| Summary: | Embryo transfer is a reproductive technology that shortens the generation interval between selection steps by producing a high percentage of donor progeny. The success of post transfer pregnancy determines the success of the embryo transfer, but the implantation rate in Indonesia is not yet optimal; measures must be taken to improve the condition of the acceptor. Therefore, this study was carried out to determine the diameter of the corpus luteum and endometrial thickness from local breed, age, parity status, body condition score (BCS) with its effect on progesterone levels and to determine the standard size of the corpus luteum and endometrium. Furthermore, the selected cows were in normal reproductive status. Therefore, they were rectally cycled, and ultrasound (USG) was used to measure the corpus luteum diameter and the wall thickness of the endometrium wall. In addition, blood samples were taken from 7-day-old cows in the oestrus cycle to determine the level of the hormone progesterone using the ELISA test. Of the 15 beef cattle, 9 are PO cattle, and 6 are crossbred cows. The results showed that endometrial thickness was a factor that had a positive correlation with progesterone levels. The highest level of progesterone in crossbreed cows of 12.9 ng/ml resulted in an endometrial thickness of 9 mm, while the highest level of progesterone in PO cattle with 13.8 ng/ml resulted in an endometrial thickness of 5 mm with the optimal standard of the best progesterone levels for PO beef cattle and crossbreed recipients are cows with endometrial thickness > 6 mm, 5 years old, and parity 2x calf. In conclusion, the reproductive status of the ovaries, corpus luteum, progesterone levels, and endometrial thickness are factors that must be considered in increasing the success rate of embryo transfer. © Published under licence by IOP Publishing Ltd.
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