The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast
Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5‐fluorouracil (5‐FU) has already been use...
| المؤلفون الرئيسيون: | , , |
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| التنسيق: | مقال |
| اللغة: | English |
| منشور في: |
Universitas Gadjah Mada, Research Center for Biotechnology
2022
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://repository.ugm.ac.id/283312/1/document.pdf |
| _version_ | 1826050624229212160 |
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| author | Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo |
| author_facet | Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo |
| author_sort | Amelia, Jesslyn |
| collection | UGM |
| description | Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5‐fluorouracil (5‐FU) has already been used in keloid management. This study aimed to determine the efficacy of the combination of captopril and 5‐FU in keloid fibroblast cultures. Keloid tissues were cultured up to passages 4–7. The study consisted of a control group, captopril in various concentrations (10‐2, 10‐3, 10‐4, and 10‐5 mol/L), 5‐FU 1 mg/mL and a combination of captopril at various concentrations with 5‐FU 1 mg/mL. After 144 hours of treatment, fibroblast proliferation and collagen deposition were measured. The study showed a significant decrease in the mean index of fibroblast proliferation and collagen deposition in the group receiving captopril in various concentrations (10‐2, 10‐3, 10‐4, and 10‐5 mol/L) and the 5‐FU group against the control group (p<0.05). In the combined‐dose group, captopril at a concentration of 10‐2 mol/L and 5‐FU showed a significant reduction in fibroblast proliferation and collagen deposition compared to the 5‐FU group and the captopril at the same dose (p<0.05). In conclusion, the combination of captopril 10‐2 mol/L and 5‐FU 1 mg/mL is better at reducing fibroblast proliferation and collagen deposition in keloid fibroblast cultures than captopril or 5‐FU as a single therapeutic agent. |
| first_indexed | 2024-03-14T00:07:31Z |
| format | Article |
| id | oai:generic.eprints.org:283312 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:07:31Z |
| publishDate | 2022 |
| publisher | Universitas Gadjah Mada, Research Center for Biotechnology |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2833122023-11-20T06:24:36Z https://repository.ugm.ac.id/283312/ The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo Dermatology Keloid is a benign fibroproliferative tissue growth that exceeds the initial wound margins. Captopril has been tested in vitro to reduce fibroblast proliferation and collagen deposition; thus, it has potential for use in the treatment of keloids. Meanwhile, 5‐fluorouracil (5‐FU) has already been used in keloid management. This study aimed to determine the efficacy of the combination of captopril and 5‐FU in keloid fibroblast cultures. Keloid tissues were cultured up to passages 4–7. The study consisted of a control group, captopril in various concentrations (10‐2, 10‐3, 10‐4, and 10‐5 mol/L), 5‐FU 1 mg/mL and a combination of captopril at various concentrations with 5‐FU 1 mg/mL. After 144 hours of treatment, fibroblast proliferation and collagen deposition were measured. The study showed a significant decrease in the mean index of fibroblast proliferation and collagen deposition in the group receiving captopril in various concentrations (10‐2, 10‐3, 10‐4, and 10‐5 mol/L) and the 5‐FU group against the control group (p<0.05). In the combined‐dose group, captopril at a concentration of 10‐2 mol/L and 5‐FU showed a significant reduction in fibroblast proliferation and collagen deposition compared to the 5‐FU group and the captopril at the same dose (p<0.05). In conclusion, the combination of captopril 10‐2 mol/L and 5‐FU 1 mg/mL is better at reducing fibroblast proliferation and collagen deposition in keloid fibroblast cultures than captopril or 5‐FU as a single therapeutic agent. Universitas Gadjah Mada, Research Center for Biotechnology 2022-09 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283312/1/document.pdf Amelia, Jesslyn and Siswati, Agnes Sri and Wirohadidjojo, Yohanes Widodo (2022) The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast. Indonesian Journal of Biotechnology, 27 (3). pp. 126-132. ISSN 2089-2241 https://journal.ugm.ac.id/ijbiotech/article/view/69505 10.22146/ijbiotech.69505 |
| spellingShingle | Dermatology Amelia, Jesslyn Siswati, Agnes Sri Wirohadidjojo, Yohanes Widodo The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| title | The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| title_full | The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| title_fullStr | The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| title_full_unstemmed | The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| title_short | The efficacy of captopril and 5-fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| title_sort | efficacy of captopril and 5 fluorouracil combination in the proliferation and collagen deposition of keloid fibroblast |
| topic | Dermatology |
| url | https://repository.ugm.ac.id/283312/1/document.pdf |
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