Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats
Uncontrolled diabetes mellitus (DM) has been linked to depression and lipid metabolism impairment. Serotonin (5-HT) has been known to improve lipid metabolism and affect mood. The 5-Hydroxytryptophan (5-HTP) in banana peel flour (BPF) is a precursor to synthesize 5-HT in the body, which is linked to...
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Department of Biology, IPB University
2022
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Online Access: | https://repository.ugm.ac.id/281846/1/Meliala_KKMK.pdf |
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author | Meliala, Andreanyta Narwidina, Paramita Nurrahma, Herlin Ajeng Supriyanto, Irwan |
author_facet | Meliala, Andreanyta Narwidina, Paramita Nurrahma, Herlin Ajeng Supriyanto, Irwan |
author_sort | Meliala, Andreanyta |
collection | UGM |
description | Uncontrolled diabetes mellitus (DM) has been linked to depression and lipid metabolism impairment. Serotonin (5-HT) has been known to improve lipid metabolism and affect mood. The 5-Hydroxytryptophan (5-HTP) in banana peel flour (BPF) is a precursor to synthesize 5-HT in the body, which is linked to initiating liver regeneration via HTR2B receptors. The impact of 5-HTP in the diet on HTR2B receptor expression has yet to be thoroughly investigated. We aimed to elucidate
the correlation between serum lipid profile and immobility time in Tail Suspension Test (TST) as depressive-like behavior and compare the expression of HTR2B receptor in healthy and diabetic rats. Male Wistar (Rattus norvegicus) rats were assigned to the control and treatment groups in a DM model with streptozotocin (60 mg/kg) injected intraperitoneally, then either fed standard diet or BPF 10%
supplemented standard diet for 21 days. Immunohistochemical staining was used to examine the expression of 5-HT, HTR2B receptors. We determined that diabetic rats fed the standard diet supplemented 10% BPF group showed significantly lower
concentrations of total cholesterol and triglycerides compared to diabetic rats fed a standard diet and it was positively correlated between total cholesterol and LDL
with the duration of immobility time in TST. The average immunoreactivity score in diabetic rats fed 10% BPF was the highest among the other groups, indicating that the available BPF dose is sufficient for HTR2B activation, which will support the liver cell regeneration process, and should be investigated further. |
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format | Article |
id | oai:generic.eprints.org:281846 |
institution | Universiti Gadjah Mada |
language | English |
last_indexed | 2024-03-14T00:04:04Z |
publishDate | 2022 |
publisher | Department of Biology, IPB University |
record_format | dspace |
spelling | oai:generic.eprints.org:2818462023-11-14T07:33:13Z https://repository.ugm.ac.id/281846/ Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats Meliala, Andreanyta Narwidina, Paramita Nurrahma, Herlin Ajeng Supriyanto, Irwan Clinical Sciences Uncontrolled diabetes mellitus (DM) has been linked to depression and lipid metabolism impairment. Serotonin (5-HT) has been known to improve lipid metabolism and affect mood. The 5-Hydroxytryptophan (5-HTP) in banana peel flour (BPF) is a precursor to synthesize 5-HT in the body, which is linked to initiating liver regeneration via HTR2B receptors. The impact of 5-HTP in the diet on HTR2B receptor expression has yet to be thoroughly investigated. We aimed to elucidate the correlation between serum lipid profile and immobility time in Tail Suspension Test (TST) as depressive-like behavior and compare the expression of HTR2B receptor in healthy and diabetic rats. Male Wistar (Rattus norvegicus) rats were assigned to the control and treatment groups in a DM model with streptozotocin (60 mg/kg) injected intraperitoneally, then either fed standard diet or BPF 10% supplemented standard diet for 21 days. Immunohistochemical staining was used to examine the expression of 5-HT, HTR2B receptors. We determined that diabetic rats fed the standard diet supplemented 10% BPF group showed significantly lower concentrations of total cholesterol and triglycerides compared to diabetic rats fed a standard diet and it was positively correlated between total cholesterol and LDL with the duration of immobility time in TST. The average immunoreactivity score in diabetic rats fed 10% BPF was the highest among the other groups, indicating that the available BPF dose is sufficient for HTR2B activation, which will support the liver cell regeneration process, and should be investigated further. Department of Biology, IPB University 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/281846/1/Meliala_KKMK.pdf Meliala, Andreanyta and Narwidina, Paramita and Nurrahma, Herlin Ajeng and Supriyanto, Irwan (2022) Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats. HAYATI Journal of Biosciences, 29 (3). pp. 279-287. ISSN 2086-4094 https://journal.ipb.ac.id/index.php/hayati/article/view/37397 https://doi.org/10.4308/hjb.29.3.279-287 |
spellingShingle | Clinical Sciences Meliala, Andreanyta Narwidina, Paramita Nurrahma, Herlin Ajeng Supriyanto, Irwan Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B Receptor Expression in the Liver Diabetic Rats |
title | Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B
Receptor Expression in the Liver Diabetic Rats |
title_full | Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B
Receptor Expression in the Liver Diabetic Rats |
title_fullStr | Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B
Receptor Expression in the Liver Diabetic Rats |
title_full_unstemmed | Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B
Receptor Expression in the Liver Diabetic Rats |
title_short | Banana (Musa balbisiana Colla) Peels Flour Modulates HTR2B
Receptor Expression in the Liver Diabetic Rats |
title_sort | banana musa balbisiana colla peels flour modulates htr2b receptor expression in the liver diabetic rats |
topic | Clinical Sciences |
url | https://repository.ugm.ac.id/281846/1/Meliala_KKMK.pdf |
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