The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro
Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime coun...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Society for Indonesian Biodiversity
2022
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| Online Access: | https://repository.ugm.ac.id/283135/1/11750-Article%20Text-1065576-1-10-20221102.pdf |
| _version_ | 1826050591152930816 |
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| author | Afililla, Zhaza Suwanti, Lucia Tri Mufasirin, Mufasirin Koerniawan, Heni Pny Suyono, Eko Agus Budiman, Arief Siregar, Ulfah Juniarti |
| author_facet | Afililla, Zhaza Suwanti, Lucia Tri Mufasirin, Mufasirin Koerniawan, Heni Pny Suyono, Eko Agus Budiman, Arief Siregar, Ulfah Juniarti |
| author_sort | Afililla, Zhaza |
| collection | UGM |
| description | Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime country that has various organisms, including microalgae. Some microalgae have many bioactivities, such as immunomodulatory activity. This study aimed to determine Glagah consortium microalgae polysaccharide (GCMP) and polysaccharide of Spirulina platensis (PSP) as an immunomodulator based on its effect on splenocyte proliferation and cytokine production in vitro. Crude polysaccharides were extracted by lye extraction method. Splenocytes were obtained from spleen of healthy mice and cultured in RPMI 1640 medium with and without Concanavalin A (Con A). Cells (3×105 per well) were treated with polysaccharide with dose gradually 0, 1.25, 2.5, 10 and 20 μg/mL. Splenocytes proliferation were observed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The supernatant was taken for the detection of cytokines using enzyme-linked immunoassay (ELISA). The results showed that GCMP, both with and without Con A at a concentration of less than 10 mg/mL decreased cell viability, on the other hand, a concentration of 10-20 mg/mL had a proliferative effect. Splenocytes were shown a proliferative effect at all concentrations of PSP, both with and without Con A. The concentration of polysaccharides, both GCMP and PSP, did not affect cytokine levels. In conclusion, based on the proliferation of splenocytes, GCMP has an immunomodulatory effect depending on its concentration. |
| first_indexed | 2024-03-14T00:06:39Z |
| format | Article |
| id | oai:generic.eprints.org:283135 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:06:39Z |
| publishDate | 2022 |
| publisher | Society for Indonesian Biodiversity |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2831352023-11-22T08:32:33Z https://repository.ugm.ac.id/283135/ The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro Afililla, Zhaza Suwanti, Lucia Tri Mufasirin, Mufasirin Koerniawan, Heni Pny Suyono, Eko Agus Budiman, Arief Siregar, Ulfah Juniarti Veterinary Medicine Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime country that has various organisms, including microalgae. Some microalgae have many bioactivities, such as immunomodulatory activity. This study aimed to determine Glagah consortium microalgae polysaccharide (GCMP) and polysaccharide of Spirulina platensis (PSP) as an immunomodulator based on its effect on splenocyte proliferation and cytokine production in vitro. Crude polysaccharides were extracted by lye extraction method. Splenocytes were obtained from spleen of healthy mice and cultured in RPMI 1640 medium with and without Concanavalin A (Con A). Cells (3×105 per well) were treated with polysaccharide with dose gradually 0, 1.25, 2.5, 10 and 20 μg/mL. Splenocytes proliferation were observed by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The supernatant was taken for the detection of cytokines using enzyme-linked immunoassay (ELISA). The results showed that GCMP, both with and without Con A at a concentration of less than 10 mg/mL decreased cell viability, on the other hand, a concentration of 10-20 mg/mL had a proliferative effect. Splenocytes were shown a proliferative effect at all concentrations of PSP, both with and without Con A. The concentration of polysaccharides, both GCMP and PSP, did not affect cytokine levels. In conclusion, based on the proliferation of splenocytes, GCMP has an immunomodulatory effect depending on its concentration. Society for Indonesian Biodiversity 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283135/1/11750-Article%20Text-1065576-1-10-20221102.pdf Afililla, Zhaza and Suwanti, Lucia Tri and Mufasirin, Mufasirin and Koerniawan, Heni Pny and Suyono, Eko Agus and Budiman, Arief and Siregar, Ulfah Juniarti (2022) The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas, 23 (10). 5205 – 5209. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85140412406&doi=10.13057%2fbiodiv%2fd231028&partnerID=40&md5=1b0f0b606a1a6b81f6f2d17068448f68 10.13057/biodiv/d231028 |
| spellingShingle | Veterinary Medicine Afililla, Zhaza Suwanti, Lucia Tri Mufasirin, Mufasirin Koerniawan, Heni Pny Suyono, Eko Agus Budiman, Arief Siregar, Ulfah Juniarti The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| title | The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| title_full | The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| title_fullStr | The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| title_full_unstemmed | The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| title_short | The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| title_sort | immunomodulatory effect of glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro |
| topic | Veterinary Medicine |
| url | https://repository.ugm.ac.id/283135/1/11750-Article%20Text-1065576-1-10-20221102.pdf |
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