Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications
Background: Chronic hyperglycaemia of diabetes causes long-term damage and impaired function of multiple organs. However, the pathological changes in the liver following long-term diabetes remain unclear. This study aimed to determine the pathological complications of longterm diabetes in the rat li...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2022
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| Online Access: | https://repository.ugm.ac.id/279031/1/Yuniartha_KKMK.pdf |
| _version_ | 1797037407695011840 |
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| author | Yuniartha, Ratih Arfian, Nur Setyaningsih, Wiwit Ananda Wahyu Kencana, Sagita Mega Sekar Sari, Dwi Cahyani Ratna |
| author_facet | Yuniartha, Ratih Arfian, Nur Setyaningsih, Wiwit Ananda Wahyu Kencana, Sagita Mega Sekar Sari, Dwi Cahyani Ratna |
| author_sort | Yuniartha, Ratih |
| collection | UGM |
| description | Background: Chronic hyperglycaemia of diabetes causes long-term damage and impaired function of multiple organs. However, the pathological changes in the liver following long-term diabetes remain unclear. This study aimed to determine the pathological complications of longterm diabetes in the rat liver. Methods: Intraperitoneal injection of streptozotocin (STZ) was used to induce diabetes in rats at a single dose (60 mg/kg body weight [BW]). Rats were euthanised at 1 month (DM1 group), 2 months (DM2 group) and 4 months (DM4 group) following diabetes induction with six rats in each group. Immunohistochemistry was performed against SOD1, CD68, p53 and p16 antibodies.
Messenger RNA (mRNA) expressions of SOD1, SOD2, GPx, CD68, p53, p21 and caspase-3 genes were measured by reverse transcription-polymerase chain reaction. Results: Hepatic p53 mRNA expression was significantly higher in DM1, DM2 and DM4 groups compared to the control group. The p21 and caspase-3 mRNA expressions were significantly upregulated in the DM2 and DM4 groups. The p16-positive cells were obviously increased, particularly in the DM4 group. Bivariate correlation analysis showed mRNA expressions of p21 and caspase-3 genes were positively correlated with the p53 gene. Conclusion: Diabetic rats exhibited increased apoptosis and senescence in the liver following a longer period of hyperglycaemia. |
| first_indexed | 2024-03-14T00:02:50Z |
| format | Article |
| id | oai:generic.eprints.org:279031 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:02:50Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Sains Malaysia |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2790312023-11-01T03:43:36Z https://repository.ugm.ac.id/279031/ Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications Yuniartha, Ratih Arfian, Nur Setyaningsih, Wiwit Ananda Wahyu Kencana, Sagita Mega Sekar Sari, Dwi Cahyani Ratna Clinical Sciences Background: Chronic hyperglycaemia of diabetes causes long-term damage and impaired function of multiple organs. However, the pathological changes in the liver following long-term diabetes remain unclear. This study aimed to determine the pathological complications of longterm diabetes in the rat liver. Methods: Intraperitoneal injection of streptozotocin (STZ) was used to induce diabetes in rats at a single dose (60 mg/kg body weight [BW]). Rats were euthanised at 1 month (DM1 group), 2 months (DM2 group) and 4 months (DM4 group) following diabetes induction with six rats in each group. Immunohistochemistry was performed against SOD1, CD68, p53 and p16 antibodies. Messenger RNA (mRNA) expressions of SOD1, SOD2, GPx, CD68, p53, p21 and caspase-3 genes were measured by reverse transcription-polymerase chain reaction. Results: Hepatic p53 mRNA expression was significantly higher in DM1, DM2 and DM4 groups compared to the control group. The p21 and caspase-3 mRNA expressions were significantly upregulated in the DM2 and DM4 groups. The p16-positive cells were obviously increased, particularly in the DM4 group. Bivariate correlation analysis showed mRNA expressions of p21 and caspase-3 genes were positively correlated with the p53 gene. Conclusion: Diabetic rats exhibited increased apoptosis and senescence in the liver following a longer period of hyperglycaemia. Penerbit Universiti Sains Malaysia 2022-12-22 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/279031/1/Yuniartha_KKMK.pdf Yuniartha, Ratih and Arfian, Nur and Setyaningsih, Wiwit Ananda Wahyu and Kencana, Sagita Mega Sekar and Sari, Dwi Cahyani Ratna (2022) Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications. Malays J Med Sci., 29 (6). pp. 46-59. ISSN 2180-4303 (In Press) http://www.mjms.usm.my/index.html https://doi.org/10.21315/mjms2022.29.6.5 |
| spellingShingle | Clinical Sciences Yuniartha, Ratih Arfian, Nur Setyaningsih, Wiwit Ananda Wahyu Kencana, Sagita Mega Sekar Sari, Dwi Cahyani Ratna Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications |
| title | Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications |
| title_full | Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications |
| title_fullStr | Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications |
| title_full_unstemmed | Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications |
| title_short | Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications |
| title_sort | accelerated senescence and apoptosis in the rat liver during the progression of diabetic complications |
| topic | Clinical Sciences |
| url | https://repository.ugm.ac.id/279031/1/Yuniartha_KKMK.pdf |
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