Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats
Melanocortin-4 receptor (MC4R), a prototypical G-protein coupled receptor (GPCR) that is highly expressed in the hypothalamus plays an important role in regulating feed intake and energy balance. The aim of this study was to identify the association between single nucleotide polymorphism (SNP) in...
| Main Authors: | , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2019
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/276055/1/ISAG%20Latifah%202019.pdf |
| _version_ | 1797037047983112192 |
|---|---|
| author | Latifah, Latifah Kustantinah, Kustantinah Maharani, Dyah Hartatik, Tety |
| author_facet | Latifah, Latifah Kustantinah, Kustantinah Maharani, Dyah Hartatik, Tety |
| author_sort | Latifah, Latifah |
| collection | UGM |
| description | Melanocortin-4 receptor (MC4R), a prototypical G-protein coupled receptor (GPCR) that is highly expressed in the hypothalamus
plays an important role in regulating feed intake and energy balance.
The aim of this study was to identify the association between single nucleotide polymorphism (SNP) in MC4R gene with dry matter and crude
protein intake in post-weaned Bligon goats. The dry matter and crude
protein intake were estimated by in vivo method. Genomic DNA from
Seventeen Bligon goats was extracted for genotyping analysis using
polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP). One SNP (g.1079C/T) was identified by PCR-RFLP with
Kpn1 restriction enzyme. The results revealed 2 genotypes (CC and
CT), with frequencies of 0.71 and 0.29, respectively. Statistical analysis showed no significant association between MC4R genotype and dry
matter and crude protein intake. In conclusion, SNP g.1079C/T in the
MC4R gene is only useful for animal genotyping, but it cannot be used
as a marker-assisted selection on dry matter and crude protein intake in
Bligon goats. |
| first_indexed | 2024-03-13T23:56:26Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:276055 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:56:26Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2760552020-04-29T02:49:20Z https://repository.ugm.ac.id/276055/ Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats Latifah, Latifah Kustantinah, Kustantinah Maharani, Dyah Hartatik, Tety Gene Expression (incl. Microarray and other genome-wide approaches) Animal Breeding Melanocortin-4 receptor (MC4R), a prototypical G-protein coupled receptor (GPCR) that is highly expressed in the hypothalamus plays an important role in regulating feed intake and energy balance. The aim of this study was to identify the association between single nucleotide polymorphism (SNP) in MC4R gene with dry matter and crude protein intake in post-weaned Bligon goats. The dry matter and crude protein intake were estimated by in vivo method. Genomic DNA from Seventeen Bligon goats was extracted for genotyping analysis using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). One SNP (g.1079C/T) was identified by PCR-RFLP with Kpn1 restriction enzyme. The results revealed 2 genotypes (CC and CT), with frequencies of 0.71 and 0.29, respectively. Statistical analysis showed no significant association between MC4R genotype and dry matter and crude protein intake. In conclusion, SNP g.1079C/T in the MC4R gene is only useful for animal genotyping, but it cannot be used as a marker-assisted selection on dry matter and crude protein intake in Bligon goats. 2019-07-07 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/276055/1/ISAG%20Latifah%202019.pdf Latifah, Latifah and Kustantinah, Kustantinah and Maharani, Dyah and Hartatik, Tety (2019) Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats. In: the 37th International Conference on Animal Genetics 2019, 7-12 Juli 2019, Lleida, Spain. |
| spellingShingle | Gene Expression (incl. Microarray and other genome-wide approaches) Animal Breeding Latifah, Latifah Kustantinah, Kustantinah Maharani, Dyah Hartatik, Tety Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats |
| title | Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats |
| title_full | Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats |
| title_fullStr | Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats |
| title_full_unstemmed | Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats |
| title_short | Identification of polymorphism in MC4R gene and its association with dry matter and crude protein intake in post-weaned Bligon goats |
| title_sort | identification of polymorphism in mc4r gene and its association with dry matter and crude protein intake in post weaned bligon goats |
| topic | Gene Expression (incl. Microarray and other genome-wide approaches) Animal Breeding |
| url | https://repository.ugm.ac.id/276055/1/ISAG%20Latifah%202019.pdf |
| work_keys_str_mv | AT latifahlatifah identificationofpolymorphisminmc4rgeneanditsassociationwithdrymatterandcrudeproteinintakeinpostweanedbligongoats AT kustantinahkustantinah identificationofpolymorphisminmc4rgeneanditsassociationwithdrymatterandcrudeproteinintakeinpostweanedbligongoats AT maharanidyah identificationofpolymorphisminmc4rgeneanditsassociationwithdrymatterandcrudeproteinintakeinpostweanedbligongoats AT hartatiktety identificationofpolymorphisminmc4rgeneanditsassociationwithdrymatterandcrudeproteinintakeinpostweanedbligongoats |