Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression
Introduction: MicroRNAs (miRNAs) are short-sequence RNAs that regulate gene expression by targeting messenger RNAs (mRNAs). Recent studies reveal that miRNA-324-5p plays an important role in worsening the ovarian cancer prognosis when the expression is very high. This study aimed to develop a miRNA...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2022
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| Online Access: | https://repository.ugm.ac.id/281844/1/Chitosan%20nanoparticle-mediated%20effect%20of%20antimiRNA-324-5p%20on%20decreasing%20the%20ovarian%20cancer%20cell%20proliferation%20by%20regulation%20of%20GLI1%20expression.pdf |
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| author | Ysrafil, Ysrafil Astuti, Indwiani |
| author_facet | Ysrafil, Ysrafil Astuti, Indwiani |
| author_sort | Ysrafil, Ysrafil |
| collection | UGM |
| description | Introduction: MicroRNAs (miRNAs) are short-sequence RNAs that regulate gene expression by targeting messenger RNAs (mRNAs). Recent studies reveal that miRNA-324-5p plays an important role in worsening the ovarian cancer prognosis when the expression is very high. This study aimed to develop a miRNA targeted therapy by targeting the miRNA-324-5p function as a miRNA-324-5p inhibitor. Methods: Chitosan nanoparticles were used for antimiRNA-324-5p delivery into SKOV3 cell lines formulated by ionic gelation method. Antiproliferative effect of CS-NPs-antimiRNA was assessed by the MTT Assay. A mechanism study assessed the anticancer effect of the formula. In silico analysis used miRTar.Human and StarmiRDB combined with Genecard to predict the target genes of antimiR. Hawkdock web server was used to analyze protein-protein interactions that were further validated by quantitative polymerase chain reaction (qPCR). Results: The results of qPCR analysis showed endogenous miRNA-324-5p decreased after 24-hour transfection of antagonist miRNA. Furthermore, the MTT assay results showed that antimiRNA was able to inhibit SKOV3 cell proliferation (80 nM 68.13%, |
| first_indexed | 2024-03-14T00:04:04Z |
| format | Article |
| id | oai:generic.eprints.org:281844 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:04:04Z |
| publishDate | 2022 |
| publisher | Tabriz University of Medical Sciences |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2818442023-11-14T07:17:49Z https://repository.ugm.ac.id/281844/ Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression Ysrafil, Ysrafil Astuti, Indwiani Pharmacology and Pharmaceutical Sciences Introduction: MicroRNAs (miRNAs) are short-sequence RNAs that regulate gene expression by targeting messenger RNAs (mRNAs). Recent studies reveal that miRNA-324-5p plays an important role in worsening the ovarian cancer prognosis when the expression is very high. This study aimed to develop a miRNA targeted therapy by targeting the miRNA-324-5p function as a miRNA-324-5p inhibitor. Methods: Chitosan nanoparticles were used for antimiRNA-324-5p delivery into SKOV3 cell lines formulated by ionic gelation method. Antiproliferative effect of CS-NPs-antimiRNA was assessed by the MTT Assay. A mechanism study assessed the anticancer effect of the formula. In silico analysis used miRTar.Human and StarmiRDB combined with Genecard to predict the target genes of antimiR. Hawkdock web server was used to analyze protein-protein interactions that were further validated by quantitative polymerase chain reaction (qPCR). Results: The results of qPCR analysis showed endogenous miRNA-324-5p decreased after 24-hour transfection of antagonist miRNA. Furthermore, the MTT assay results showed that antimiRNA was able to inhibit SKOV3 cell proliferation (80 nM 68.13%, Tabriz University of Medical Sciences 2022-05 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/281844/1/Chitosan%20nanoparticle-mediated%20effect%20of%20antimiRNA-324-5p%20on%20decreasing%20the%20ovarian%20cancer%20cell%20proliferation%20by%20regulation%20of%20GLI1%20expression.pdf Ysrafil, Ysrafil and Astuti, Indwiani (2022) Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression. BioImpacts, 12 (3). pp. 195-202. ISSN 2228-5652 https://bi.tbzmed.ac.ir/Article/bi-22119 https://doi.org/10.34172/bi.2021.22119 |
| spellingShingle | Pharmacology and Pharmaceutical Sciences Ysrafil, Ysrafil Astuti, Indwiani Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression |
| title | Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression |
| title_full | Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression |
| title_fullStr | Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression |
| title_full_unstemmed | Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression |
| title_short | Chitosan nanoparticle-mediated effect of antimiRNA-324-5p on decreasing the ovarian cancer cell proliferation by regulation of GLI1 expression |
| title_sort | chitosan nanoparticle mediated effect of antimirna 324 5p on decreasing the ovarian cancer cell proliferation by regulation of gli1 expression |
| topic | Pharmacology and Pharmaceutical Sciences |
| url | https://repository.ugm.ac.id/281844/1/Chitosan%20nanoparticle-mediated%20effect%20of%20antimiRNA-324-5p%20on%20decreasing%20the%20ovarian%20cancer%20cell%20proliferation%20by%20regulation%20of%20GLI1%20expression.pdf |
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