Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis
Polyhydroxybutyrate (PHB) is a member of the Polyhydroxyalkanoates (PHAs) family, which is generally produced through microbial biosynthesis. The synthetic biology tools are applied to transform PHB genes (PhaA, PhaB and PhaC) into an oleaginous yeast (Rhodotorula glutinis) genome. Western blot anal...
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| Format: | Article |
| Language: | English |
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Elsevier Ltd.
2022
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| Online Access: | https://repository.ugm.ac.id/282046/1/Aristya%20et%20al%20-%202022%20-%20Polyhydroxybutyrate%20%28PHB%29%20production%20from%20crude.pdf |
| _version_ | 1797037523049906176 |
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| author | Aristya, Ganies Riza Lin, Yu-Ju Chang, Jo-Shu Chang, Jui-Jen Yen, Hong-Wei |
| author_facet | Aristya, Ganies Riza Lin, Yu-Ju Chang, Jo-Shu Chang, Jui-Jen Yen, Hong-Wei |
| author_sort | Aristya, Ganies Riza |
| collection | UGM |
| description | Polyhydroxybutyrate (PHB) is a member of the Polyhydroxyalkanoates (PHAs) family, which is generally produced through microbial biosynthesis. The synthetic biology tools are applied to transform PHB genes (PhaA, PhaB and PhaC) into an oleaginous yeast (Rhodotorula glutinis) genome. Western blot analysis confirms the expression of PhaA, PhaB and PhaC in the candidate transformants. The strain of R. glutinis #100–29 was selected to be the best PHB production candidate according to the shaker results. As compared to glucose, crude glycerol was known to be a suitable carbon source for R. glutinis cultivation. The PHB concentration, yield and content obtained in a 5-L fermenter using 30 g/L of crude glycerol were 2.87 g/L, 95.53 mg/g and 62 respectively. To the best of our knowledge, these should be the highest numbers obtained in yeast for PHB production, which indicates that strain #100–29 could be a potential PHB producer from crude glycerol. © 2022 Elsevier Ltd |
| first_indexed | 2024-03-14T00:04:34Z |
| format | Article |
| id | oai:generic.eprints.org:282046 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:04:34Z |
| publishDate | 2022 |
| publisher | Elsevier Ltd. |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2820462023-12-01T06:30:54Z https://repository.ugm.ac.id/282046/ Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis Aristya, Ganies Riza Lin, Yu-Ju Chang, Jo-Shu Chang, Jui-Jen Yen, Hong-Wei Other Biological Sciences Biological Sciences not elsewhere classified Polyhydroxybutyrate (PHB) is a member of the Polyhydroxyalkanoates (PHAs) family, which is generally produced through microbial biosynthesis. The synthetic biology tools are applied to transform PHB genes (PhaA, PhaB and PhaC) into an oleaginous yeast (Rhodotorula glutinis) genome. Western blot analysis confirms the expression of PhaA, PhaB and PhaC in the candidate transformants. The strain of R. glutinis #100–29 was selected to be the best PHB production candidate according to the shaker results. As compared to glucose, crude glycerol was known to be a suitable carbon source for R. glutinis cultivation. The PHB concentration, yield and content obtained in a 5-L fermenter using 30 g/L of crude glycerol were 2.87 g/L, 95.53 mg/g and 62 respectively. To the best of our knowledge, these should be the highest numbers obtained in yeast for PHB production, which indicates that strain #100–29 could be a potential PHB producer from crude glycerol. © 2022 Elsevier Ltd Elsevier Ltd. 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/282046/1/Aristya%20et%20al%20-%202022%20-%20Polyhydroxybutyrate%20%28PHB%29%20production%20from%20crude.pdf Aristya, Ganies Riza and Lin, Yu-Ju and Chang, Jo-Shu and Chang, Jui-Jen and Yen, Hong-Wei (2022) Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis. Bioresource Technology Reports, 18. pp. 1-9. ISSN 2589-014X https://www.sciencedirect.com/science/article/pii/S2589014X22001050 |
| spellingShingle | Other Biological Sciences Biological Sciences not elsewhere classified Aristya, Ganies Riza Lin, Yu-Ju Chang, Jo-Shu Chang, Jui-Jen Yen, Hong-Wei Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis |
| title | Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis |
| title_full | Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis |
| title_fullStr | Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis |
| title_full_unstemmed | Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis |
| title_short | Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis |
| title_sort | polyhydroxybutyrate phb production from crude glycerol by genetic engineering of rhodotorula glutinis |
| topic | Other Biological Sciences Biological Sciences not elsewhere classified |
| url | https://repository.ugm.ac.id/282046/1/Aristya%20et%20al%20-%202022%20-%20Polyhydroxybutyrate%20%28PHB%29%20production%20from%20crude.pdf |
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