Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2
Amylomyces rouxii is commonly found as amylolytic fungi in tapai fermentation. However, its diversity is rarely reported despite being often used for food production in Southeast Asia. This research aims to analyze the genetic diversity and the distribution pattern of A. rouxii from Ragi tapai in Ja...
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| Format: | Article |
| Language: | English |
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Taylor and Francis Ltd.
2022
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| Online Access: | https://repository.ugm.ac.id/280259/1/Genetic%20Diversity%20of%20Amylomyces%20rouxii%20from%20Ragi%20tapai%20in%20Java%20Island%20Based%20on%20Ribosomal%20Regions%20ITS1%20ITS2%20and%20D1%20D2.pdf |
| _version_ | 1797037455293022208 |
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| author | Delva, Ega Arisuryanti, Tuty Ilmi, Miftahul |
| author_facet | Delva, Ega Arisuryanti, Tuty Ilmi, Miftahul |
| author_sort | Delva, Ega |
| collection | UGM |
| description | Amylomyces rouxii is commonly found as amylolytic fungi in tapai fermentation. However, its diversity is rarely reported despite being often used for food production in Southeast Asia. This research aims to analyze the genetic diversity and the distribution pattern of A. rouxii from Ragi tapai in Java Island, Indonesia. We isolated the fungus from samples obtained from Ragi tapai producing centers in Bandung, Sumedang, Muntilan, Blora, Yogyakarta, and Bondowoso. The obtained isolates were molecularly identified based on the ribosomal regions ITS1/ITS2 and D1/D2, then analyzed for phylogenetic tree reconstruction, genetic distance, genetic variation, and haplotype networking. Six isolates showed specific morphological traits of A. rouxii. However, phylogenetic tree reconstruction on the ribosomal genes showed that the isolates were grouped into two different clades related to two species. Clade A included BDG, SMD, and MTL isolates related to A. rouxii, whereas clade B included YOG, BLR, and BDS isolates related to Mucor indicus. The genetic distances between clades for ITS1/ITS2 and D1/D2 were 0.6145 and 0.1556, respectively. In conclusion, we confirmed the genetic diversity of molds from Ragi tapai in Java Island and showed that the isolates are not only related to A. rouxii as reported before. |
| first_indexed | 2024-03-14T00:03:35Z |
| format | Article |
| id | oai:generic.eprints.org:280259 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:03:35Z |
| publishDate | 2022 |
| publisher | Taylor and Francis Ltd. |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2802592023-11-07T07:36:12Z https://repository.ugm.ac.id/280259/ Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 Delva, Ega Arisuryanti, Tuty Ilmi, Miftahul Biological Sciences Amylomyces rouxii is commonly found as amylolytic fungi in tapai fermentation. However, its diversity is rarely reported despite being often used for food production in Southeast Asia. This research aims to analyze the genetic diversity and the distribution pattern of A. rouxii from Ragi tapai in Java Island, Indonesia. We isolated the fungus from samples obtained from Ragi tapai producing centers in Bandung, Sumedang, Muntilan, Blora, Yogyakarta, and Bondowoso. The obtained isolates were molecularly identified based on the ribosomal regions ITS1/ITS2 and D1/D2, then analyzed for phylogenetic tree reconstruction, genetic distance, genetic variation, and haplotype networking. Six isolates showed specific morphological traits of A. rouxii. However, phylogenetic tree reconstruction on the ribosomal genes showed that the isolates were grouped into two different clades related to two species. Clade A included BDG, SMD, and MTL isolates related to A. rouxii, whereas clade B included YOG, BLR, and BDS isolates related to Mucor indicus. The genetic distances between clades for ITS1/ITS2 and D1/D2 were 0.6145 and 0.1556, respectively. In conclusion, we confirmed the genetic diversity of molds from Ragi tapai in Java Island and showed that the isolates are not only related to A. rouxii as reported before. Taylor and Francis Ltd. 2022-02-27 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/280259/1/Genetic%20Diversity%20of%20Amylomyces%20rouxii%20from%20Ragi%20tapai%20in%20Java%20Island%20Based%20on%20Ribosomal%20Regions%20ITS1%20ITS2%20and%20D1%20D2.pdf Delva, Ega and Arisuryanti, Tuty and Ilmi, Miftahul (2022) Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2. MYCOBIOLOGY, 50 (2). pp. 132-141. ISSN 12298093 https://www.tandfonline.com/doi/full/10.1080/12298093.2022.2028436 https://doi.org/10.1080/12298093.2022.2028436 |
| spellingShingle | Biological Sciences Delva, Ega Arisuryanti, Tuty Ilmi, Miftahul Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 |
| title | Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 |
| title_full | Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 |
| title_fullStr | Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 |
| title_full_unstemmed | Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 |
| title_short | Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2 |
| title_sort | genetic diversity of amylomyces rouxii from ragi tapai in java island based on ribosomal regions its1 its2 and d1 d2 |
| topic | Biological Sciences |
| url | https://repository.ugm.ac.id/280259/1/Genetic%20Diversity%20of%20Amylomyces%20rouxii%20from%20Ragi%20tapai%20in%20Java%20Island%20Based%20on%20Ribosomal%20Regions%20ITS1%20ITS2%20and%20D1%20D2.pdf |
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