Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis)
Porcine contamination was one important issue in food certification for Halal. Here, we reported PCR combined with RFLP technique to detect porcine contamination in meatball. Various concentration of pork was added to beef in preparation of the meatball. Mithocondrial DNA was then isolated from the...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/275133/1/ICCS%20Tri%20Joko%20R.pdf |
| _version_ | 1797036924030943232 |
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| author | Raharjo, Tri Joko Sismindari, Sismindari |
| author_facet | Raharjo, Tri Joko Sismindari, Sismindari |
| author_sort | Raharjo, Tri Joko |
| collection | UGM |
| description | Porcine contamination was one important issue in food certification for Halal. Here, we reported PCR combined with RFLP technique to detect porcine contamination in meatball. Various concentration of pork was added to beef in preparation of the meatball. Mithocondrial DNA was then isolated from the meatball followed by PCR using published primers that amplified cytB gene. A + 350 bp DNA fragment was resulted from PCR. PCR product was targeted for digestion by means of BamHI and BseDI enzymes. Since these enzymes cut porcine cytB gene but does not cut beef cytB gene, all meatball contain porcine contamination give small DNA fragment (+ 220 and 130 bp) in electrophoresis analysis, while the absent of these DNA fragment leading to conclusion that meatball sample free from porcine. This method has been validated to detect up to 1% of porcine present in the meatball. |
| first_indexed | 2024-03-13T23:54:32Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:275133 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:54:32Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2751332019-05-22T00:53:50Z https://repository.ugm.ac.id/275133/ Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) Raharjo, Tri Joko Sismindari, Sismindari Analytical Chemistry not elsewhere classified Porcine contamination was one important issue in food certification for Halal. Here, we reported PCR combined with RFLP technique to detect porcine contamination in meatball. Various concentration of pork was added to beef in preparation of the meatball. Mithocondrial DNA was then isolated from the meatball followed by PCR using published primers that amplified cytB gene. A + 350 bp DNA fragment was resulted from PCR. PCR product was targeted for digestion by means of BamHI and BseDI enzymes. Since these enzymes cut porcine cytB gene but does not cut beef cytB gene, all meatball contain porcine contamination give small DNA fragment (+ 220 and 130 bp) in electrophoresis analysis, while the absent of these DNA fragment leading to conclusion that meatball sample free from porcine. This method has been validated to detect up to 1% of porcine present in the meatball. 2010-10-14 Conference or Workshop Item NonPeerReviewed application/pdf en https://repository.ugm.ac.id/275133/1/ICCS%20Tri%20Joko%20R.pdf Raharjo, Tri Joko and Sismindari, Sismindari (2010) Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis). In: The 2nd International Conference On Chemical Sciences (2nd ICCS-2010), 14-16 October, Yogyakarta. |
| spellingShingle | Analytical Chemistry not elsewhere classified Raharjo, Tri Joko Sismindari, Sismindari Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) |
| title | Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) |
| title_full | Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) |
| title_fullStr | Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) |
| title_full_unstemmed | Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) |
| title_short | Validation of RFLP-Combined PCR Technique to Detect Porcine Contamination in Meatball (Halal Analysis) |
| title_sort | validation of rflp combined pcr technique to detect porcine contamination in meatball halal analysis |
| topic | Analytical Chemistry not elsewhere classified |
| url | https://repository.ugm.ac.id/275133/1/ICCS%20Tri%20Joko%20R.pdf |
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