Semi Purification and Identifications Molecule Protein Weigh of Alkaline Protease Enzyme from Bacillus cereus LS2B
Alkaline protease enzyme from Bacillus cereus LS2B was successively purified by three steps procedure including ammonium precipitation, membrane dialysis, and HiTrap ion exchange chromatography with DEAE Sepharose FF matrix. The best enzyme concentration was obtained by precipitation using 80% of a...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Science and Engineering Research Support soCiety
2017
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Subjects: | |
Online Access: | https://repository.ugm.ac.id/274733/1/Mix.pdf |