Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur
Septicemia epizootica (SE) is a common fatal systemic disease in cattle and buffalo due to Pasteurella multocida serotype B:2 in South and Southeast Asia countries, including Indonesia. This infectious agent is generally considered an opportunistic pathogen and located in the nasopharynx of the anim...
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| Format: | Conference or Workshop Item |
| Language: | English English |
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IOP Publishing Ltd
2022
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| Online Access: | https://repository.ugm.ac.id/283142/1/scopus%2823%29.bib https://repository.ugm.ac.id/283142/2/Prihandani_2022_IOP_Conf._Ser.%20_Earth_Environ._Sci._976_012046.pdf |
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| author | Prihandani, S.S. Noor, S.M. Wibawan, I.W.T. Safika, Safika Purba, H.H.S. Sumirah, Sumirah Kusumawati, A. |
| author_facet | Prihandani, S.S. Noor, S.M. Wibawan, I.W.T. Safika, Safika Purba, H.H.S. Sumirah, Sumirah Kusumawati, A. |
| author_sort | Prihandani, S.S. |
| collection | UGM |
| description | Septicemia epizootica (SE) is a common fatal systemic disease in cattle and buffalo due to Pasteurella multocida serotype B:2 in South and Southeast Asia countries, including Indonesia. This infectious agent is generally considered an opportunistic pathogen and located in the nasopharynx of the animal. To support the disease's diagnosis, an acceptable identification procedure must be established. This study was to confirm bovine P. multocida isolate that has been identified through biochemical approaches at the first step, with the Polymerase Chain Reaction method. The isolate was obtained from the fatal outbreak of cattle in Kupang in 2016 and subjected to be identified using biochemical characterization, but it was time consumed. The API 20NE was applied to identify the isolate and help to save time. The PCR result showed positive for 16 sRNA and kmt genes, both were classified as specific genes, and the capsular serotype was detected in less than 24 hours. It indicates that PCR confirms biochemical technique, and it is an appropriate and faster method in detecting pathogen agents than biochemical one. © Published under licence by IOP Publishing Ltd. |
| first_indexed | 2024-03-14T00:06:41Z |
| format | Conference or Workshop Item |
| id | oai:generic.eprints.org:283142 |
| institution | Universiti Gadjah Mada |
| language | English English |
| last_indexed | 2024-03-14T00:06:41Z |
| publishDate | 2022 |
| publisher | IOP Publishing Ltd |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2831422023-11-24T02:27:38Z https://repository.ugm.ac.id/283142/ Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur Prihandani, S.S. Noor, S.M. Wibawan, I.W.T. Safika, Safika Purba, H.H.S. Sumirah, Sumirah Kusumawati, A. Veterinary Medicine Septicemia epizootica (SE) is a common fatal systemic disease in cattle and buffalo due to Pasteurella multocida serotype B:2 in South and Southeast Asia countries, including Indonesia. This infectious agent is generally considered an opportunistic pathogen and located in the nasopharynx of the animal. To support the disease's diagnosis, an acceptable identification procedure must be established. This study was to confirm bovine P. multocida isolate that has been identified through biochemical approaches at the first step, with the Polymerase Chain Reaction method. The isolate was obtained from the fatal outbreak of cattle in Kupang in 2016 and subjected to be identified using biochemical characterization, but it was time consumed. The API 20NE was applied to identify the isolate and help to save time. The PCR result showed positive for 16 sRNA and kmt genes, both were classified as specific genes, and the capsular serotype was detected in less than 24 hours. It indicates that PCR confirms biochemical technique, and it is an appropriate and faster method in detecting pathogen agents than biochemical one. © Published under licence by IOP Publishing Ltd. IOP Publishing Ltd 2022 Conference or Workshop Item PeerReviewed text/html en https://repository.ugm.ac.id/283142/1/scopus%2823%29.bib application/pdf en https://repository.ugm.ac.id/283142/2/Prihandani_2022_IOP_Conf._Ser.%20_Earth_Environ._Sci._976_012046.pdf Prihandani, S.S. and Noor, S.M. and Wibawan, I.W.T. and Safika, Safika and Purba, H.H.S. and Sumirah, Sumirah and Kusumawati, A. (2022) Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur. In: 2nd International Conference on Tropical Wetland Biodiversity and Conservation. https://iopscience.iop.org/article/10.1088/1755-1315/976/1/012046 |
| spellingShingle | Veterinary Medicine Prihandani, S.S. Noor, S.M. Wibawan, I.W.T. Safika, Safika Purba, H.H.S. Sumirah, Sumirah Kusumawati, A. Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur |
| title | Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur |
| title_full | Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur |
| title_fullStr | Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur |
| title_full_unstemmed | Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur |
| title_short | Polymerase chain reaction to confirm biochemically characterization method of Pasteurella multocida isolate from fatal cases of Septicaemia epizootica in Nusa Tenggara Timur |
| title_sort | polymerase chain reaction to confirm biochemically characterization method of pasteurella multocida isolate from fatal cases of septicaemia epizootica in nusa tenggara timur |
| topic | Veterinary Medicine |
| url | https://repository.ugm.ac.id/283142/1/scopus%2823%29.bib https://repository.ugm.ac.id/283142/2/Prihandani_2022_IOP_Conf._Ser.%20_Earth_Environ._Sci._976_012046.pdf |
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