| Summary: | ABSTRACT
In recent years, instead of their potential hazard to human health, carbamic pesticides are widely used in
agriculture. Therefore, there is a growing interest in rapid and accurate determination for food safety and
environmental monitoring. The aim of this research is to designs a biosensor for analyzing carbamate pesticides
residue in sample with composite variables of glutaraldehyde (GA) concentration in electrode membrane. Enzyme acetylcholinesterase (AChE) was co-immobilised with choline oxidase (ChO) onto a platinum (Pt) surface using a solution of 5%, 10%, 15%cellulose acetate and 25% glutaraldehyde. The result of this research show that detection
limit of the sensor using cellulose acetate 5%, 10 % and 15 % are 10-7.7M, 1US.7M and 1(17.6M respectively. The results are approximatelyequal to 2.2 ppb-0.2 ppb, which means that this biosensor is very sensitive for determining carbamates pesticides residue. Detection limit of biosensors are comparable to that of the conventional instrument such as Gas Chromatography (GC) and High Pressure Liquid Chromatography (HPLC), i.e. 1.5 ppb and 2.0 ppb
respectively. The proposed electrochemical pesticide sensitivity test exhibited high sensitivity, desirable accuracy, low cost and simplified procedure.
Keywords: Biosensors, glutaraldehyde, cellulose acetate, immobilised, carbamate pesticides
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