Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host
Aedes aegypti as a novel host. Biodiversitas 23: 2321-2328. Wolbachia naturally presents in a large number of insects and other arthropod species. The Wolbachia strain wMel from Drosophila melanogaster has been stably transinfected into Aedes aegypti where it stops the mosquito host from being infec...
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| Format: | Article |
| Language: | English |
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Society for Indonesian Biodiversity
2022
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| Online Access: | https://repository.ugm.ac.id/282740/1/8.pdf |
| _version_ | 1826050545750638592 |
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| author | Rovik, Anwar Daniwijaya, Edwin Widyanto Supriyati, Endah Rahayu,, Ayu Kumalawati, Dian Aruni Saraswati, Utari Handayaningsih, Anastasia Ev Rachman, Mifta Pratiwi Oktriani, Risky Kurniasari, Irianti Candrasari, Damiana Sapta Nurhayati, Indah Sholeh, Rizki Arianto, Budi Tantowijoyo, Warsito Ahmad, Riris Andono Utarini, Adi Arguni, Eggi |
| author_facet | Rovik, Anwar Daniwijaya, Edwin Widyanto Supriyati, Endah Rahayu,, Ayu Kumalawati, Dian Aruni Saraswati, Utari Handayaningsih, Anastasia Ev Rachman, Mifta Pratiwi Oktriani, Risky Kurniasari, Irianti Candrasari, Damiana Sapta Nurhayati, Indah Sholeh, Rizki Arianto, Budi Tantowijoyo, Warsito Ahmad, Riris Andono Utarini, Adi Arguni, Eggi |
| author_sort | Rovik, Anwar |
| collection | UGM |
| description | Aedes aegypti as a novel host. Biodiversitas 23: 2321-2328. Wolbachia naturally presents in a large number of insects and other arthropod species. The Wolbachia strain wMel from Drosophila melanogaster has been stably transinfected into Aedes aegypti where it stops the mosquito host from being infected with medically important arbovirus like dengue. Consequently, Ae. aegypti infected with wMel have been released in Indonesia as a public health intervention against dengue. This study genetically compared wMel from Yogya field-caught D. melanogaster and the wMel in stably transfected Ae. aegypti used for field releases in Yogyakarta, Indonesia. The genetic similarity between wMel Wolbachia was evaluated by sequencing of Wolbachia surface protein (wsp) gene and some polymorphic genomic regions of insertion sites (IS) and variable number tandem repeats (VNTR) loci. The sequence of the Wolbachia surface protein (wsp) gene was 100% identical between hosts. There is no insertion sequence among specimens. The insertion sequence IS-WD1310 was identical between wMel from both hosts and among other strains, as well as the IS-WD516/7. The VNTR-141 period was identical within wMel from both hosts and among other strains, the VNTR-105 as well. Wolbachia Yogya field-caught D. melanogaster and Wolbachia strain wMel present in Ae. aegypti used for bio-control of dengue were genetically identical. These findings provide beneficial understanding to answer the public attention on safety issues, especially on the genetic similarity between Wolbachia strain in the natural and transfected hosts of this novel technology for dengue control. |
| first_indexed | 2024-03-14T00:05:41Z |
| format | Article |
| id | oai:generic.eprints.org:282740 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:05:41Z |
| publishDate | 2022 |
| publisher | Society for Indonesian Biodiversity |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2827402023-11-16T07:09:50Z https://repository.ugm.ac.id/282740/ Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host Rovik, Anwar Daniwijaya, Edwin Widyanto Supriyati, Endah Rahayu,, Ayu Kumalawati, Dian Aruni Saraswati, Utari Handayaningsih, Anastasia Ev Rachman, Mifta Pratiwi Oktriani, Risky Kurniasari, Irianti Candrasari, Damiana Sapta Nurhayati, Indah Sholeh, Rizki Arianto, Budi Tantowijoyo, Warsito Ahmad, Riris Andono Utarini, Adi Arguni, Eggi Medical Virology Aedes aegypti as a novel host. Biodiversitas 23: 2321-2328. Wolbachia naturally presents in a large number of insects and other arthropod species. The Wolbachia strain wMel from Drosophila melanogaster has been stably transinfected into Aedes aegypti where it stops the mosquito host from being infected with medically important arbovirus like dengue. Consequently, Ae. aegypti infected with wMel have been released in Indonesia as a public health intervention against dengue. This study genetically compared wMel from Yogya field-caught D. melanogaster and the wMel in stably transfected Ae. aegypti used for field releases in Yogyakarta, Indonesia. The genetic similarity between wMel Wolbachia was evaluated by sequencing of Wolbachia surface protein (wsp) gene and some polymorphic genomic regions of insertion sites (IS) and variable number tandem repeats (VNTR) loci. The sequence of the Wolbachia surface protein (wsp) gene was 100% identical between hosts. There is no insertion sequence among specimens. The insertion sequence IS-WD1310 was identical between wMel from both hosts and among other strains, as well as the IS-WD516/7. The VNTR-141 period was identical within wMel from both hosts and among other strains, the VNTR-105 as well. Wolbachia Yogya field-caught D. melanogaster and Wolbachia strain wMel present in Ae. aegypti used for bio-control of dengue were genetically identical. These findings provide beneficial understanding to answer the public attention on safety issues, especially on the genetic similarity between Wolbachia strain in the natural and transfected hosts of this novel technology for dengue control. Society for Indonesian Biodiversity 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/282740/1/8.pdf Rovik, Anwar and Daniwijaya, Edwin Widyanto and Supriyati, Endah and Rahayu,, Ayu and Kumalawati, Dian Aruni and Saraswati, Utari and Handayaningsih, Anastasia Ev and Rachman, Mifta Pratiwi and Oktriani, Risky and Kurniasari, Irianti and Candrasari, Damiana Sapta and Nurhayati, Indah and Sholeh, Rizki and Arianto, Budi and Tantowijoyo, Warsito and Ahmad, Riris Andono and Utarini, Adi and Arguni, Eggi (2022) Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host. BIODIVERSITAS, 23 (5). pp. 2321-2328. ISSN 2085-4722 https://smujo.id/biodiv/article/view/10558/5717 10.13057/biodiv/d230510 |
| spellingShingle | Medical Virology Rovik, Anwar Daniwijaya, Edwin Widyanto Supriyati, Endah Rahayu,, Ayu Kumalawati, Dian Aruni Saraswati, Utari Handayaningsih, Anastasia Ev Rachman, Mifta Pratiwi Oktriani, Risky Kurniasari, Irianti Candrasari, Damiana Sapta Nurhayati, Indah Sholeh, Rizki Arianto, Budi Tantowijoyo, Warsito Ahmad, Riris Andono Utarini, Adi Arguni, Eggi Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host |
| title | Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host |
| title_full | Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host |
| title_fullStr | Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host |
| title_full_unstemmed | Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host |
| title_short | Wolbachia genetic similarity in different insect host species: Drosophila melanogaster and Yogyakarta’s (Indonesia) Aedes aegypti as a novel host |
| title_sort | wolbachia genetic similarity in different insect host species drosophila melanogaster and yogyakarta s indonesia aedes aegypti as a novel host |
| topic | Medical Virology |
| url | https://repository.ugm.ac.id/282740/1/8.pdf |
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