The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion
ABSTRACT Ultraviolet 8 (UVB) radiation can act as immunosuppressant by inducing an epidermal Langerhans cells (LC) depletion which could be inhibited by topical sunscreen. Several kinds of sunscreens with various SPF (Sunscreen Protection Factor) are now available. The minimal SPF which able to inhi...
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| Format: | Article |
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[Yogyakarta] : Universitas Gadjah Mada
2010
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| _version_ | 1826033164209881088 |
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | ABSTRACT
Ultraviolet 8 (UVB) radiation can act as immunosuppressant by inducing an epidermal Langerhans cells (LC) depletion which could be inhibited by topical sunscreen. Several kinds of sunscreens with various SPF (Sunscreen Protection Factor) are now available. The minimal SPF which able to inhibit the immunosuppressive effect of UVB
among people with skin photo-type IV has not been established yet. The aim of this study is to determine the minimal SPF capable to inhibit UVB-induced immunosuppression. among people with skin photo-type IV. A simple experimental (post test only experimental) study was conducted among 5 people's circumsized foreskins with Fitzpatrick's skin photo-type IV. Each of them was divided into equal 5 pieces of 0.5 em'. Each of three pieces of skin was treated by sunscreen SPF 15, SPF 30, and SPF 50, a single piece of skin was treated with placebo, and all
of them then were treated by a single 100 mJ/cm' of UVB30 minutes later. A rest single piece of skin was used as control. After 24 hours of incubation in incubator of 37° C and 5% C02, all of them then was fixed by buffer formalin, blocked by paraffin, cut in 2 mm of thickness, and then stained with anti CD 1" antibody with AEC as chromogen and Mayer's hematoxylin as counterstaining. The number of LC was counted by Image J Analysis programmed and the mean of LCwere analyzed by Kruskal-Wallis test dan Mann-Whitney test. There were very significantly different of the mean number of LC between UVB placebo group and control group (p < 0.01).
Compared to the control group, mean number of LC among SPF 30 and SPF 50 treated groups were not
significantly different (p > 0.051. Sunscreen with SPF 15 had LC number lower than control group significantly (p < 0.05). The lowest SPFfor preventing UVB induced LC depletion among people with skin photo-type IVwas 30.
Key words: UVB- immunosupression - sunscreens - SPF -CD1"expression |
| first_indexed | 2024-03-13T19:05:51Z |
| format | Article |
| id | oai:generic.eprints.org:28119 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:05:51Z |
| publishDate | 2010 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:281192014-06-18T00:24:17Z https://repository.ugm.ac.id/28119/ The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion Perpustakaan UGM, i-lib Jurnal i-lib UGM ABSTRACT Ultraviolet 8 (UVB) radiation can act as immunosuppressant by inducing an epidermal Langerhans cells (LC) depletion which could be inhibited by topical sunscreen. Several kinds of sunscreens with various SPF (Sunscreen Protection Factor) are now available. The minimal SPF which able to inhibit the immunosuppressive effect of UVB among people with skin photo-type IV has not been established yet. The aim of this study is to determine the minimal SPF capable to inhibit UVB-induced immunosuppression. among people with skin photo-type IV. A simple experimental (post test only experimental) study was conducted among 5 people's circumsized foreskins with Fitzpatrick's skin photo-type IV. Each of them was divided into equal 5 pieces of 0.5 em'. Each of three pieces of skin was treated by sunscreen SPF 15, SPF 30, and SPF 50, a single piece of skin was treated with placebo, and all of them then were treated by a single 100 mJ/cm' of UVB30 minutes later. A rest single piece of skin was used as control. After 24 hours of incubation in incubator of 37° C and 5% C02, all of them then was fixed by buffer formalin, blocked by paraffin, cut in 2 mm of thickness, and then stained with anti CD 1" antibody with AEC as chromogen and Mayer's hematoxylin as counterstaining. The number of LC was counted by Image J Analysis programmed and the mean of LCwere analyzed by Kruskal-Wallis test dan Mann-Whitney test. There were very significantly different of the mean number of LC between UVB placebo group and control group (p < 0.01). Compared to the control group, mean number of LC among SPF 30 and SPF 50 treated groups were not significantly different (p > 0.051. Sunscreen with SPF 15 had LC number lower than control group significantly (p < 0.05). The lowest SPFfor preventing UVB induced LC depletion among people with skin photo-type IVwas 30. Key words: UVB- immunosupression - sunscreens - SPF -CD1"expression [Yogyakarta] : Universitas Gadjah Mada 2010 Article NonPeerReviewed Perpustakaan UGM, i-lib (2010) The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11182 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion |
| title | The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion |
| title_full | The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion |
| title_fullStr | The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion |
| title_full_unstemmed | The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion |
| title_short | The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion |
| title_sort | protective effect of sunscreens against ultraviolet b induced immunosuppression a study on langerhans cell depletion |
| topic | Jurnal i-lib UGM |
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