Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation
Clove oil is one of the natural antibacterial ingredients that is easily obtained because of its abundant amounts in nature. Various researches have been conducted, but the antibiofilm activity against Candida tropicalis has never been done. This study evaluates the effectiveness of clove oil in inh...
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| Natura: | Articolo |
| Lingua: | English |
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AMG Transcend Association
2022
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| Accesso online: | https://repository.ugm.ac.id/283365/1/Clove-oil-has-the-activity-to-inhibit-middle-maturation-and-degradation-phase-of-candida-tropicalis-biofilm-formationBiointerface-Research-in-Applied-Chemistry.pdf |
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| author | Hamzah, Hasyrul Yudhawan, Indra Rasdianah, Nur Setyowati, Endang Nandini, Erika Pratiwi, Sylvia Utami Tunjung |
| author_facet | Hamzah, Hasyrul Yudhawan, Indra Rasdianah, Nur Setyowati, Endang Nandini, Erika Pratiwi, Sylvia Utami Tunjung |
| author_sort | Hamzah, Hasyrul |
| collection | UGM |
| description | Clove oil is one of the natural antibacterial ingredients that is easily obtained because of its abundant amounts in nature. Various researches have been conducted, but the antibiofilm activity against Candida tropicalis has never been done. This study evaluates the effectiveness of clove oil in inhibiting and degrading C. tropicalis JFM 1541 biofilm activity. The research was conducted using the microtiter broth method. The antibiofilm activity was determined as the minimum biofilm inhibitory concentration (MBIC50), the minimum value of biofilm eradication concentration (MBEC50). Antibiofilm mechanism was elucidated using scanning electron microscopy (SEM). Statistical analyzes were performed using ANOVA (p <0.05). Showed that clove oil could inhibit biofilm formation at the middle phase by 65% (65.21 ± 0.01) and at the maturation phase by 56% (56.11 ± 0.01). Clove oil with a concentration of 1% v/v has been shown to have activity in degrading 41% of C. tropicalis biofilms (41.87 ± 0.01). SEM shows that clove oil can cause damage in the extracellular polymeric matrix (EPS) of C. tropicalis biofilm. In conclusion, clove oil acts as a potential antibiofilm activity against C. tropicalis (compared to nystatin as control drugs) and further developed a new antibiofilm agent |
| first_indexed | 2024-03-14T00:07:40Z |
| format | Article |
| id | oai:generic.eprints.org:283365 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:07:40Z |
| publishDate | 2022 |
| publisher | AMG Transcend Association |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2833652023-11-20T07:42:02Z https://repository.ugm.ac.id/283365/ Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation Hamzah, Hasyrul Yudhawan, Indra Rasdianah, Nur Setyowati, Endang Nandini, Erika Pratiwi, Sylvia Utami Tunjung Pharmacology and Pharmaceutical Sciences Pharmaceutical Sciences Clove oil is one of the natural antibacterial ingredients that is easily obtained because of its abundant amounts in nature. Various researches have been conducted, but the antibiofilm activity against Candida tropicalis has never been done. This study evaluates the effectiveness of clove oil in inhibiting and degrading C. tropicalis JFM 1541 biofilm activity. The research was conducted using the microtiter broth method. The antibiofilm activity was determined as the minimum biofilm inhibitory concentration (MBIC50), the minimum value of biofilm eradication concentration (MBEC50). Antibiofilm mechanism was elucidated using scanning electron microscopy (SEM). Statistical analyzes were performed using ANOVA (p <0.05). Showed that clove oil could inhibit biofilm formation at the middle phase by 65% (65.21 ± 0.01) and at the maturation phase by 56% (56.11 ± 0.01). Clove oil with a concentration of 1% v/v has been shown to have activity in degrading 41% of C. tropicalis biofilms (41.87 ± 0.01). SEM shows that clove oil can cause damage in the extracellular polymeric matrix (EPS) of C. tropicalis biofilm. In conclusion, clove oil acts as a potential antibiofilm activity against C. tropicalis (compared to nystatin as control drugs) and further developed a new antibiofilm agent AMG Transcend Association 2022-04 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283365/1/Clove-oil-has-the-activity-to-inhibit-middle-maturation-and-degradation-phase-of-candida-tropicalis-biofilm-formationBiointerface-Research-in-Applied-Chemistry.pdf Hamzah, Hasyrul and Yudhawan, Indra and Rasdianah, Nur and Setyowati, Endang and Nandini, Erika and Pratiwi, Sylvia Utami Tunjung (2022) Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation. Biointerface Research in Applied Chemistry, 12 (2). pp. 1507-1519. ISSN 20695837 https://www.scopus.com/record/display.uri?eid=2-s2.0-85110464202&doi=10.33263%2fBRIAC122.15071519&origin=inward&txGid=6f574e01982761735bd98e8d4d81cb6d https://doi.org/10.33263/BRIAC122.15071519 |
| spellingShingle | Pharmacology and Pharmaceutical Sciences Pharmaceutical Sciences Hamzah, Hasyrul Yudhawan, Indra Rasdianah, Nur Setyowati, Endang Nandini, Erika Pratiwi, Sylvia Utami Tunjung Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation |
| title | Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation |
| title_full | Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation |
| title_fullStr | Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation |
| title_full_unstemmed | Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation |
| title_short | Clove Oil Has the Activity to Inhibit Middle, Maturation and Degradation Phase of Candida Tropicalis Biofilm Formation |
| title_sort | clove oil has the activity to inhibit middle maturation and degradation phase of candida tropicalis biofilm formation |
| topic | Pharmacology and Pharmaceutical Sciences Pharmaceutical Sciences |
| url | https://repository.ugm.ac.id/283365/1/Clove-oil-has-the-activity-to-inhibit-middle-maturation-and-degradation-phase-of-candida-tropicalis-biofilm-formationBiointerface-Research-in-Applied-Chemistry.pdf |
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