The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks

Prolactin (PRL) affects egg production in duck as it induces broodiness and promotes follicles development. The objective of this study was to investigate the association between polymorphism of PRL gene and egg production traits in Alabio and Mojosari Ducks. Genomic DNA were isolated from 111 blood...

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Main Authors: Damayanti, I., Sudaryati, S., Maharani, D.
Format: Article
Language:English
Published: 2022
Subjects:
Online Access:https://repository.ugm.ac.id/281932/1/45616-153786-1-PB.pdf
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author Damayanti, I.
Sudaryati, S.
Maharani, D.
author_facet Damayanti, I.
Sudaryati, S.
Maharani, D.
author_sort Damayanti, I.
collection UGM
description Prolactin (PRL) affects egg production in duck as it induces broodiness and promotes follicles development. The objective of this study was to investigate the association between polymorphism of PRL gene and egg production traits in Alabio and Mojosari Ducks. Genomic DNA were isolated from 111 blood samples (51 Alabio and 60 Mojosari). PCR and sequencing were performed to identify polymorphisms and genotype of the animals. Data recording of the ducks including body weight at 16 weeks of age and at first egg, average weight of three first eggs laid and egg number laid up to 3, 6 and 12 months production were collected. Data were analyzed using independent sample t-Test. As a result, two single nucleotide polymorphisms (SNP) were detected in intron 4. Both SNP C-5796A and SNP T-5817C were found to have association with egg number laid up to three months (P<0, 005). The CA/TC genotype had higher egg number than CC/TT. These suggested that SNP C-5796A and SNP T-5817C could be potential markers for marker assisted selection to increase egg number in duck. © 2022 Diponegoro University. All rights reserved.
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spelling oai:generic.eprints.org:2819322023-11-15T05:24:25Z https://repository.ugm.ac.id/281932/ The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks Damayanti, I. Sudaryati, S. Maharani, D. Animal Production Animal Breeding Prolactin (PRL) affects egg production in duck as it induces broodiness and promotes follicles development. The objective of this study was to investigate the association between polymorphism of PRL gene and egg production traits in Alabio and Mojosari Ducks. Genomic DNA were isolated from 111 blood samples (51 Alabio and 60 Mojosari). PCR and sequencing were performed to identify polymorphisms and genotype of the animals. Data recording of the ducks including body weight at 16 weeks of age and at first egg, average weight of three first eggs laid and egg number laid up to 3, 6 and 12 months production were collected. Data were analyzed using independent sample t-Test. As a result, two single nucleotide polymorphisms (SNP) were detected in intron 4. Both SNP C-5796A and SNP T-5817C were found to have association with egg number laid up to three months (P<0, 005). The CA/TC genotype had higher egg number than CC/TT. These suggested that SNP C-5796A and SNP T-5817C could be potential markers for marker assisted selection to increase egg number in duck. © 2022 Diponegoro University. All rights reserved. 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/281932/1/45616-153786-1-PB.pdf Damayanti, I. and Sudaryati, S. and Maharani, D. (2022) The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks. Journal of the Indonesian Tropical Animal Agriculture, 47 (3). 184 – 191. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138668810&doi=10.14710%2fjitaa.47.3.184-191&partnerID=40&md5=0d38bbc3b0622af9f11024d79391f9b3
spellingShingle Animal Production
Animal Breeding
Damayanti, I.
Sudaryati, S.
Maharani, D.
The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks
title The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks
title_full The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks
title_fullStr The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks
title_full_unstemmed The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks
title_short The association of prolactin gene polymorphism with egg production traits in Alabio and Mojosari ducks
title_sort association of prolactin gene polymorphism with egg production traits in alabio and mojosari ducks
topic Animal Production
Animal Breeding
url https://repository.ugm.ac.id/281932/1/45616-153786-1-PB.pdf
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