Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma
OBJECTIVE: Despite apoptosis processes being conserved, cancer cells have developed mechanisms to inhibit apoptosis by altering anti-apoptotic molecules or inactivating pro-apoptotic. The aim of this study was to determine the palmitic acid of Musa paradisiaca var. sapientum (L) Kunz (MP) stem extra...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Verduci Editore s.r.l
2022
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/283406/1/HS%20Budi_Palmitic%20acid%20of%20musa%20paradisiaca.pdf |
| _version_ | 1826050640181198848 |
|---|---|
| author | Budi, Hendrik Setia Anitasari, S. Ulfa, M. N. Setiabudi, M. A. Ramasamy, R. Wu, C.-Z. Shen, Y.-K. |
| author_facet | Budi, Hendrik Setia Anitasari, S. Ulfa, M. N. Setiabudi, M. A. Ramasamy, R. Wu, C.-Z. Shen, Y.-K. |
| author_sort | Budi, Hendrik Setia |
| collection | UGM |
| description | OBJECTIVE: Despite apoptosis processes being conserved, cancer cells have developed mechanisms to inhibit apoptosis by altering anti-apoptotic molecules or inactivating pro-apoptotic. The aim of this study was to determine the palmitic acid of Musa paradisiaca var. sapientum (L) Kunz (MP) stem extracts against human oral squamous cell carcinoma (hOSCC) through caspase-3. MATERIALS AND METHODS: Ethanol and ethyl acetate extracts of MP stem were analyzed by gas chromatography-mass spectrometry (GC-MS). Computerized models of chemically active compounds were used to predict anticancer activity. Cytotoxicity was evaluated in Artemia salina Leach and hOSCC (OM-1) culture at concentrations 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 μg/mL respectively. The expression level of caspase-3 on hOSCC was measured by enzyme- linked immunoassay (ELISA). RESULTS: We found seven chemically active compounds in the ethanol extract and 15 compounds in the ethyl acetate extract of MP stem. The major component was hexadecanoic acid of palmitic acid derivates, and this was predicted to have anticancer activities as apoptosis through caspase-3 stimulants. However, cytotoxicity effects against hOSCC culture were assessed by values of the 50% inhibitory concentration (IC50) of 15.00 μg/mL for the ethanol extract, and an IC50 of 10.61 μg/mL for the ethyl acetate. There was a significant increase of caspase-3 level on treatment groups compared to control. CONCLUSIONS: Hexadecanoic acid of MP stem extracts has anticancer activity by inhibiting cell growth of hOSCC culture through caspase-3 stimulants. |
| first_indexed | 2024-03-14T00:07:46Z |
| format | Article |
| id | oai:generic.eprints.org:283406 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:07:46Z |
| publishDate | 2022 |
| publisher | Verduci Editore s.r.l |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2834062023-11-21T03:23:29Z https://repository.ugm.ac.id/283406/ Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma Budi, Hendrik Setia Anitasari, S. Ulfa, M. N. Setiabudi, M. A. Ramasamy, R. Wu, C.-Z. Shen, Y.-K. Oncology and Carcinogenesis Cancer Therapy (excl. Chemotherapy and Radiation Therapy) OBJECTIVE: Despite apoptosis processes being conserved, cancer cells have developed mechanisms to inhibit apoptosis by altering anti-apoptotic molecules or inactivating pro-apoptotic. The aim of this study was to determine the palmitic acid of Musa paradisiaca var. sapientum (L) Kunz (MP) stem extracts against human oral squamous cell carcinoma (hOSCC) through caspase-3. MATERIALS AND METHODS: Ethanol and ethyl acetate extracts of MP stem were analyzed by gas chromatography-mass spectrometry (GC-MS). Computerized models of chemically active compounds were used to predict anticancer activity. Cytotoxicity was evaluated in Artemia salina Leach and hOSCC (OM-1) culture at concentrations 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 μg/mL respectively. The expression level of caspase-3 on hOSCC was measured by enzyme- linked immunoassay (ELISA). RESULTS: We found seven chemically active compounds in the ethanol extract and 15 compounds in the ethyl acetate extract of MP stem. The major component was hexadecanoic acid of palmitic acid derivates, and this was predicted to have anticancer activities as apoptosis through caspase-3 stimulants. However, cytotoxicity effects against hOSCC culture were assessed by values of the 50% inhibitory concentration (IC50) of 15.00 μg/mL for the ethanol extract, and an IC50 of 10.61 μg/mL for the ethyl acetate. There was a significant increase of caspase-3 level on treatment groups compared to control. CONCLUSIONS: Hexadecanoic acid of MP stem extracts has anticancer activity by inhibiting cell growth of hOSCC culture through caspase-3 stimulants. Verduci Editore s.r.l 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283406/1/HS%20Budi_Palmitic%20acid%20of%20musa%20paradisiaca.pdf Budi, Hendrik Setia and Anitasari, S. and Ulfa, M. N. and Setiabudi, M. A. and Ramasamy, R. and Wu, C.-Z. and Shen, Y.-K. (2022) Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma. European Review for Medical and Pharmacological Sciences, 26 (19). pp. 7099-7114. ISSN 11283602 https://repository.unair.ac.id/124127/ 10.26355/eurrev_202210_29895 |
| spellingShingle | Oncology and Carcinogenesis Cancer Therapy (excl. Chemotherapy and Radiation Therapy) Budi, Hendrik Setia Anitasari, S. Ulfa, M. N. Setiabudi, M. A. Ramasamy, R. Wu, C.-Z. Shen, Y.-K. Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma |
| title | Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma |
| title_full | Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma |
| title_fullStr | Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma |
| title_full_unstemmed | Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma |
| title_short | Palmitic acid of Musa Paradisiaca induces apoptosis through caspase-3 in human oral squamous cell carcinoma |
| title_sort | palmitic acid of musa paradisiaca induces apoptosis through caspase 3 in human oral squamous cell carcinoma |
| topic | Oncology and Carcinogenesis Cancer Therapy (excl. Chemotherapy and Radiation Therapy) |
| url | https://repository.ugm.ac.id/283406/1/HS%20Budi_Palmitic%20acid%20of%20musa%20paradisiaca.pdf |
| work_keys_str_mv | AT budihendriksetia palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma AT anitasaris palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma AT ulfamn palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma AT setiabudima palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma AT ramasamyr palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma AT wucz palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma AT shenyk palmiticacidofmusaparadisiacainducesapoptosisthroughcaspase3inhumanoralsquamouscellcarcinoma |