ahmad Maharani, Dyah Kustantinah, Kustantinah Hartatik, Tety |
| author_sort | Latifah, Latifah |
| collection | UGM |
| description | Melanocortin 4 Receptor (MC4R) is one of candidates gene for growth traits and feed
intake in mammals. The objective of this research was to identify the genotype of Menocortin
4 Receptor gene in Bligon goat. Blood samples from 40 Bligon goats were used for DNA
extraction. Primer used was designed from Capra hircus genome in Genbank Accession
Number NM_001285591. Primer CapraPF:5’-ATTTCCAAGTGATGCCGACC’3 and
CapraPR:5’- CTCCAACAAGCTGATGACCC-‘3 were used to amplify 387 bp of PCR
product. Using DNA sequencing, a Single Nucleotide Polymorphism (SNP) was detected in
Bligon goat. The SNP identified in this research was g.256 G/A. The results of DNA
sequence of PCR product in Bligon goats were used for restriction map of specific enzyme
using Bioedit version 7.2.0. Based on restriction mapping, HpyCH4V (A'CG_T) was detected
to recognize SNP region. Rectriction enzyme HpyCH4V can be usedfor genotyping of
targeted gene using PCR-RFLP method and it will be assosiated with growth traits and feed
intake in Bligon goat. |
| first_indexed | 2024-03-13T23:53:50Z |
| format | Article |
| id | oai:generic.eprints.org:274690 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:53:50Z |
| publishDate | 2016 |
| publisher | UB Press |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2746902018-10-24T00:55:45Z https://repository.ugm.ac.id/274690/ Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat Latifah, Latifah Priyadi, Dwi Ahmad Maharani, Dyah Kustantinah, Kustantinah Hartatik, Tety Animal Production Animal Breeding Melanocortin 4 Receptor (MC4R) is one of candidates gene for growth traits and feed intake in mammals. The objective of this research was to identify the genotype of Menocortin 4 Receptor gene in Bligon goat. Blood samples from 40 Bligon goats were used for DNA extraction. Primer used was designed from Capra hircus genome in Genbank Accession Number NM_001285591. Primer CapraPF:5’-ATTTCCAAGTGATGCCGACC’3 and CapraPR:5’- CTCCAACAAGCTGATGACCC-‘3 were used to amplify 387 bp of PCR product. Using DNA sequencing, a Single Nucleotide Polymorphism (SNP) was detected in Bligon goat. The SNP identified in this research was g.256 G/A. The results of DNA sequence of PCR product in Bligon goats were used for restriction map of specific enzyme using Bioedit version 7.2.0. Based on restriction mapping, HpyCH4V (A'CG_T) was detected to recognize SNP region. Rectriction enzyme HpyCH4V can be usedfor genotyping of targeted gene using PCR-RFLP method and it will be assosiated with growth traits and feed intake in Bligon goat. UB Press 2016 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/274690/1/LATIFAH_APIS%202016%20NEW.pdf Latifah, Latifah and Priyadi, Dwi Ahmad and Maharani, Dyah and Kustantinah, Kustantinah and Hartatik, Tety (2016) Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat. Proceeding 3rd Animal Production International Seminar (3rd APIS) & 3rd ASEAN Regional Conference on Animal Production (3rd ARCAP). ISSN 978-602-432-017-1 |
| spellingShingle | Animal Production Animal Breeding Latifah, Latifah Priyadi, Dwi Ahmad Maharani, Dyah Kustantinah, Kustantinah Hartatik, Tety Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat |
| title | Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat |
| title_full | Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat |
| title_fullStr | Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat |
| title_full_unstemmed | Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat |
| title_short | Identification of Single Nucleotide Polimorphism of Melanocortin 4 Receptor Gene in Bligon Goat |
| title_sort | identification of single nucleotide polimorphism of melanocortin 4 receptor gene in bligon goat |
| topic | Animal Production Animal Breeding |
| url | https://repository.ugm.ac.id/274690/1/LATIFAH_APIS%202016%20NEW.pdf |
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