Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa
Background: Autologous non-cultured cell (ANCC) spray has been used to treat burns, chronic wounds, and vitiligo, but its use in junctional epidermolysis bullosa (JEB) has not been published previously. Chronic wounds in JEB are caused by mutations of laminin 332 (L322), whose function is to attach...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022
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| Online Access: | https://repository.ugm.ac.id/283328/1/CCID-377753-modified-non-cultured-cell-spray-induced-epithelization-in-l.pdf |
| _version_ | 1826050627380183040 |
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| author | Widhiati, Suci Dewi, ShintaTrilaksmi Yefta, Yefta Danarti, Retno Soebono, Hardyanto Irmawati, Yulia Eka Puspitasari, Monika Trisnowati, Niken Wibawa, Tri Purnomosari, Dewajani Wirohadidjojo, Yohanes Widodo |
| author_facet | Widhiati, Suci Dewi, ShintaTrilaksmi Yefta, Yefta Danarti, Retno Soebono, Hardyanto Irmawati, Yulia Eka Puspitasari, Monika Trisnowati, Niken Wibawa, Tri Purnomosari, Dewajani Wirohadidjojo, Yohanes Widodo |
| author_sort | Widhiati, Suci |
| collection | UGM |
| description | Background: Autologous non-cultured cell (ANCC) spray has been used to treat burns, chronic wounds, and vitiligo, but its use in junctional epidermolysis bullosa (JEB) has not been published previously. Chronic wounds in JEB are caused by mutations of laminin 332 (L322), whose function is to attach and act as a glue in the basal membrane. It is proposed that ANCC applications can provide keratinocytes and fibroblasts required to improve epithelization and spontaneously correct revertant keratinocytes in the wound area.
Purpose: To develop a modified procedure of ANCC spray and improve epithelization using silver sulfadiazine covered with plastic wrap to treat chronic wounds of JEB.
Patients and Methods: Shave excision of the donor site was performed on a 19-year-old girl with JEB. The ANCC spray was prepared and applied to the chronic wound, which was then covered with silver sulfadiazine occluded with plastic wrap.
Results: Following the ANCC spray application, epithelization was successfully initiated. Unfortunately, the wounds recurred after four months of follow-up.
Conclusion: The modified application method of ANCC spray provides a good alternative to treat chronic wounds in JEB. |
| first_indexed | 2024-03-14T00:07:34Z |
| format | Article |
| id | oai:generic.eprints.org:283328 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:07:34Z |
| publishDate | 2022 |
| publisher | Dove Medical Press |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2833282023-11-20T06:00:30Z https://repository.ugm.ac.id/283328/ Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa Widhiati, Suci Dewi, ShintaTrilaksmi Yefta, Yefta Danarti, Retno Soebono, Hardyanto Irmawati, Yulia Eka Puspitasari, Monika Trisnowati, Niken Wibawa, Tri Purnomosari, Dewajani Wirohadidjojo, Yohanes Widodo Dermatology Background: Autologous non-cultured cell (ANCC) spray has been used to treat burns, chronic wounds, and vitiligo, but its use in junctional epidermolysis bullosa (JEB) has not been published previously. Chronic wounds in JEB are caused by mutations of laminin 332 (L322), whose function is to attach and act as a glue in the basal membrane. It is proposed that ANCC applications can provide keratinocytes and fibroblasts required to improve epithelization and spontaneously correct revertant keratinocytes in the wound area. Purpose: To develop a modified procedure of ANCC spray and improve epithelization using silver sulfadiazine covered with plastic wrap to treat chronic wounds of JEB. Patients and Methods: Shave excision of the donor site was performed on a 19-year-old girl with JEB. The ANCC spray was prepared and applied to the chronic wound, which was then covered with silver sulfadiazine occluded with plastic wrap. Results: Following the ANCC spray application, epithelization was successfully initiated. Unfortunately, the wounds recurred after four months of follow-up. Conclusion: The modified application method of ANCC spray provides a good alternative to treat chronic wounds in JEB. Dove Medical Press 2022-10-14 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283328/1/CCID-377753-modified-non-cultured-cell-spray-induced-epithelization-in-l.pdf Widhiati, Suci and Dewi, ShintaTrilaksmi and Yefta, Yefta and Danarti, Retno and Soebono, Hardyanto and Irmawati, Yulia Eka and Puspitasari, Monika and Trisnowati, Niken and Wibawa, Tri and Purnomosari, Dewajani and Wirohadidjojo, Yohanes Widodo (2022) Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa. Clinical, Cosmetic and Investigational Dermatology, 15. pp. 2197-2202. ISSN 11787015 https://www.dovepress.com/modified-non-cultured-cell-spray-induced-epithelization-in-lamb3-mutat-peer-reviewed-fulltext-article-CCID 10.2147/CCID.S377753 |
| spellingShingle | Dermatology Widhiati, Suci Dewi, ShintaTrilaksmi Yefta, Yefta Danarti, Retno Soebono, Hardyanto Irmawati, Yulia Eka Puspitasari, Monika Trisnowati, Niken Wibawa, Tri Purnomosari, Dewajani Wirohadidjojo, Yohanes Widodo Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa |
| title | Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa |
| title_full | Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa |
| title_fullStr | Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa |
| title_full_unstemmed | Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa |
| title_short | Modified Non-Cultured Cell Spray Induced Epithelization in LAMB3 Mutation Epidermolysis Bullosa |
| title_sort | modified non cultured cell spray induced epithelization in lamb3 mutation epidermolysis bullosa |
| topic | Dermatology |
| url | https://repository.ugm.ac.id/283328/1/CCID-377753-modified-non-cultured-cell-spray-induced-epithelization-in-l.pdf |
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