Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections
Although viruses are known to modify the free radical concentration in infected cells, the exact location and concentrations of such changes remain unknown. Although this information is important to understand the virus pathogenesis and design better anti-viral drugs or vaccines, obtaining it with...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/281681/1/Morita_KG.pdf |
| _version_ | 1797037474738864128 |
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| author | Wu, Kaiqi Vedelaar, Thea A. Damle, Viraj G. Morita, Arian Maugnaud, Julie Martin, Claudia Reyes San Zhang, Zue Pol, Denise P.I. Van der Ende_Metselaar, Heidi Rodenhuis-Zybert, Izabela Shirhagl, Romana |
| author_facet | Wu, Kaiqi Vedelaar, Thea A. Damle, Viraj G. Morita, Arian Maugnaud, Julie Martin, Claudia Reyes San Zhang, Zue Pol, Denise P.I. Van der Ende_Metselaar, Heidi Rodenhuis-Zybert, Izabela Shirhagl, Romana |
| author_sort | Wu, Kaiqi |
| collection | UGM |
| description | Although viruses are known to modify the free radical concentration in infected cells, the exact location and
concentrations of such changes remain unknown. Although this information is important to understand the virus pathogenesis and design better anti-viral drugs or vaccines, obtaining it with the conventional free radical/ROS
detection techniques is impossible. Here, we elucidate the utility of diamond magnetometry for studying the free
radical response of baby hamster kidney-21 cells upon Semliki Forest virus infection. Specifically, we optically
probe the alterations in free radical concentration near infectious viruses via measuring the spin–lattice relaxation (T1) of NV defect ensembles embedded in intracellular nanodiamonds. We performed measurements both at random locations as well as close to the virus entry by conjugating viruses to nanodiamond sensors. We observed alterations of T1, which represent the intracellular free radical concentration during the viral replication process. Moreover, relaxometry is also used to monitor real-time free radical variation during the early infectious process. |
| first_indexed | 2024-03-14T00:03:53Z |
| format | Article |
| id | oai:generic.eprints.org:281681 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:03:53Z |
| publishDate | 2022 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2816812023-11-10T09:27:23Z https://repository.ugm.ac.id/281681/ Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections Wu, Kaiqi Vedelaar, Thea A. Damle, Viraj G. Morita, Arian Maugnaud, Julie Martin, Claudia Reyes San Zhang, Zue Pol, Denise P.I. Van der Ende_Metselaar, Heidi Rodenhuis-Zybert, Izabela Shirhagl, Romana Dentistry Although viruses are known to modify the free radical concentration in infected cells, the exact location and concentrations of such changes remain unknown. Although this information is important to understand the virus pathogenesis and design better anti-viral drugs or vaccines, obtaining it with the conventional free radical/ROS detection techniques is impossible. Here, we elucidate the utility of diamond magnetometry for studying the free radical response of baby hamster kidney-21 cells upon Semliki Forest virus infection. Specifically, we optically probe the alterations in free radical concentration near infectious viruses via measuring the spin–lattice relaxation (T1) of NV defect ensembles embedded in intracellular nanodiamonds. We performed measurements both at random locations as well as close to the virus entry by conjugating viruses to nanodiamond sensors. We observed alterations of T1, which represent the intracellular free radical concentration during the viral replication process. Moreover, relaxometry is also used to monitor real-time free radical variation during the early infectious process. Elsevier 2022-03-12 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/281681/1/Morita_KG.pdf Wu, Kaiqi and Vedelaar, Thea A. and Damle, Viraj G. and Morita, Arian and Maugnaud, Julie and Martin, Claudia Reyes San and Zhang, Zue and Pol, Denise P.I. Van der and Ende_Metselaar, Heidi and Rodenhuis-Zybert, Izabela and Shirhagl, Romana (2022) Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections. Redox Biology, 52 (2022). pp. 1-11. ISSN 2213-2317 https://www.elsevier.com/locate/redox https://doi.org/10.1016/j.redox.2022.102279 |
| spellingShingle | Dentistry Wu, Kaiqi Vedelaar, Thea A. Damle, Viraj G. Morita, Arian Maugnaud, Julie Martin, Claudia Reyes San Zhang, Zue Pol, Denise P.I. Van der Ende_Metselaar, Heidi Rodenhuis-Zybert, Izabela Shirhagl, Romana Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections |
| title | Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections |
| title_full | Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections |
| title_fullStr | Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections |
| title_full_unstemmed | Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections |
| title_short | Applying NV center-based quantum sensing to study intracellular free radical response upon viral infections |
| title_sort | applying nv center based quantum sensing to study intracellular free radical response upon viral infections |
| topic | Dentistry |
| url | https://repository.ugm.ac.id/281681/1/Morita_KG.pdf |
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