Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha
Clitoria ternatea, with an alternative name, Butterfly pea, is increasingly being explored for medical purposes and the development of a wide range of processed products. This study aimed to incorporate Butterfly pea into an innovative probiotic drink through a symbiotic culture of bacteria and yeas...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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ScienceDirect
2022
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| Online Access: | https://repository.ugm.ac.id/282464/1/2.pdf |
| _version_ | 1826050528694501376 |
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| author | Permatasari, Happy Kurnia Nurkolis, Fahrul Gunawan, William Ben Yusuf, Vincentius Mario Yusuf, Muhammad Kusuma, Rio Jati Sabrina, Nindy Muharram, Farizal Rizky Taslim, Nurpudji Astuti Mayulu, Nelly Batubara, Siti Chairiyah Samtiya, Mrinal Hardinsyah, Hardinsyah Tsopmo, Apollinaire |
| author_facet | Permatasari, Happy Kurnia Nurkolis, Fahrul Gunawan, William Ben Yusuf, Vincentius Mario Yusuf, Muhammad Kusuma, Rio Jati Sabrina, Nindy Muharram, Farizal Rizky Taslim, Nurpudji Astuti Mayulu, Nelly Batubara, Siti Chairiyah Samtiya, Mrinal Hardinsyah, Hardinsyah Tsopmo, Apollinaire |
| author_sort | Permatasari, Happy Kurnia |
| collection | UGM |
| description | Clitoria ternatea, with an alternative name, Butterfly pea, is increasingly being explored for medical purposes and the development of a wide range of processed products. This study aimed to incorporate Butterfly pea into an innovative probiotic drink through a symbiotic culture of bacteria and yeast (SCOBY) fermentation and to evaluate the biological activity. The benefits of the drink, referred to as butterfly pea flower kombucha (KBPF) was determined in vitro and in metabolically disorder mice that receive a diet rich in cholesterol and fat (CFED). Forty white male were categorized into four groups, i.e., A = Control/Normal Diet; B = CFED alone; C = CFED + KBPF 65 mg/kg BW (Body Weight); D = CFED + KBPF 130 mg/kg BW, and then sacrificed after 6 weeks of intervention. Seventy-nine secondary metabolite compounds were successfully identified in KBPF using LC-HRMS. In vitro studies showed the potential activity of KBPF in inhibiting not only ABTS, but also lipid (lipase) and carbohydrate (α-amylase, α-glucosidase) hydrolyzing enzymes to levels similar to acarbose control at 50–250 μg/mL. In the in vivo study, the administration of KBPF (130 mg/kg BW) significantly alleviated metabolic disorders caused by high-fat diet. Specifically, lipid profile (HDL, LDL, TC, TG), blood glucose, markers of oxidative stress (SOD liver), metabolic enzymes (lipase, amylase), and markers of inflammation (PGC-1α, TNF-α, and IL-10) were in most cases restored to normal values. Additionally, the gut microbiota community analysis showed that KBPF has a positive effect (p = 0.01) on both the Bacteroidetes phylum and the Firmicutes phylum. The new KBPF drink is a promising therapeutic functional food for preventing metabolic diseases. |
| first_indexed | 2024-03-14T00:05:25Z |
| format | Article |
| id | oai:generic.eprints.org:282464 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:05:25Z |
| publishDate | 2022 |
| publisher | ScienceDirect |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2824642023-11-16T03:58:23Z https://repository.ugm.ac.id/282464/ Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha Permatasari, Happy Kurnia Nurkolis, Fahrul Gunawan, William Ben Yusuf, Vincentius Mario Yusuf, Muhammad Kusuma, Rio Jati Sabrina, Nindy Muharram, Farizal Rizky Taslim, Nurpudji Astuti Mayulu, Nelly Batubara, Siti Chairiyah Samtiya, Mrinal Hardinsyah, Hardinsyah Tsopmo, Apollinaire Medical Biochemistry - Lipids Clitoria ternatea, with an alternative name, Butterfly pea, is increasingly being explored for medical purposes and the development of a wide range of processed products. This study aimed to incorporate Butterfly pea into an innovative probiotic drink through a symbiotic culture of bacteria and yeast (SCOBY) fermentation and to evaluate the biological activity. The benefits of the drink, referred to as butterfly pea flower kombucha (KBPF) was determined in vitro and in metabolically disorder mice that receive a diet rich in cholesterol and fat (CFED). Forty white male were categorized into four groups, i.e., A = Control/Normal Diet; B = CFED alone; C = CFED + KBPF 65 mg/kg BW (Body Weight); D = CFED + KBPF 130 mg/kg BW, and then sacrificed after 6 weeks of intervention. Seventy-nine secondary metabolite compounds were successfully identified in KBPF using LC-HRMS. In vitro studies showed the potential activity of KBPF in inhibiting not only ABTS, but also lipid (lipase) and carbohydrate (α-amylase, α-glucosidase) hydrolyzing enzymes to levels similar to acarbose control at 50–250 μg/mL. In the in vivo study, the administration of KBPF (130 mg/kg BW) significantly alleviated metabolic disorders caused by high-fat diet. Specifically, lipid profile (HDL, LDL, TC, TG), blood glucose, markers of oxidative stress (SOD liver), metabolic enzymes (lipase, amylase), and markers of inflammation (PGC-1α, TNF-α, and IL-10) were in most cases restored to normal values. Additionally, the gut microbiota community analysis showed that KBPF has a positive effect (p = 0.01) on both the Bacteroidetes phylum and the Firmicutes phylum. The new KBPF drink is a promising therapeutic functional food for preventing metabolic diseases. ScienceDirect 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/282464/1/2.pdf Permatasari, Happy Kurnia and Nurkolis, Fahrul and Gunawan, William Ben and Yusuf, Vincentius Mario and Yusuf, Muhammad and Kusuma, Rio Jati and Sabrina, Nindy and Muharram, Farizal Rizky and Taslim, Nurpudji Astuti and Mayulu, Nelly and Batubara, Siti Chairiyah and Samtiya, Mrinal and Hardinsyah, Hardinsyah and Tsopmo, Apollinaire (2022) Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha. Current Research in Food Science, 5. pp. 1251-1265. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85136314454&doi=10.1016%2fj.crfs.2022.08.005&partnerID=40&md5=bc0a69e1d9e8f42e93bc3cd8bb828eeb 10.1016/j.crfs.2022.08.005 |
| spellingShingle | Medical Biochemistry - Lipids Permatasari, Happy Kurnia Nurkolis, Fahrul Gunawan, William Ben Yusuf, Vincentius Mario Yusuf, Muhammad Kusuma, Rio Jati Sabrina, Nindy Muharram, Farizal Rizky Taslim, Nurpudji Astuti Mayulu, Nelly Batubara, Siti Chairiyah Samtiya, Mrinal Hardinsyah, Hardinsyah Tsopmo, Apollinaire Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| title | Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| title_full | Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| title_fullStr | Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| title_full_unstemmed | Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| title_short | Modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| title_sort | modulation of gut microbiota and markers of metabolic syndrome in mice on cholesterol and fat enriched diet by butterfly pea flower kombucha |
| topic | Medical Biochemistry - Lipids |
| url | https://repository.ugm.ac.id/282464/1/2.pdf |
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