Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat

Many mammalian species, including felids species, are threatened or endangered. As with all animals, species within felidae provide a larger benefit to the ecosystem and to humans while pursuing their own individual purpose of survival, maintenance, and reproduction. To prevent further extinction, i...

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Main Authors: Gustari, Sri, Budhi, Setyo, Karja, Ni Wayan Kurniani, Wlbowo, Hermawan Andrl, Purwo, Hardi, Yulianti, Ervina
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
Online Access:https://repository.ugm.ac.id/278243/1/FULL%20DOKUMEN%20-%20Prosiding%202014%20-%20Fertilizing%20Ability.pdf
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author Gustari, Sri
Budhi, Setyo
Karja, Ni Wayan Kurniani
Wlbowo, Hermawan Andrl
Purwo, Hardi
Yulianti, Ervina
author_facet Gustari, Sri
Budhi, Setyo
Karja, Ni Wayan Kurniani
Wlbowo, Hermawan Andrl
Purwo, Hardi
Yulianti, Ervina
author_sort Gustari, Sri
collection UGM
description Many mammalian species, including felids species, are threatened or endangered. As with all animals, species within felidae provide a larger benefit to the ecosystem and to humans while pursuing their own individual purpose of survival, maintenance, and reproduction. To prevent further extinction, it is appears to be justified to bring modern reproductive techniques to assist reproduction and conservation of the genetic resources of species threatened by extinction. Much progress has been made during the last decade towards developing assisted reproductive technologies for use in captive breeding programmes for conserving or enhancing the genetic diversity of threatened and endangered felids [1,2]. Since a limited number of endangered felid female and male gametes are available, the domestic cat has served as a convenient research model species for endangered felids [3]. It has been reported that spermatozoa collected from epididymides stored at 4 C'for a few days can maintain their motility (pig [4]; horse and shika deer [5]) and that they can be frozen and retain the ability to fertilize oocytes in vitro (pig [4]; monkey [6]). Post-mortem spermatozoa recovery is an important technique for obtaining germplasm reserves from genetically valuable animals or endangered species. However, there are many factors that influence the outcome of this technique. In this research, we studied whether spermatozoa within epididymides stored at 4 °C remain their motility and are able to penetrate the feline oocytes in vitro.
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spelling oai:generic.eprints.org:2782432023-05-10T00:52:11Z https://repository.ugm.ac.id/278243/ Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat Gustari, Sri Budhi, Setyo Karja, Ni Wayan Kurniani Wlbowo, Hermawan Andrl Purwo, Hardi Yulianti, Ervina Animal Developmental and Reproductive Biology Many mammalian species, including felids species, are threatened or endangered. As with all animals, species within felidae provide a larger benefit to the ecosystem and to humans while pursuing their own individual purpose of survival, maintenance, and reproduction. To prevent further extinction, it is appears to be justified to bring modern reproductive techniques to assist reproduction and conservation of the genetic resources of species threatened by extinction. Much progress has been made during the last decade towards developing assisted reproductive technologies for use in captive breeding programmes for conserving or enhancing the genetic diversity of threatened and endangered felids [1,2]. Since a limited number of endangered felid female and male gametes are available, the domestic cat has served as a convenient research model species for endangered felids [3]. It has been reported that spermatozoa collected from epididymides stored at 4 C'for a few days can maintain their motility (pig [4]; horse and shika deer [5]) and that they can be frozen and retain the ability to fertilize oocytes in vitro (pig [4]; monkey [6]). Post-mortem spermatozoa recovery is an important technique for obtaining germplasm reserves from genetically valuable animals or endangered species. However, there are many factors that influence the outcome of this technique. In this research, we studied whether spermatozoa within epididymides stored at 4 °C remain their motility and are able to penetrate the feline oocytes in vitro. 2014-10-13 Conference or Workshop Item PeerReviewed application/pdf en https://repository.ugm.ac.id/278243/1/FULL%20DOKUMEN%20-%20Prosiding%202014%20-%20Fertilizing%20Ability.pdf Gustari, Sri and Budhi, Setyo and Karja, Ni Wayan Kurniani and Wlbowo, Hermawan Andrl and Purwo, Hardi and Yulianti, Ervina (2014) Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat. In: The 14th Annual Workshop of The Regional Network of The Regional Network on Asian Schistosomasis and Other Helminth Zoonosis, 13-15 October 2014, IPB International Convention Center, Bogor, Indonesia.
spellingShingle Animal Developmental and Reproductive Biology
Gustari, Sri
Budhi, Setyo
Karja, Ni Wayan Kurniani
Wlbowo, Hermawan Andrl
Purwo, Hardi
Yulianti, Ervina
Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat
title Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat
title_full Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat
title_fullStr Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat
title_full_unstemmed Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat
title_short Fertilizing Ability of Post-Thaw of Epididymal Spermatozoa Stored for 48 H at 4derajat C Prior Cryopreservation in Domestic Cat
title_sort fertilizing ability of post thaw of epididymal spermatozoa stored for 48 h at 4derajat c prior cryopreservation in domestic cat
topic Animal Developmental and Reproductive Biology
url https://repository.ugm.ac.id/278243/1/FULL%20DOKUMEN%20-%20Prosiding%202014%20-%20Fertilizing%20Ability.pdf
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