Acute toxicity evaluation and immunomodulatory potential of hydrodynamic cavitation extract of citrus peels
Citrus peels have been explored vastly due to their high methoxy flavonoid contents that show several biological activities, including immunomodulation, anti-inflammation, and chemoprevention. A water-based extraction method, hydrodynamic cavitation (HC), has recently been adopted to obtain a high...
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| Format: | Article |
| Language: | English |
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Open Science Publishers LLP
2022
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| Online Access: | https://repository.ugm.ac.id/279091/1/Putri_FA.pdf |
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| author | Putri, Dyaningtyas Dewi Pamungkas Maran, Gergorius Gena Kusumastuti, Yuni Susidarti, Ratna Asmah Meiyanto, Edy Ikawati, Muthi' |
| author_facet | Putri, Dyaningtyas Dewi Pamungkas Maran, Gergorius Gena Kusumastuti, Yuni Susidarti, Ratna Asmah Meiyanto, Edy Ikawati, Muthi' |
| author_sort | Putri, Dyaningtyas Dewi Pamungkas |
| collection | UGM |
| description | Citrus peels have been explored vastly due to their high methoxy flavonoid contents that show several biological
activities, including immunomodulation, anti-inflammation, and chemoprevention. A water-based extraction method,
hydrodynamic cavitation (HC), has recently been adopted to obtain a high methoxy flavonoid extract from Citrus
reticulata peels in order to increase its acceptance and utilization. Aiming to verify the safety and immunomodulatory potency of this HC extract of citrus peels (HCE-CP), an acute toxicity test and immunomodulatory assay in rats were carried out. The single oral administration of HCE-CP did not show signs of either toxicity or death up to the 15th day even at the highest dosage, resulting in an lethal dose 50 of more than 5,000 mg/kg body weight (BW). In
immunomodulatory evaluation, HCE-CP was given orally daily for 11 weeks, induced with lipopolysaccharide (LPS), and the blood was sampled 5 hours after LPS induction. HCE-CP treatments increased the relative weight of the spleen. The dosage of 2,000 mg/kg BW reduced CD4+ T lymphocytes significantly, but not CD8+. Bioinformatics analysis predicted that hesperidin and hesperetin, two major methoxy flavonoids in HCE-CP, target several proteins that are involved in inflammation in LPS-induced lymphocytes. Taking these data together, HCE-CP is not toxic and shows immunomodulatory potency, especially anti-inflammation. |
| first_indexed | 2024-03-14T00:03:01Z |
| format | Article |
| id | oai:generic.eprints.org:279091 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:03:01Z |
| publishDate | 2022 |
| publisher | Open Science Publishers LLP |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2790912023-11-01T08:03:10Z https://repository.ugm.ac.id/279091/ Acute toxicity evaluation and immunomodulatory potential of hydrodynamic cavitation extract of citrus peels Putri, Dyaningtyas Dewi Pamungkas Maran, Gergorius Gena Kusumastuti, Yuni Susidarti, Ratna Asmah Meiyanto, Edy Ikawati, Muthi' Pharmaceutical Sciences Citrus peels have been explored vastly due to their high methoxy flavonoid contents that show several biological activities, including immunomodulation, anti-inflammation, and chemoprevention. A water-based extraction method, hydrodynamic cavitation (HC), has recently been adopted to obtain a high methoxy flavonoid extract from Citrus reticulata peels in order to increase its acceptance and utilization. Aiming to verify the safety and immunomodulatory potency of this HC extract of citrus peels (HCE-CP), an acute toxicity test and immunomodulatory assay in rats were carried out. The single oral administration of HCE-CP did not show signs of either toxicity or death up to the 15th day even at the highest dosage, resulting in an lethal dose 50 of more than 5,000 mg/kg body weight (BW). In immunomodulatory evaluation, HCE-CP was given orally daily for 11 weeks, induced with lipopolysaccharide (LPS), and the blood was sampled 5 hours after LPS induction. HCE-CP treatments increased the relative weight of the spleen. The dosage of 2,000 mg/kg BW reduced CD4+ T lymphocytes significantly, but not CD8+. Bioinformatics analysis predicted that hesperidin and hesperetin, two major methoxy flavonoids in HCE-CP, target several proteins that are involved in inflammation in LPS-induced lymphocytes. Taking these data together, HCE-CP is not toxic and shows immunomodulatory potency, especially anti-inflammation. Open Science Publishers LLP 2022-04 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/279091/1/Putri_FA.pdf Putri, Dyaningtyas Dewi Pamungkas and Maran, Gergorius Gena and Kusumastuti, Yuni and Susidarti, Ratna Asmah and Meiyanto, Edy and Ikawati, Muthi' (2022) Acute toxicity evaluation and immunomodulatory potential of hydrodynamic cavitation extract of citrus peels. Journal of Applied Pharmaceutical Science, 12 (4). pp. 136-145. ISSN 2231-3354 http://www.japsonline.com http://doi.org/10.7324/JAPS.2022.120415 |
| spellingShingle | Pharmaceutical Sciences Putri, Dyaningtyas Dewi Pamungkas Maran, Gergorius Gena Kusumastuti, Yuni Susidarti, Ratna Asmah Meiyanto, Edy Ikawati, Muthi' Acute toxicity evaluation and immunomodulatory potential of hydrodynamic cavitation extract of citrus peels |
| title | Acute toxicity evaluation and immunomodulatory potential of
hydrodynamic cavitation extract of citrus peels |
| title_full | Acute toxicity evaluation and immunomodulatory potential of
hydrodynamic cavitation extract of citrus peels |
| title_fullStr | Acute toxicity evaluation and immunomodulatory potential of
hydrodynamic cavitation extract of citrus peels |
| title_full_unstemmed | Acute toxicity evaluation and immunomodulatory potential of
hydrodynamic cavitation extract of citrus peels |
| title_short | Acute toxicity evaluation and immunomodulatory potential of
hydrodynamic cavitation extract of citrus peels |
| title_sort | acute toxicity evaluation and immunomodulatory potential of hydrodynamic cavitation extract of citrus peels |
| topic | Pharmaceutical Sciences |
| url | https://repository.ugm.ac.id/279091/1/Putri_FA.pdf |
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