Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand
The study of bacterial zoonoses has been under-pursued despite the fact that bacteria cause the majority of zoonotic diseases, of which 70 have a wildlife origin. More Bartonella species are being identified as the cause of human diseases, and several of them have been linked to domestic and wild an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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John Wiley and Sons Inc
2022
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| Online Access: | https://repository.ugm.ac.id/283152/1/scopus%2830%29.bib |
| _version_ | 1797037642907385856 |
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| author | Poofery, Juthathip Narapakdeesakul, Duriyang Riana, Elizabeth Arnuphapprasert, Apinya Nugraheni, Yudhi Ratna Ngamprasertwong, Thongchai Wangthongchaicharoen, Monsicha Soisook, Pipat Bhodhibundit, Phanaschakorn Kaewthamasorn, Morakot |
| author_facet | Poofery, Juthathip Narapakdeesakul, Duriyang Riana, Elizabeth Arnuphapprasert, Apinya Nugraheni, Yudhi Ratna Ngamprasertwong, Thongchai Wangthongchaicharoen, Monsicha Soisook, Pipat Bhodhibundit, Phanaschakorn Kaewthamasorn, Morakot |
| author_sort | Poofery, Juthathip |
| collection | UGM |
| description | The study of bacterial zoonoses has been under-pursued despite the fact that bacteria cause the majority of zoonotic diseases, of which 70 have a wildlife origin. More Bartonella species are being identified as the cause of human diseases, and several of them have been linked to domestic and wild animals. Bats are outstanding reservoirs for Bartonella species because of their wide distribution, mobility, roosting behaviour, and long life span. Here, we carried out a PCR-based survey on bats that were collected from 19 sampling sites in eight provinces of Thailand from February 2018 to April 2021. Bartonella infection was investigated in a total of 459 bats that belong to 24 different bat species (21 species of which had never been previously studied in Thailand). PCR diagnostics revealed that 115 out of 459 (25.5) blood samples tested positive for Bartonella. The nucleotide identities of the Bartonella 16S rRNA sequences in this study were between 95.78-99.66 identical to those of known zoonotic species (Bartonella ancashensis, Bartonella henselae, Bartonella bacilliformis and Bartonella australis) as well as to an unidentified Bartonella spp. In addition, the citrate synthase (gltA) and RNA polymerase-beta subunit (rpoB) genes of Bartonella were sequenced and analyzed in positive samples. The gltA and rpoB gene sequences from Hipposideros gentilis and Rhinolophus coelophyllus bat samples showed low nucleotide identity (<95) compared to those of the currently deposited sequences in the GenBank database, indicating the possibility of new Bartonella species. The phylogenetic inference and genetic diversity were generated and indicated a close relationship with other Bartonella species previously discovered in Asian bats. Overall, the current study demonstrates the primary evidence pointing to a potential novel Bartonella species in bats. This discovery also contributes to our current understanding of the geographical distribution, genetic diversity, and host ranges of bat-related Bartonella. © 2021 Wiley-VCH GmbH. |
| first_indexed | 2024-03-14T00:06:43Z |
| format | Article |
| id | oai:generic.eprints.org:283152 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:06:43Z |
| publishDate | 2022 |
| publisher | John Wiley and Sons Inc |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2831522023-11-24T02:45:04Z https://repository.ugm.ac.id/283152/ Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand Poofery, Juthathip Narapakdeesakul, Duriyang Riana, Elizabeth Arnuphapprasert, Apinya Nugraheni, Yudhi Ratna Ngamprasertwong, Thongchai Wangthongchaicharoen, Monsicha Soisook, Pipat Bhodhibundit, Phanaschakorn Kaewthamasorn, Morakot Veterinary Medicine The study of bacterial zoonoses has been under-pursued despite the fact that bacteria cause the majority of zoonotic diseases, of which 70 have a wildlife origin. More Bartonella species are being identified as the cause of human diseases, and several of them have been linked to domestic and wild animals. Bats are outstanding reservoirs for Bartonella species because of their wide distribution, mobility, roosting behaviour, and long life span. Here, we carried out a PCR-based survey on bats that were collected from 19 sampling sites in eight provinces of Thailand from February 2018 to April 2021. Bartonella infection was investigated in a total of 459 bats that belong to 24 different bat species (21 species of which had never been previously studied in Thailand). PCR diagnostics revealed that 115 out of 459 (25.5) blood samples tested positive for Bartonella. The nucleotide identities of the Bartonella 16S rRNA sequences in this study were between 95.78-99.66 identical to those of known zoonotic species (Bartonella ancashensis, Bartonella henselae, Bartonella bacilliformis and Bartonella australis) as well as to an unidentified Bartonella spp. In addition, the citrate synthase (gltA) and RNA polymerase-beta subunit (rpoB) genes of Bartonella were sequenced and analyzed in positive samples. The gltA and rpoB gene sequences from Hipposideros gentilis and Rhinolophus coelophyllus bat samples showed low nucleotide identity (<95) compared to those of the currently deposited sequences in the GenBank database, indicating the possibility of new Bartonella species. The phylogenetic inference and genetic diversity were generated and indicated a close relationship with other Bartonella species previously discovered in Asian bats. Overall, the current study demonstrates the primary evidence pointing to a potential novel Bartonella species in bats. This discovery also contributes to our current understanding of the geographical distribution, genetic diversity, and host ranges of bat-related Bartonella. © 2021 Wiley-VCH GmbH. John Wiley and Sons Inc 2022 Article PeerReviewed text/html en https://repository.ugm.ac.id/283152/1/scopus%2830%29.bib Poofery, Juthathip and Narapakdeesakul, Duriyang and Riana, Elizabeth and Arnuphapprasert, Apinya and Nugraheni, Yudhi Ratna and Ngamprasertwong, Thongchai and Wangthongchaicharoen, Monsicha and Soisook, Pipat and Bhodhibundit, Phanaschakorn and Kaewthamasorn, Morakot (2022) Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand. Transboundary and Emerging Diseases, 69 (4). e717 – e733. https://onlinelibrary.wiley.com/doi/10.1111/tbed.14389 10.1111/tbed.14389 |
| spellingShingle | Veterinary Medicine Poofery, Juthathip Narapakdeesakul, Duriyang Riana, Elizabeth Arnuphapprasert, Apinya Nugraheni, Yudhi Ratna Ngamprasertwong, Thongchai Wangthongchaicharoen, Monsicha Soisook, Pipat Bhodhibundit, Phanaschakorn Kaewthamasorn, Morakot Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand |
| title | Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand |
| title_full | Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand |
| title_fullStr | Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand |
| title_full_unstemmed | Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand |
| title_short | Molecular identification and genetic diversity of Bartonella spp. in 24 bat species from Thailand |
| title_sort | molecular identification and genetic diversity of bartonella spp in 24 bat species from thailand |
| topic | Veterinary Medicine |
| url | https://repository.ugm.ac.id/283152/1/scopus%2830%29.bib |
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