Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells
We investigated roles of Hippo signaling pathway components in alveolar type II cells (AECII) after zinc oxide nanoparticle (ZnONP) exposure. ZnONPs physicochemistry was characterized using field emission-scanning electron microscopy (FE-SEM) and energy-dispersive X-ray (EDX) microanalysis. ZnONP de...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2022
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| Online Access: | https://repository.ugm.ac.id/283030/1/211.pdf |
| _version_ | 1826050571444944896 |
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| author | Laiman, Vincent Heriyanto, Didik Setyo Lee, Yueh-Lun Lai, Ching-Huang Pan, Chih-Hong Chen, Wei-Liang Wang, Chung-Ching Chuang, Kai-Jen Chang, Jer-Hwa Chuang, Hsiao-Chi |
| author_facet | Laiman, Vincent Heriyanto, Didik Setyo Lee, Yueh-Lun Lai, Ching-Huang Pan, Chih-Hong Chen, Wei-Liang Wang, Chung-Ching Chuang, Kai-Jen Chang, Jer-Hwa Chuang, Hsiao-Chi |
| author_sort | Laiman, Vincent |
| collection | UGM |
| description | We investigated roles of Hippo signaling pathway components in alveolar type II cells (AECII) after zinc oxide nanoparticle (ZnONP) exposure. ZnONPs physicochemistry was characterized using field emission-scanning electron microscopy (FE-SEM) and energy-dispersive X-ray (EDX) microanalysis. ZnONP deposition in human respiratory tract was estimated using multiple-path particle dosimetry (MPPD) model. MLE-12 AECII were cultured and exposed to 0, 1, and 5 μg/mL of ZnONPs for 24 h. Western blots were used to investigate signaling pathways associated with Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ), cell adherens junctions, differentiation, and senescence. ZnONPs morphology was irregular, with Zn and O identified. Approximately 72% of inhaled ZnONPs were deposited in lungs, with 26% being deposited in alveolar regions. ZnONP exposure increased nuclear YAP expression and decreased cytoplasmic YAP expression by AECII. Adherens junction proteins, E-cadherin, α-catenin, and β-catenin, on AECII decreased after ZnONP exposure. ZnONP exposure of AECII increased alveolar type I (AECI) transition protein, LGALS3, and the AECI protein, T1α, while decreasing AECII SPC expression. ZnONP exposure induced Sirt1 and p53 senescence proteins by AECII. Our findings showed that inhalable ZnONPs can deposit in alveoli, which promotes YAP nuclear localization in AECII, resulting in decrease tight junctions, cell differentiation, and cell senescence. |
| first_indexed | 2024-03-14T00:06:07Z |
| format | Article |
| id | oai:generic.eprints.org:283030 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:06:07Z |
| publishDate | 2022 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2830302023-11-17T08:24:10Z https://repository.ugm.ac.id/283030/ Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells Laiman, Vincent Heriyanto, Didik Setyo Lee, Yueh-Lun Lai, Ching-Huang Pan, Chih-Hong Chen, Wei-Liang Wang, Chung-Ching Chuang, Kai-Jen Chang, Jer-Hwa Chuang, Hsiao-Chi Cell Physiology We investigated roles of Hippo signaling pathway components in alveolar type II cells (AECII) after zinc oxide nanoparticle (ZnONP) exposure. ZnONPs physicochemistry was characterized using field emission-scanning electron microscopy (FE-SEM) and energy-dispersive X-ray (EDX) microanalysis. ZnONP deposition in human respiratory tract was estimated using multiple-path particle dosimetry (MPPD) model. MLE-12 AECII were cultured and exposed to 0, 1, and 5 μg/mL of ZnONPs for 24 h. Western blots were used to investigate signaling pathways associated with Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ), cell adherens junctions, differentiation, and senescence. ZnONPs morphology was irregular, with Zn and O identified. Approximately 72% of inhaled ZnONPs were deposited in lungs, with 26% being deposited in alveolar regions. ZnONP exposure increased nuclear YAP expression and decreased cytoplasmic YAP expression by AECII. Adherens junction proteins, E-cadherin, α-catenin, and β-catenin, on AECII decreased after ZnONP exposure. ZnONP exposure of AECII increased alveolar type I (AECI) transition protein, LGALS3, and the AECI protein, T1α, while decreasing AECII SPC expression. ZnONP exposure induced Sirt1 and p53 senescence proteins by AECII. Our findings showed that inhalable ZnONPs can deposit in alveoli, which promotes YAP nuclear localization in AECII, resulting in decrease tight junctions, cell differentiation, and cell senescence. Multidisciplinary Digital Publishing Institute 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283030/1/211.pdf Laiman, Vincent and Heriyanto, Didik Setyo and Lee, Yueh-Lun and Lai, Ching-Huang and Pan, Chih-Hong and Chen, Wei-Liang and Wang, Chung-Ching and Chuang, Kai-Jen and Chang, Jer-Hwa and Chuang, Hsiao-Chi (2022) Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells. Atmosphere, 13 (2). p. 334. ISSN 2073-4433 https://www.mdpi.com/2073-4433/13/2/334 10.3390/atmos13020334 |
| spellingShingle | Cell Physiology Laiman, Vincent Heriyanto, Didik Setyo Lee, Yueh-Lun Lai, Ching-Huang Pan, Chih-Hong Chen, Wei-Liang Wang, Chung-Ching Chuang, Kai-Jen Chang, Jer-Hwa Chuang, Hsiao-Chi Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_full | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_fullStr | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_full_unstemmed | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_short | Zinc Oxide Nanoparticles Promote YAP/TAZ Nuclear Localization in Alveolar Epithelial Type II Cells |
| title_sort | zinc oxide nanoparticles promote yap taz nuclear localization in alveolar epithelial type ii cells |
| topic | Cell Physiology |
| url | https://repository.ugm.ac.id/283030/1/211.pdf |
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