Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck
Abstract. Prolactin plays important roles in avian reproduction as it induces broody behavior and regulates follicular development. The objectives of this study were to detect Single Nucleotide Polymorphisms (SNPs) of prolactin gene in Alabio and Mojosari ducks and determine the restriction enzym...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/276315/1/ISTAP%20ISTI_SNP_Lengkap.pdf |
| _version_ | 1797037065824632832 |
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| author | Damayanti, Isti Maharani, Dyah Sudaryati, Sri |
| author_facet | Damayanti, Isti Maharani, Dyah Sudaryati, Sri |
| author_sort | Damayanti, Isti |
| collection | UGM |
| description | Abstract. Prolactin plays important roles in avian reproduction as it induces broody behavior and
regulates follicular development. The objectives of this study were to detect Single Nucleotide
Polymorphisms (SNPs) of prolactin gene in Alabio and Mojosari ducks and determine the
restriction enzymes for genotyping. Genomic DNA was isolated from 50 blood samples per each
Alabio and Mojosari ducks. PCR amplification and sequencing were carried out to identify the
SNP. In this study, SNP T-6068C was found based on three GenBank sequences alignment. Two
SNPs (C-5796A and T-5817C) in intron 4 region were detected based on sequences of Alabio and
Mojosari PCR products. Enzymes Fok1, BtsCI, BsrI were detected to recognize the SNP C-
5796A. SNP T-6068C can be digested by enzym TspDTI. However no enzym was detected to
recognize SNP T-5817C. In conclusion the SNPs detected from this study may be used in future
studies to investigate the association of prolactin gene and egg production traits in Alabio and
Mojosari ducks. |
| first_indexed | 2024-03-13T23:57:13Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:276315 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T23:57:13Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2763152020-11-09T00:50:51Z https://repository.ugm.ac.id/276315/ Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck Damayanti, Isti Maharani, Dyah Sudaryati, Sri Animal Production Animal Breeding Abstract. Prolactin plays important roles in avian reproduction as it induces broody behavior and regulates follicular development. The objectives of this study were to detect Single Nucleotide Polymorphisms (SNPs) of prolactin gene in Alabio and Mojosari ducks and determine the restriction enzymes for genotyping. Genomic DNA was isolated from 50 blood samples per each Alabio and Mojosari ducks. PCR amplification and sequencing were carried out to identify the SNP. In this study, SNP T-6068C was found based on three GenBank sequences alignment. Two SNPs (C-5796A and T-5817C) in intron 4 region were detected based on sequences of Alabio and Mojosari PCR products. Enzymes Fok1, BtsCI, BsrI were detected to recognize the SNP C- 5796A. SNP T-6068C can be digested by enzym TspDTI. However no enzym was detected to recognize SNP T-5817C. In conclusion the SNPs detected from this study may be used in future studies to investigate the association of prolactin gene and egg production traits in Alabio and Mojosari ducks. 2019-09-23 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/276315/1/ISTAP%20ISTI_SNP_Lengkap.pdf Damayanti, Isti and Maharani, Dyah and Sudaryati, Sri (2019) Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck. In: ISTAP 2019, Yogyakarta, Indonesia. |
| spellingShingle | Animal Production Animal Breeding Damayanti, Isti Maharani, Dyah Sudaryati, Sri Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck |
| title | Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck |
| title_full | Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck |
| title_fullStr | Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck |
| title_full_unstemmed | Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck |
| title_short | Identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in Alabio and Mojosari duck |
| title_sort | identification of single nucleotide polimorphisms and restriction enzym on prolactin gene in alabio and mojosari duck |
| topic | Animal Production Animal Breeding |
| url | https://repository.ugm.ac.id/276315/1/ISTAP%20ISTI_SNP_Lengkap.pdf |
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