ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159
ABSTRACT Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was extracted and purified from acetone dry powder of cocoa (Theobroma cacao. L) of clone PBC 159 extract. The Lipase from AcDP of cocoa beans was used for purification using 40-60 and 60-80% ammonium sulphate precipitation. The resulted in...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
2008
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| _version_ | 1826033123839705088 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was extracted and purified from acetone dry powder of
cocoa (Theobroma cacao. L) of clone PBC 159 extract. The Lipase from AcDP of cocoa beans was used for
purification using 40-60 and 60-80% ammonium sulphate precipitation. The resulted indicated 44.73 and 60.51-fold
purification with 26.74 and 33.31% recovery lipase activity (yield), respectively. The crude lipase enzyme from both
precipitation were eluted, producing a single peak after applying through Sephacryl S-200 chromatography. The
purified enzyme had a uniform specific activity throughout the final chromatography peak. The results from SDSPAGE
analysis showed that the molecular weight of the lipase enzyme was in between 45-66 kDa.
Keywords: isolation, purification, lipase, cocoa beans |
| first_indexed | 2024-03-13T19:05:12Z |
| format | Article |
| id | oai:generic.eprints.org:27911 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:05:12Z |
| publishDate | 2008 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:279112014-06-18T00:24:55Z https://repository.ugm.ac.id/27911/ ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was extracted and purified from acetone dry powder of cocoa (Theobroma cacao. L) of clone PBC 159 extract. The Lipase from AcDP of cocoa beans was used for purification using 40-60 and 60-80% ammonium sulphate precipitation. The resulted indicated 44.73 and 60.51-fold purification with 26.74 and 33.31% recovery lipase activity (yield), respectively. The crude lipase enzyme from both precipitation were eluted, producing a single peak after applying through Sephacryl S-200 chromatography. The purified enzyme had a uniform specific activity throughout the final chromatography peak. The results from SDSPAGE analysis showed that the molecular weight of the lipase enzyme was in between 45-66 kDa. Keywords: isolation, purification, lipase, cocoa beans [Yogyakarta] : Universitas Gadjah Mada 2008 Article NonPeerReviewed Perpustakaan UGM, i-lib (2008) ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=10974 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 |
| title | ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 |
| title_full | ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 |
| title_fullStr | ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 |
| title_full_unstemmed | ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 |
| title_short | ISOLATIONANDPURIFICATIONOF LIPASE FROMCOCOA BEANS (Theobroma cacao. L.)OF CLONE PBC 159 |
| title_sort | isolationandpurificationof lipase fromcocoa beans theobroma cacao l of clone pbc 159 |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib isolationandpurificationoflipasefromcocoabeanstheobromacacaolofclonepbc159 |