Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels
Obesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of Caul...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022
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| Online Access: | https://repository.ugm.ac.id/283199/1/26.pdf |
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| author | Permatasari, Happy Kurnia Nurkolis, Fahrul Hardinsyah, Hardinsyah Taslim, Nurpudji Astuti Sabrina, Nindy Ibrahim, Faisal Maulana Visnu, Jodi Kumalawati, Dian Aruni Febriana, Sri Awalia Sudargo, Toto Tanner, Melvin Junior Kurniatanty, Isma Yusuf, Vincentius Mario Rompies, Ronald Bahar, Muhammad Rahimi Holipah, Holipah Mayulu, Nelly |
| author_facet | Permatasari, Happy Kurnia Nurkolis, Fahrul Hardinsyah, Hardinsyah Taslim, Nurpudji Astuti Sabrina, Nindy Ibrahim, Faisal Maulana Visnu, Jodi Kumalawati, Dian Aruni Febriana, Sri Awalia Sudargo, Toto Tanner, Melvin Junior Kurniatanty, Isma Yusuf, Vincentius Mario Rompies, Ronald Bahar, Muhammad Rahimi Holipah, Holipah Mayulu, Nelly |
| author_sort | Permatasari, Happy Kurnia |
| collection | UGM |
| description | Obesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of Caulerpa racemosa done in a previous preclinical trial. Six major compounds from sea grape (C. racemosa) extract were evaluated using an in silico approach against human pancreatic lipase, a-glucosidase, and a-amylase to predict prospective anti-obesity candidates. The lipase inhibitory activity of the extract reached 90.30 ± 0.40, 1.75 lower than orlistat. The a-amylase inhibitory assay of the extract was 84.07 ± 5.28, while the inhibitory activity against a-glucosidase was 81.67 ± 1.54; both were lower than acarbose. We observe the effect of C. racemosa extract as anti-obesity with anti-aging by evaluating the obesity parameters in the human body for a 4-week period. There was a significant decrease in blood glucose, total cholesterol, low-density lipoprotein (LDL), triglycerides (TG), waist circumference, waist-hip ratio, and body weight (p < 0.05); PGC-1α and high-density lipoprotein (HDL) increased significantly (p = 0.000), in Group B when compared with Group A. Our study revealed that sea grape extract is a potent anti-obesity with an anti-aging reagent that does not produce any significant adverse effects. Copyright © 2022 Permatasari, Nurkolis, Hardinsyah, Taslim, Sabrina, Ibrahim, Visnu, Kumalawati, Febriana, Sudargo, Tanner, Kurniatanty, Yusuf, Rompies, Bahar, Holipah and Mayulu. |
| first_indexed | 2024-03-14T00:07:00Z |
| format | Article |
| id | oai:generic.eprints.org:283199 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:07:00Z |
| publishDate | 2022 |
| publisher | Frontiers Media S.A. |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2831992023-11-20T03:19:24Z https://repository.ugm.ac.id/283199/ Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels Permatasari, Happy Kurnia Nurkolis, Fahrul Hardinsyah, Hardinsyah Taslim, Nurpudji Astuti Sabrina, Nindy Ibrahim, Faisal Maulana Visnu, Jodi Kumalawati, Dian Aruni Febriana, Sri Awalia Sudargo, Toto Tanner, Melvin Junior Kurniatanty, Isma Yusuf, Vincentius Mario Rompies, Ronald Bahar, Muhammad Rahimi Holipah, Holipah Mayulu, Nelly Metabolic Medicine Obesity is associated with an accelerated aging process, which prevents healthy aging. Both obesity and aging were manifested in the peroxisome proliferator-activated receptor-γ coactivator α (PGC-1α) level. These studies fulfill the scientific gap in assembled pharmacological activity assay of Caulerpa racemosa done in a previous preclinical trial. Six major compounds from sea grape (C. racemosa) extract were evaluated using an in silico approach against human pancreatic lipase, a-glucosidase, and a-amylase to predict prospective anti-obesity candidates. The lipase inhibitory activity of the extract reached 90.30 ± 0.40, 1.75 lower than orlistat. The a-amylase inhibitory assay of the extract was 84.07 ± 5.28, while the inhibitory activity against a-glucosidase was 81.67 ± 1.54; both were lower than acarbose. We observe the effect of C. racemosa extract as anti-obesity with anti-aging by evaluating the obesity parameters in the human body for a 4-week period. There was a significant decrease in blood glucose, total cholesterol, low-density lipoprotein (LDL), triglycerides (TG), waist circumference, waist-hip ratio, and body weight (p < 0.05); PGC-1α and high-density lipoprotein (HDL) increased significantly (p = 0.000), in Group B when compared with Group A. Our study revealed that sea grape extract is a potent anti-obesity with an anti-aging reagent that does not produce any significant adverse effects. Copyright © 2022 Permatasari, Nurkolis, Hardinsyah, Taslim, Sabrina, Ibrahim, Visnu, Kumalawati, Febriana, Sudargo, Tanner, Kurniatanty, Yusuf, Rompies, Bahar, Holipah and Mayulu. Frontiers Media S.A. 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283199/1/26.pdf Permatasari, Happy Kurnia and Nurkolis, Fahrul and Hardinsyah, Hardinsyah and Taslim, Nurpudji Astuti and Sabrina, Nindy and Ibrahim, Faisal Maulana and Visnu, Jodi and Kumalawati, Dian Aruni and Febriana, Sri Awalia and Sudargo, Toto and Tanner, Melvin Junior and Kurniatanty, Isma and Yusuf, Vincentius Mario and Rompies, Ronald and Bahar, Muhammad Rahimi and Holipah, Holipah and Mayulu, Nelly (2022) Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels. Frontiers in Nutrition, 9. ISSN 2296-861X https://www.scopus.com/inward/record.uri?eid=2-s2.0-85135036324&doi=10.3389%2ffnut.2022.939073&partnerID=40&md5=304afd2ddf5c04fab82ed36217a81d6b 10.3389/fnut.2022.939073 |
| spellingShingle | Metabolic Medicine Permatasari, Happy Kurnia Nurkolis, Fahrul Hardinsyah, Hardinsyah Taslim, Nurpudji Astuti Sabrina, Nindy Ibrahim, Faisal Maulana Visnu, Jodi Kumalawati, Dian Aruni Febriana, Sri Awalia Sudargo, Toto Tanner, Melvin Junior Kurniatanty, Isma Yusuf, Vincentius Mario Rompies, Ronald Bahar, Muhammad Rahimi Holipah, Holipah Mayulu, Nelly Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_full | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_fullStr | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_full_unstemmed | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_short | Metabolomic Assay, Computational Screening, and Pharmacological Evaluation of Caulerpa racemosa as an Anti-obesity With Anti-aging by Altering Lipid Profile and Peroxisome Proliferator-Activated Receptor-γ Coactivator 1-α Levels |
| title_sort | metabolomic assay computational screening and pharmacological evaluation of caulerpa racemosa as an anti obesity with anti aging by altering lipid profile and peroxisome proliferator activated receptor γ coactivator 1 α levels |
| topic | Metabolic Medicine |
| url | https://repository.ugm.ac.id/283199/1/26.pdf |
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