Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia
Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the prevalence and the presence of the aerolysin gene and antimicrob...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022
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| Online Access: | https://repository.ugm.ac.id/278646/1/Purnomo_KH.pdf |
| _version_ | 1826050295001513984 |
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| author | Fikri, Faisal Wardhana, Dhandy Koesoemo Purnomo, Agus Khairani, Shafia Chhetri, Shekhar Purnama, Muhammad Thohawi Elziyad |
| author_facet | Fikri, Faisal Wardhana, Dhandy Koesoemo Purnomo, Agus Khairani, Shafia Chhetri, Shekhar Purnama, Muhammad Thohawi Elziyad |
| author_sort | Fikri, Faisal |
| collection | UGM |
| description | Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the
prevalence and the presence of the aerolysin gene and antimicrobial resistance profile of A. hydrophila isolated from milkfish in Gresik, Indonesia. Materials and Methods: A total of 153 milkfish gill samples were collected from 16 locations in Gresik and then cultured and identified using biochemical tests. The aerolysin gene was investigated using a polymerase chain reaction, and antimicrobial resistance profiles of the recovered isolates were investigated.
Results: Of the 153 examined samples, 35 (22.9%) were confirmed positive for A. hydrophila and 22 (62.9%) presented the aerolysin gene. The recovered isolates were resistant to the following antibiotics: Amoxicillin (62.9%), tetracycline (60%), streptomycin (54.3%), cefotaxime (51.4%), gentamycin (31.4%), kanamycin (28.6%), erythromycin (25.7%), chloramphenicol (20%), and trimethoprim (14.3%). Meanwhile, only ciprofloxacin, nalidixic acid, and imipenem were indicated as susceptible. Conclusion: The presence of the aerolysin gene is vital in determining the virulence of A. hydrophila. The study results indicated a high aerolysin gene prevalence. In addition, this study emphasized antibiotic use monitoring, food safety improvement, and negative impact reduction on human health and the environment. |
| first_indexed | 2024-03-14T00:01:43Z |
| format | Article |
| id | oai:generic.eprints.org:278646 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:01:43Z |
| publishDate | 2022 |
| publisher | Veterinary World |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2786462023-11-02T01:29:48Z https://repository.ugm.ac.id/278646/ Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia Fikri, Faisal Wardhana, Dhandy Koesoemo Purnomo, Agus Khairani, Shafia Chhetri, Shekhar Purnama, Muhammad Thohawi Elziyad Veterinary Sciences Background and Aim: Motile Aeromonas septicemia is a crucial disease in freshwater fish. Aeromonas hydrophila is a disease agent associated with sporadic fish mortality, food safety, and public health. This study aimed to estimate the prevalence and the presence of the aerolysin gene and antimicrobial resistance profile of A. hydrophila isolated from milkfish in Gresik, Indonesia. Materials and Methods: A total of 153 milkfish gill samples were collected from 16 locations in Gresik and then cultured and identified using biochemical tests. The aerolysin gene was investigated using a polymerase chain reaction, and antimicrobial resistance profiles of the recovered isolates were investigated. Results: Of the 153 examined samples, 35 (22.9%) were confirmed positive for A. hydrophila and 22 (62.9%) presented the aerolysin gene. The recovered isolates were resistant to the following antibiotics: Amoxicillin (62.9%), tetracycline (60%), streptomycin (54.3%), cefotaxime (51.4%), gentamycin (31.4%), kanamycin (28.6%), erythromycin (25.7%), chloramphenicol (20%), and trimethoprim (14.3%). Meanwhile, only ciprofloxacin, nalidixic acid, and imipenem were indicated as susceptible. Conclusion: The presence of the aerolysin gene is vital in determining the virulence of A. hydrophila. The study results indicated a high aerolysin gene prevalence. In addition, this study emphasized antibiotic use monitoring, food safety improvement, and negative impact reduction on human health and the environment. Veterinary World 2022-07-23 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/278646/1/Purnomo_KH.pdf Fikri, Faisal and Wardhana, Dhandy Koesoemo and Purnomo, Agus and Khairani, Shafia and Chhetri, Shekhar and Purnama, Muhammad Thohawi Elziyad (2022) Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia. Veterinary World, 15 (7). pp. 1759-1764. ISSN 2231-0916 http://veterinaryworld.org/Vol.15/July-2022/21.pdf http://doi.org/10.14202/vetworld.2022.1759-1764 |
| spellingShingle | Veterinary Sciences Fikri, Faisal Wardhana, Dhandy Koesoemo Purnomo, Agus Khairani, Shafia Chhetri, Shekhar Purnama, Muhammad Thohawi Elziyad Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia |
| title | Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia |
| title_full | Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia |
| title_fullStr | Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia |
| title_full_unstemmed | Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia |
| title_short | Aerolysin gene characterization and antimicrobial resistance profile of Aeromonas hydrophila isolated from milkfish (Chanos chanos) in Gresik, Indonesia |
| title_sort | aerolysin gene characterization and antimicrobial resistance profile of aeromonas hydrophila isolated from milkfish chanos chanos in gresik indonesia |
| topic | Veterinary Sciences |
| url | https://repository.ugm.ac.id/278646/1/Purnomo_KH.pdf |
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