| Summary: | ABSTRACT
Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was extracted and purified from acetone dry powder of
cocoa (Theobroma cacao. L) of clone PBC 159 extract. The Lipase from AcDP of cocoa beans was used for
purification using 40-60 and 60-80% ammonium sulphate precipitation. The resulted indicated 44.73 and 60.51-fold
purification with 26.74 and 33.31% recovery lipase activity (yield), respectively. The crude lipase enzyme from both
precipitation were eluted, producing a single peak after applying through Sephacryl S-200 chromatography. The
purified enzyme had a uniform specific activity throughout the final chromatography peak. The results from SDSPAGE
analysis showed that the molecular weight of the lipase enzyme was in between 45-66 kDa.
Keywords: isolation, purification, lipase, cocoa beans
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