Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound
Incorporating antimicrobial components into food packaging materials can prevent microbial contamination. Fungus combs could be an alternative source of natural antimicrobial agents. In this study, n-hexane, ethyl acetate, methanol, and water extracts were obtained from fungus combs isolated from I...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer
2022
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| Online Access: | https://repository.ugm.ac.id/278686/1/Witasari_TP.pdf |
| _version_ | 1797037330222022656 |
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| author | Witasari, Lucia Dhiantika Wahyu, Khairunnasa Wizdjanul Anugrahani, Bonifasia Junita Kurniawan, Dina Clarissa Haryanto, Aris Nandika, Dodi Karlinasari, Lina Arinana, Arinana Batubara, Irmanida Santoso, Djoko Rachmayanti, Yanti Firmansyah, Dikhi Sudiana, I Ketut Hertanto, Decsa Medika |
| author_facet | Witasari, Lucia Dhiantika Wahyu, Khairunnasa Wizdjanul Anugrahani, Bonifasia Junita Kurniawan, Dina Clarissa Haryanto, Aris Nandika, Dodi Karlinasari, Lina Arinana, Arinana Batubara, Irmanida Santoso, Djoko Rachmayanti, Yanti Firmansyah, Dikhi Sudiana, I Ketut Hertanto, Decsa Medika |
| author_sort | Witasari, Lucia Dhiantika |
| collection | UGM |
| description | Incorporating antimicrobial components into food packaging materials can prevent microbial contamination. Fungus
combs could be an alternative source of natural antimicrobial agents. In this study, n-hexane, ethyl acetate, methanol, and water extracts were obtained from fungus combs isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound. Their antibacterial and antifungal activities against food spoilage microorganisms including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Aspergillus flavus, and Aspergillus niger were evaluated by Kirby–Bauer disc diffusion and microdilution. Results showed that ethyl acetate extract formed the largest diameter inhibition zone for all tested bacteria and fungi, exhibited antibacterial activity against all tested bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.39 and 0.78 mg/mL, respectively, and suppressed A. flavus and A. niger with an MIC value of 0.78 mg/mL. This extract contained guaiacol and syringol, which were predicted as the main antimicrobial components in fungus comb. n-Hexane extract only inhibited Gram-positive bacteria. S. aureus ATCC 25923 was the most sensitive to all the extracts, and A. flavus was more sensitive than A. niger. All these fungus comb extracts exhibited antimicrobial activity against E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC 25923, A. flavus, and A. niger. This study revealed that fungus comb extracts, especially ethyl acetate, could be considered as a new antimicrobial agent. |
| first_indexed | 2024-03-14T00:01:50Z |
| format | Article |
| id | oai:generic.eprints.org:278686 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:01:50Z |
| publishDate | 2022 |
| publisher | Springer |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2786862023-11-02T01:00:53Z https://repository.ugm.ac.id/278686/ Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound Witasari, Lucia Dhiantika Wahyu, Khairunnasa Wizdjanul Anugrahani, Bonifasia Junita Kurniawan, Dina Clarissa Haryanto, Aris Nandika, Dodi Karlinasari, Lina Arinana, Arinana Batubara, Irmanida Santoso, Djoko Rachmayanti, Yanti Firmansyah, Dikhi Sudiana, I Ketut Hertanto, Decsa Medika Food Engineering Incorporating antimicrobial components into food packaging materials can prevent microbial contamination. Fungus combs could be an alternative source of natural antimicrobial agents. In this study, n-hexane, ethyl acetate, methanol, and water extracts were obtained from fungus combs isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound. Their antibacterial and antifungal activities against food spoilage microorganisms including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Aspergillus flavus, and Aspergillus niger were evaluated by Kirby–Bauer disc diffusion and microdilution. Results showed that ethyl acetate extract formed the largest diameter inhibition zone for all tested bacteria and fungi, exhibited antibacterial activity against all tested bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.39 and 0.78 mg/mL, respectively, and suppressed A. flavus and A. niger with an MIC value of 0.78 mg/mL. This extract contained guaiacol and syringol, which were predicted as the main antimicrobial components in fungus comb. n-Hexane extract only inhibited Gram-positive bacteria. S. aureus ATCC 25923 was the most sensitive to all the extracts, and A. flavus was more sensitive than A. niger. All these fungus comb extracts exhibited antimicrobial activity against E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC 25923, A. flavus, and A. niger. This study revealed that fungus comb extracts, especially ethyl acetate, could be considered as a new antimicrobial agent. Springer 2022-02-10 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/278686/1/Witasari_TP.pdf Witasari, Lucia Dhiantika and Wahyu, Khairunnasa Wizdjanul and Anugrahani, Bonifasia Junita and Kurniawan, Dina Clarissa and Haryanto, Aris and Nandika, Dodi and Karlinasari, Lina and Arinana, Arinana and Batubara, Irmanida and Santoso, Djoko and Rachmayanti, Yanti and Firmansyah, Dikhi and Sudiana, I Ketut and Hertanto, Decsa Medika (2022) Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound. AMB Express, 2022 (12). pp. 1-10. ISSN 2191-0855 https://amb-express.springeropen.com/articles/10.1186/s13568-022-01359-0 https://doi.org/10.1186/s13568-022-01359-0 |
| spellingShingle | Food Engineering Witasari, Lucia Dhiantika Wahyu, Khairunnasa Wizdjanul Anugrahani, Bonifasia Junita Kurniawan, Dina Clarissa Haryanto, Aris Nandika, Dodi Karlinasari, Lina Arinana, Arinana Batubara, Irmanida Santoso, Djoko Rachmayanti, Yanti Firmansyah, Dikhi Sudiana, I Ketut Hertanto, Decsa Medika Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound |
| title | Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound |
| title_full | Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound |
| title_fullStr | Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound |
| title_full_unstemmed | Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound |
| title_short | Antimicrobial activities of fungus comb extracts isolated from Indomalayan termite (Macrotermes gilvus Hagen) mound |
| title_sort | antimicrobial activities of fungus comb extracts isolated from indomalayan termite macrotermes gilvus hagen mound |
| topic | Food Engineering |
| url | https://repository.ugm.ac.id/278686/1/Witasari_TP.pdf |
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