The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture
ABSTRACT Munira, Sunardi Radiono, Yohanes Widodo Wirohadidjojo - The effect of viamin C on fibroblast proliferation and vascular endothelial growth factor (VEGF) expression in normal fibroblast cell culture Background: One of the factors that determine the success of efficient woul')d healing i...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
2009
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| _version_ | 1797019761637326848 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
Munira, Sunardi Radiono, Yohanes Widodo Wirohadidjojo - The effect of viamin C on fibroblast proliferation and
vascular endothelial growth factor (VEGF) expression in normal fibroblast cell culture
Background: One of the factors that determine the success of efficient woul')d healing is wound healing rate, that
can be achieved by increasingcell proliferation, angiogenesisor neovascularisation,and extracellular matrix production.
Dermalfibroblast is a cell that plays an important role in wound healing. Fibroblast proliferation and neovascularisation
are critical elements in granular tissue formation. Local hypoxia causes fibroblasts to express HIF-1a that will induce
fibroblast VEGFexpression. The nature of vitamin C makes it easily oxidized. The addition of vitamin Con fibroblast
culture medium is expected to produce local hypoxia condition that will induce fibroblast expression of HIF-1a, so
that the expression of fibroblast VEGFwill be increased. Vitamin C may modulate the growth of various types of
cells. The effect of vitamin Con normal fibroblast proliferation and fibroblast VEGFexpression is still unknown.
Objective: This study was aimed to know whether vitamin Ccan increase normal human fibroblast proliferation and
expression of VEGF.
Method: A simple experimental study was conducted by using preputial skin fibroblast culture from 10-year-old
donor, subculture passage 3. Fibroblast culture was divided into 6 groups, each group received vitamin C treatment
with the dose of 50pg/mL, 100pg/mL, 150pg/mL, 200pg/mL, and 300pg/mL, and one group without treatment
acting as control. Measurement of fibroblast proliferation was conducted by spectrophotometer using MTT, and
fibroblast expression of VEGFwas measured using ELISA. The average of difference in fibroblast proliferation and
VEGFexpression was analyzed with one-way analysis of variance.
Result: There was a significant increase in fibroblast proliferation rate in the groups receiving vitamin C with the
dose of 200 mg/mL (p = 0.016) and 300 mg/mL (p = 0.005), whereas in the group with the dose of 50 mg/mL, 100
mg/mL and 150 mg/mL there was no significant difference compared to the control (p = 0.933, p = 0.961, P =
0.301, respectively). Average fibroblast VEGFexpression between various concentrations of vitamin C compared
to the control showed no significant difference (p > 0.05).
Conclusion: Vitamin Ccould be considered to be used as an agent to accelerate wounds healing.
Keywords: vitamin C, skin fibroblast culture, fibroblast proliferation, fibroblast VEGFexpression |
| first_indexed | 2024-03-05T23:11:25Z |
| format | Article |
| id | oai:generic.eprints.org:27740 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:04:41Z |
| publishDate | 2009 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:277402014-06-18T00:24:44Z https://repository.ugm.ac.id/27740/ The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Munira, Sunardi Radiono, Yohanes Widodo Wirohadidjojo - The effect of viamin C on fibroblast proliferation and vascular endothelial growth factor (VEGF) expression in normal fibroblast cell culture Background: One of the factors that determine the success of efficient woul')d healing is wound healing rate, that can be achieved by increasingcell proliferation, angiogenesisor neovascularisation,and extracellular matrix production. Dermalfibroblast is a cell that plays an important role in wound healing. Fibroblast proliferation and neovascularisation are critical elements in granular tissue formation. Local hypoxia causes fibroblasts to express HIF-1a that will induce fibroblast VEGFexpression. The nature of vitamin C makes it easily oxidized. The addition of vitamin Con fibroblast culture medium is expected to produce local hypoxia condition that will induce fibroblast expression of HIF-1a, so that the expression of fibroblast VEGFwill be increased. Vitamin C may modulate the growth of various types of cells. The effect of vitamin Con normal fibroblast proliferation and fibroblast VEGFexpression is still unknown. Objective: This study was aimed to know whether vitamin Ccan increase normal human fibroblast proliferation and expression of VEGF. Method: A simple experimental study was conducted by using preputial skin fibroblast culture from 10-year-old donor, subculture passage 3. Fibroblast culture was divided into 6 groups, each group received vitamin C treatment with the dose of 50pg/mL, 100pg/mL, 150pg/mL, 200pg/mL, and 300pg/mL, and one group without treatment acting as control. Measurement of fibroblast proliferation was conducted by spectrophotometer using MTT, and fibroblast expression of VEGFwas measured using ELISA. The average of difference in fibroblast proliferation and VEGFexpression was analyzed with one-way analysis of variance. Result: There was a significant increase in fibroblast proliferation rate in the groups receiving vitamin C with the dose of 200 mg/mL (p = 0.016) and 300 mg/mL (p = 0.005), whereas in the group with the dose of 50 mg/mL, 100 mg/mL and 150 mg/mL there was no significant difference compared to the control (p = 0.933, p = 0.961, P = 0.301, respectively). Average fibroblast VEGFexpression between various concentrations of vitamin C compared to the control showed no significant difference (p > 0.05). Conclusion: Vitamin Ccould be considered to be used as an agent to accelerate wounds healing. Keywords: vitamin C, skin fibroblast culture, fibroblast proliferation, fibroblast VEGFexpression [Yogyakarta] : Universitas Gadjah Mada 2009 Article NonPeerReviewed Perpustakaan UGM, i-lib (2009) The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=10803 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture |
| title | The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture |
| title_full | The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture |
| title_fullStr | The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture |
| title_full_unstemmed | The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture |
| title_short | The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture |
| title_sort | effect of vitamin c on fibroblast proliferation and vegfexpression in fibroblast culture |
| topic | Jurnal i-lib UGM |
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