Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia
Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology (JPAM)
2022
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| Online Access: | https://repository.ugm.ac.id/278767/1/Krisnawati_TP.pdf |
| _version_ | 1826050317710524416 |
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| author | Krisnawati, Ririn Sardjono, Sardjono Widada, Jaka Suroto, Dian Anggraini Cahyanto, Muhammad Nur |
| author_facet | Krisnawati, Ririn Sardjono, Sardjono Widada, Jaka Suroto, Dian Anggraini Cahyanto, Muhammad Nur |
| author_sort | Krisnawati, Ririn |
| collection | UGM |
| description | Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endoxylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT 2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021-0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined. © The Author(s) 2022. |
| first_indexed | 2024-03-14T00:02:04Z |
| format | Article |
| id | oai:generic.eprints.org:278767 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:02:04Z |
| publishDate | 2022 |
| publisher | Journal of Pure and Applied Microbiology (JPAM) |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2787672023-10-11T08:11:27Z https://repository.ugm.ac.id/278767/ Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia Krisnawati, Ririn Sardjono, Sardjono Widada, Jaka Suroto, Dian Anggraini Cahyanto, Muhammad Nur Microbiology Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endoxylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT 2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021-0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined. © The Author(s) 2022. Journal of Pure and Applied Microbiology (JPAM) 2022-01-10 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/278767/1/Krisnawati_TP.pdf Krisnawati, Ririn and Sardjono, Sardjono and Widada, Jaka and Suroto, Dian Anggraini and Cahyanto, Muhammad Nur (2022) Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia. Journal of Pure and Applied Microbiology (JPAM), 16 (1). pp. 226-234. ISSN 2581-690X https://microbiologyjournal.org/effect-of-glucose-on-endo-xylanase-and-%ce%b2-xylosidase-production-by-fungi-isolated-in-indonesia/ https://doi.org/10.22207/JPAM.16.1.12 |
| spellingShingle | Microbiology Krisnawati, Ririn Sardjono, Sardjono Widada, Jaka Suroto, Dian Anggraini Cahyanto, Muhammad Nur Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_full | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_fullStr | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_full_unstemmed | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_short | Effect of Glucose on Endo-xylanase and β-xylosidase Production by Fungi Isolated in Indonesia |
| title_sort | effect of glucose on endo xylanase and β xylosidase production by fungi isolated in indonesia |
| topic | Microbiology |
| url | https://repository.ugm.ac.id/278767/1/Krisnawati_TP.pdf |
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