Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells
Senescence-induced therapy has been improved to increase its cytotoxicity and reduce the resistance of breast cancer cells to chemotherapy agents. An example of a potential senescence-inducing agent is black cumin oil (BCO) because one of its major compounds, α-pinene, can induce senescent cells. Th...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Malaysian Society of Applied Biology
2022
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/282006/1/Ahlina_FA.pdf |
| _version_ | 1826050467991388160 |
|---|---|
| author | Ahlina, Faradiba Nur Anggriani, Lisyaratih Salsabila, Irfani Aura Jenie, Riris Istighfari |
| author_facet | Ahlina, Faradiba Nur Anggriani, Lisyaratih Salsabila, Irfani Aura Jenie, Riris Istighfari |
| author_sort | Ahlina, Faradiba Nur |
| collection | UGM |
| description | Senescence-induced therapy has been improved to increase its cytotoxicity and reduce the resistance of breast cancer cells to chemotherapy agents. An example of a potential senescence-inducing agent is black cumin oil (BCO) because one of its major compounds, α-pinene, can induce senescent cells. This study aims to explore the senescence-inducing activity of BCO in HER2-overexpressing breast cancer cells (MCF7/HER2). The yield obtained from hydro-distillation of BCO was 0.54%, and the main compounds were p-cymene (48.03%), dihydrocarveol (11.39%), and α-pinene (11.29%). BCO exhibited a moderate cytotoxicity profile indicated by IC50, which was >200 μg/mL in both cell lines. In combination with doxorubicin, BCO did not increase the cytotoxicity of doxorubicin. Moreover, BCO induced senescence by increasing 3% of the senescent cells compared
with that of the untreated cells. A combination of BCO and doxorubicin increased the senescent cells by 3%–7% compared
with doxorubicin alone. Therefore, the moderate cytotoxicity of BCO could be beneficial to the application of BCO as a
chemotherapeutic adjuvant which increases cancer cells senescent and consequently inhibits cell proliferation. |
| first_indexed | 2024-03-14T00:04:27Z |
| format | Article |
| id | oai:generic.eprints.org:282006 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:04:27Z |
| publishDate | 2022 |
| publisher | The Malaysian Society of Applied Biology |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2820062023-11-16T02:25:32Z https://repository.ugm.ac.id/282006/ Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells Ahlina, Faradiba Nur Anggriani, Lisyaratih Salsabila, Irfani Aura Jenie, Riris Istighfari Pharmaceutical Sciences Senescence-induced therapy has been improved to increase its cytotoxicity and reduce the resistance of breast cancer cells to chemotherapy agents. An example of a potential senescence-inducing agent is black cumin oil (BCO) because one of its major compounds, α-pinene, can induce senescent cells. This study aims to explore the senescence-inducing activity of BCO in HER2-overexpressing breast cancer cells (MCF7/HER2). The yield obtained from hydro-distillation of BCO was 0.54%, and the main compounds were p-cymene (48.03%), dihydrocarveol (11.39%), and α-pinene (11.29%). BCO exhibited a moderate cytotoxicity profile indicated by IC50, which was >200 μg/mL in both cell lines. In combination with doxorubicin, BCO did not increase the cytotoxicity of doxorubicin. Moreover, BCO induced senescence by increasing 3% of the senescent cells compared with that of the untreated cells. A combination of BCO and doxorubicin increased the senescent cells by 3%–7% compared with doxorubicin alone. Therefore, the moderate cytotoxicity of BCO could be beneficial to the application of BCO as a chemotherapeutic adjuvant which increases cancer cells senescent and consequently inhibits cell proliferation. The Malaysian Society of Applied Biology 2022-03-31 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/282006/1/Ahlina_FA.pdf Ahlina, Faradiba Nur and Anggriani, Lisyaratih and Salsabila, Irfani Aura and Jenie, Riris Istighfari (2022) Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells. Malays. Appl. Biol., 51 (1). pp. 91-98. ISSN 2462-151X https://jms.mabjournal.com/index.php/mab/article/view/2008 https://doi.org/10.55230/mabjournal.v51i1.2008 |
| spellingShingle | Pharmaceutical Sciences Ahlina, Faradiba Nur Anggriani, Lisyaratih Salsabila, Irfani Aura Jenie, Riris Istighfari Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells |
| title | Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells |
| title_full | Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells |
| title_fullStr | Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells |
| title_full_unstemmed | Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells |
| title_short | Bioactivity of Black Cumin Oil on the Senescence of Her-2-Overexpressing Breast Cancer Cells |
| title_sort | bioactivity of black cumin oil on the senescence of her 2 overexpressing breast cancer cells |
| topic | Pharmaceutical Sciences |
| url | https://repository.ugm.ac.id/282006/1/Ahlina_FA.pdf |
| work_keys_str_mv | AT ahlinafaradibanur bioactivityofblackcuminoilonthesenescenceofher2overexpressingbreastcancercells AT anggrianilisyaratih bioactivityofblackcuminoilonthesenescenceofher2overexpressingbreastcancercells AT salsabilairfaniaura bioactivityofblackcuminoilonthesenescenceofher2overexpressingbreastcancercells AT jenieririsistighfari bioactivityofblackcuminoilonthesenescenceofher2overexpressingbreastcancercells |