Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression
Staphylococcus aureus (S. aureus) is frequently detected in the skin of patients with atopic dermatitis (AD). AD skin-derived strains of S. aureus (AD strain) are selectively internalized into keratinocytes (HaCaT cells) compared to standard strains. However, the mechanism of AD strain internalizati...
| Main Authors: | , , , , , , , , |
|---|---|
| 格式: | 文件 |
| 出版: |
John Wiley and Sons Inc
2022
|
| 主题: |
| _version_ | 1826050531683991552 |
|---|---|
| author | Miyake, Ryu Iwamoto, Kazumasa Sakai, Norio Matsunae, Kyoka Aziz, Fatkhanuddin Sugai, Motoyuki Takahagi, Shunsuke Tanaka, Akio Hide, Michihiro |
| author_facet | Miyake, Ryu Iwamoto, Kazumasa Sakai, Norio Matsunae, Kyoka Aziz, Fatkhanuddin Sugai, Motoyuki Takahagi, Shunsuke Tanaka, Akio Hide, Michihiro |
| author_sort | Miyake, Ryu |
| collection | UGM |
| description | Staphylococcus aureus (S. aureus) is frequently detected in the skin of patients with atopic dermatitis (AD). AD skin-derived strains of S. aureus (AD strain) are selectively internalized into keratinocytes (HaCaT cells) compared to standard strains. However, the mechanism of AD strain internalization by keratinocytes and effect of the skin environment on internalization remain unclear. HaCaT cells were exposed to heat-killed AD or standard strains of fluorescently labeled S. aureus, with or without interferon (IFN)-γ, interleukin (IL)-4, and IL-13 cytokines, for 24 h. Filaggrin and fibronectin expression in HaCaT cells was knocked down using small interfering RNA. The amount of internalized S. aureus was evaluated using a cell imaging system. The effects of INF-γ, IL-4, and S. aureus exposure on mRNA expression in HaCaT cells were analyzed using single-cell RNA sequencing. AD strains adhered to HaCaT cells in approximately 15 min and were increasingly internalized for up to 3 h (2361 ± 467 spots/100 cells, mean ± SD), whereas the standard strain was not (991 ± 71 spots/100 cells). In the presence of IFN-γ, both the number of internalized strains and fibronectin expression significantly decreased compared to in the control, whereas Th2 cytokines had no significant effects. The number of internalized AD strains was significantly higher in filaggrin knockdown and lower in fibronectin knockdown HaCaT cells compared to in the control. RNA sequencing revealed that IFN-γ decreased both fibronectin and filaggrin expression. Keratinocyte internalization of the AD strain may be predominantly mediated by the INF-γ-fibronectin pathway and partially regulated by filaggrin expression. |
| first_indexed | 2024-03-14T00:05:27Z |
| format | Article |
| id | oai:generic.eprints.org:282657 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-14T00:05:27Z |
| publishDate | 2022 |
| publisher | John Wiley and Sons Inc |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2826572023-11-16T07:05:37Z https://repository.ugm.ac.id/282657/ Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression Miyake, Ryu Iwamoto, Kazumasa Sakai, Norio Matsunae, Kyoka Aziz, Fatkhanuddin Sugai, Motoyuki Takahagi, Shunsuke Tanaka, Akio Hide, Michihiro Biological Sciences Staphylococcus aureus (S. aureus) is frequently detected in the skin of patients with atopic dermatitis (AD). AD skin-derived strains of S. aureus (AD strain) are selectively internalized into keratinocytes (HaCaT cells) compared to standard strains. However, the mechanism of AD strain internalization by keratinocytes and effect of the skin environment on internalization remain unclear. HaCaT cells were exposed to heat-killed AD or standard strains of fluorescently labeled S. aureus, with or without interferon (IFN)-γ, interleukin (IL)-4, and IL-13 cytokines, for 24 h. Filaggrin and fibronectin expression in HaCaT cells was knocked down using small interfering RNA. The amount of internalized S. aureus was evaluated using a cell imaging system. The effects of INF-γ, IL-4, and S. aureus exposure on mRNA expression in HaCaT cells were analyzed using single-cell RNA sequencing. AD strains adhered to HaCaT cells in approximately 15 min and were increasingly internalized for up to 3 h (2361 ± 467 spots/100 cells, mean ± SD), whereas the standard strain was not (991 ± 71 spots/100 cells). In the presence of IFN-γ, both the number of internalized strains and fibronectin expression significantly decreased compared to in the control, whereas Th2 cytokines had no significant effects. The number of internalized AD strains was significantly higher in filaggrin knockdown and lower in fibronectin knockdown HaCaT cells compared to in the control. RNA sequencing revealed that IFN-γ decreased both fibronectin and filaggrin expression. Keratinocyte internalization of the AD strain may be predominantly mediated by the INF-γ-fibronectin pathway and partially regulated by filaggrin expression. John Wiley and Sons Inc 2022 Article PeerReviewed Miyake, Ryu and Iwamoto, Kazumasa and Sakai, Norio and Matsunae, Kyoka and Aziz, Fatkhanuddin and Sugai, Motoyuki and Takahagi, Shunsuke and Tanaka, Akio and Hide, Michihiro (2022) Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression. Journal of Dermatology, 49 (11). 1148 – 1157. ISSN 03852407 |
| spellingShingle | Biological Sciences Miyake, Ryu Iwamoto, Kazumasa Sakai, Norio Matsunae, Kyoka Aziz, Fatkhanuddin Sugai, Motoyuki Takahagi, Shunsuke Tanaka, Akio Hide, Michihiro Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression |
| title | Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression |
| title_full | Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression |
| title_fullStr | Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression |
| title_full_unstemmed | Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression |
| title_short | Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon–fibronectin pathway and filaggrin expression |
| title_sort | uptake of staphylococcus aureus by keratinocytes is reduced by interferon fibronectin pathway and filaggrin expression |
| topic | Biological Sciences |
| work_keys_str_mv | AT miyakeryu uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT iwamotokazumasa uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT sakainorio uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT matsunaekyoka uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT azizfatkhanuddin uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT sugaimotoyuki uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT takahagishunsuke uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT tanakaakio uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression AT hidemichihiro uptakeofstaphylococcusaureusbykeratinocytesisreducedbyinterferonfibronectinpathwayandfilaggrinexpression |