Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta
Background: Transmission of pathogenic and non-pathogenic microorganisms from animals to humans can affect the composition of gut bacteria (gut microbiome) in humans and can also be a potential source of transmission of zoonotic diseases. Therefore, this study aimed to analyze the gut microbiome int...
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| Format: | Article |
| Language: | English |
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Sanglah General Hospital
2022
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| Online Access: | https://repository.ugm.ac.id/279033/1/Gut%20microbiome%20analysis%20in%20human%20living%20close%20to%20livestock%20at%20Mlati%20district%2C%20Sleman%2C%20Yogyakarta.pdf |
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| author | Sari, Yulia Suryawati, Betty Probandari, Ari Hartono, Hartono Artama, Wayan Tunas Purwanto, Bambang Soetrisno, Soetrisno |
| author_facet | Sari, Yulia Suryawati, Betty Probandari, Ari Hartono, Hartono Artama, Wayan Tunas Purwanto, Bambang Soetrisno, Soetrisno |
| author_sort | Sari, Yulia |
| collection | UGM |
| description | Background: Transmission of pathogenic and non-pathogenic microorganisms from animals to humans can affect the composition of gut bacteria (gut microbiome) in humans and can also be a potential source of transmission of zoonotic diseases. Therefore, this study aimed to analyze the gut microbiome interactions between the human and the livestock. Method: This study was a cross-sectional study conducted in Mlati District, Slemanm Yogyakarta. The fecal sample was used to identify parasites by using trichrome staining. Then NGS selected samples were used to analyze the gut microbiome using Illumina high throughput 16S rRNA region V3-V4 sequencing. Result: The analysis showed that Bacteroidetes was prevalent in the HCA group (57%), while Firmicutes (70%) were commonly found in HNC. Regarding the potential parasitic infection, direct microscopic examination and trichrome staining showed that Blastocystis sp and Entamoeba coli cyst was found in several subjects in HCA groups, with prevalence rate at 7.69% and 2.5%. Meanwhile, no parasite was found in HNC feces. Conclusion: There was a significant difference in the gut microbiome and parasitic infections between the HCA and HNC groups, with Blastocystis sp and Entamoeba coli as the most prevalent parasites. |
| first_indexed | 2024-03-14T00:02:51Z |
| format | Article |
| id | oai:generic.eprints.org:279033 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:02:51Z |
| publishDate | 2022 |
| publisher | Sanglah General Hospital |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2790332023-10-30T04:15:04Z https://repository.ugm.ac.id/279033/ Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta Sari, Yulia Suryawati, Betty Probandari, Ari Hartono, Hartono Artama, Wayan Tunas Purwanto, Bambang Soetrisno, Soetrisno Livestock Public Health and Health Services Veterinary Sciences Background: Transmission of pathogenic and non-pathogenic microorganisms from animals to humans can affect the composition of gut bacteria (gut microbiome) in humans and can also be a potential source of transmission of zoonotic diseases. Therefore, this study aimed to analyze the gut microbiome interactions between the human and the livestock. Method: This study was a cross-sectional study conducted in Mlati District, Slemanm Yogyakarta. The fecal sample was used to identify parasites by using trichrome staining. Then NGS selected samples were used to analyze the gut microbiome using Illumina high throughput 16S rRNA region V3-V4 sequencing. Result: The analysis showed that Bacteroidetes was prevalent in the HCA group (57%), while Firmicutes (70%) were commonly found in HNC. Regarding the potential parasitic infection, direct microscopic examination and trichrome staining showed that Blastocystis sp and Entamoeba coli cyst was found in several subjects in HCA groups, with prevalence rate at 7.69% and 2.5%. Meanwhile, no parasite was found in HNC feces. Conclusion: There was a significant difference in the gut microbiome and parasitic infections between the HCA and HNC groups, with Blastocystis sp and Entamoeba coli as the most prevalent parasites. Sanglah General Hospital 2022-12-10 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/279033/1/Gut%20microbiome%20analysis%20in%20human%20living%20close%20to%20livestock%20at%20Mlati%20district%2C%20Sleman%2C%20Yogyakarta.pdf Sari, Yulia and Suryawati, Betty and Probandari, Ari and Hartono, Hartono and Artama, Wayan Tunas and Purwanto, Bambang and Soetrisno, Soetrisno (2022) Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta. Bali Medical Journal (Bali MedJ) 2022, 11 (3). pp. 1390-1396. ISSN 2302-2914 https://balimedicaljournal.org/index.php/bmj/article/view/3774 https://doi.org/10.15562/bmj.v11i3.3774 |
| spellingShingle | Livestock Public Health and Health Services Veterinary Sciences Sari, Yulia Suryawati, Betty Probandari, Ari Hartono, Hartono Artama, Wayan Tunas Purwanto, Bambang Soetrisno, Soetrisno Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta |
| title | Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta |
| title_full | Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta |
| title_fullStr | Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta |
| title_full_unstemmed | Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta |
| title_short | Gut microbiome analysis in human living close to livestock at Mlati district, Sleman, Yogyakarta |
| title_sort | gut microbiome analysis in human living close to livestock at mlati district sleman yogyakarta |
| topic | Livestock Public Health and Health Services Veterinary Sciences |
| url | https://repository.ugm.ac.id/279033/1/Gut%20microbiome%20analysis%20in%20human%20living%20close%20to%20livestock%20at%20Mlati%20district%2C%20Sleman%2C%20Yogyakarta.pdf |
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