Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep

Spermatozoa are easily damaged by lipid peroxidation during processing of semen. Therefore to minimize this damage and to protect spermatozoa from oxidative damage, antioxidants are used against the lipid peroxidation. Coconut water is characterized by its high contents of antioxidants. The benef...

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Main Authors: Bintara, Sigit, Andriyani, Astuti, Panjono, Panjono, Kusumastuti, Tri Anggraeni
Format: Conference or Workshop Item
Language:English
Published: 2018
Subjects:
Online Access:https://repository.ugm.ac.id/278085/1/Sigit%20Bintara%20et%20al_2018_Proceedings%20of%20the%2018th%20AAAP%20Animal%20Science%20Congress_227.pdf
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author Bintara, Sigit
Andriyani, Astuti
Panjono, Panjono
Kusumastuti, Tri Anggraeni
author_facet Bintara, Sigit
Andriyani, Astuti
Panjono, Panjono
Kusumastuti, Tri Anggraeni
author_sort Bintara, Sigit
collection UGM
description Spermatozoa are easily damaged by lipid peroxidation during processing of semen. Therefore to minimize this damage and to protect spermatozoa from oxidative damage, antioxidants are used against the lipid peroxidation. Coconut water is characterized by its high contents of antioxidants. The beneficial effects of Vitamin E as antioxidants on coconut water diluent of semen was researched. In this study the most active and available form of vitamin E, the α-tocopherol was used. Semen samples were collected from 12 heads of Fat-Tailed Sheep twice a week for 4 weeks using artificial vagina. The pooled semen samples were diluted with coconut water and Vitamin E (0.4 g/100 ml). The control consisted of coconut water diluent without vitamin E. The results showed that the motility was higher (P<0.05) in coconut water diluent with Vitamin E (50.8 ± 2.9%) compared to the control (46.8 ± 3.2%). Viability was higher (P<0.05) in the coconut water diluent with vitamin E (55.1 ± 2.8%) compared to the control (50.2 ± 3.3%). Intact plasma membrane was higher (P<0.05) in the coconut water diluent with vitamin E (44.8 ± 3.8%) compared to the control (42.8 ± 2.7%). This study concluded that additional Vitamin E as antioxidants on coconut water diluent can improve the quality of frozen semen i.e. highest values of motility, viability, and intact plasma membrane. This could make artificial insemination more successful. Successful artificial insemination is important to keep the valuable genetic of our local breeds of sheep, namely Fat-Tailed Sheep.
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spelling oai:generic.eprints.org:2780852023-03-16T01:32:27Z https://repository.ugm.ac.id/278085/ Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep Bintara, Sigit Andriyani, Astuti Panjono, Panjono Kusumastuti, Tri Anggraeni Animal Reproduction Spermatozoa are easily damaged by lipid peroxidation during processing of semen. Therefore to minimize this damage and to protect spermatozoa from oxidative damage, antioxidants are used against the lipid peroxidation. Coconut water is characterized by its high contents of antioxidants. The beneficial effects of Vitamin E as antioxidants on coconut water diluent of semen was researched. In this study the most active and available form of vitamin E, the α-tocopherol was used. Semen samples were collected from 12 heads of Fat-Tailed Sheep twice a week for 4 weeks using artificial vagina. The pooled semen samples were diluted with coconut water and Vitamin E (0.4 g/100 ml). The control consisted of coconut water diluent without vitamin E. The results showed that the motility was higher (P<0.05) in coconut water diluent with Vitamin E (50.8 ± 2.9%) compared to the control (46.8 ± 3.2%). Viability was higher (P<0.05) in the coconut water diluent with vitamin E (55.1 ± 2.8%) compared to the control (50.2 ± 3.3%). Intact plasma membrane was higher (P<0.05) in the coconut water diluent with vitamin E (44.8 ± 3.8%) compared to the control (42.8 ± 2.7%). This study concluded that additional Vitamin E as antioxidants on coconut water diluent can improve the quality of frozen semen i.e. highest values of motility, viability, and intact plasma membrane. This could make artificial insemination more successful. Successful artificial insemination is important to keep the valuable genetic of our local breeds of sheep, namely Fat-Tailed Sheep. 2018-08-05 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/278085/1/Sigit%20Bintara%20et%20al_2018_Proceedings%20of%20the%2018th%20AAAP%20Animal%20Science%20Congress_227.pdf Bintara, Sigit and Andriyani, Astuti and Panjono, Panjono and Kusumastuti, Tri Anggraeni (2018) Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep. In: 18th AAAP Congress 2018, 1-5 August 2018, Kuching, Malaysia.
spellingShingle Animal Reproduction
Bintara, Sigit
Andriyani, Astuti
Panjono, Panjono
Kusumastuti, Tri Anggraeni
Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep
title Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep
title_full Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep
title_fullStr Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep
title_full_unstemmed Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep
title_short Effect of Addition of Vitamin E on Coconut Water Diluent In Frozen Semen Quality of Fat-Tailed Sheep
title_sort effect of addition of vitamin e on coconut water diluent in frozen semen quality of fat tailed sheep
topic Animal Reproduction
url https://repository.ugm.ac.id/278085/1/Sigit%20Bintara%20et%20al_2018_Proceedings%20of%20the%2018th%20AAAP%20Animal%20Science%20Congress_227.pdf
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