Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats
Background: In this study, we examined the anti-diabetic activity of standardised extracts of Tithonia diversifolia (Hemsley) A Gray (T. diversifolia) leaves for their effects on insulin resistance and mitochondrial DNA (mtDNA) copy number. Methods: T. diversifolia leaves were extracted using an ult...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Sains Malaysia
2022
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| Online Access: | https://repository.ugm.ac.id/283581/1/Rochmy%20Istikharah_Standardised%20ethanol.pdf |
| _version_ | 1826050651389427712 |
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| author | Istikharah, Rochmy Nugrahaningsih, Dwi Aris Agung Sadewa, Ahmad Hamim Wahyuningsih, Mae Sri Hartati |
| author_facet | Istikharah, Rochmy Nugrahaningsih, Dwi Aris Agung Sadewa, Ahmad Hamim Wahyuningsih, Mae Sri Hartati |
| author_sort | Istikharah, Rochmy |
| collection | UGM |
| description | Background: In this study, we examined the anti-diabetic activity of standardised extracts of Tithonia diversifolia (Hemsley) A Gray (T. diversifolia) leaves for their effects on insulin resistance and mitochondrial DNA (mtDNA) copy number. Methods: T. diversifolia leaves were extracted using an ultrasound-assisted method and standardised using Tagitinin C. There were six groups: i) normal control; ii) diabetic group; iii) metformin group (300 mg/kg) and iv) groups treated with three different doses of extract (50 mg/kg, 100 mg/kg and 150 mg/kg). Blood samples were taken before and after 28 days of treatment for fasting plasma glucose (FPG) and insulin analysis, which were used for a Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) calculation. The soleus and gastrocnemius muscles were harvested after 28 days of treatment for the measurement of mtDNA copy number. Results: The results showed an improvement in blood glucose levels and HOMA-IR scores in all treatment groups. The results of mtDNA copy number analysis also revealed significant improvement with the highest number observed at an extract dose of 100 mg/kg in which the mtDNA copy number increased up to 3 times in the soleus muscles (P < 0.001). Conclusion: T. diversifolia extract has the potential to be used as an anti-diabetic agent that improves insulin resistance, possibly by increasing mtDNA content. |
| first_indexed | 2024-03-14T00:07:57Z |
| format | Article |
| id | oai:generic.eprints.org:283581 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:07:57Z |
| publishDate | 2022 |
| publisher | Penerbit Universiti Sains Malaysia |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2835812023-11-21T06:05:18Z https://repository.ugm.ac.id/283581/ Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats Istikharah, Rochmy Nugrahaningsih, Dwi Aris Agung Sadewa, Ahmad Hamim Wahyuningsih, Mae Sri Hartati Pharmaceutical Sciences Pharmacology and Pharmaceutical Sciences Background: In this study, we examined the anti-diabetic activity of standardised extracts of Tithonia diversifolia (Hemsley) A Gray (T. diversifolia) leaves for their effects on insulin resistance and mitochondrial DNA (mtDNA) copy number. Methods: T. diversifolia leaves were extracted using an ultrasound-assisted method and standardised using Tagitinin C. There were six groups: i) normal control; ii) diabetic group; iii) metformin group (300 mg/kg) and iv) groups treated with three different doses of extract (50 mg/kg, 100 mg/kg and 150 mg/kg). Blood samples were taken before and after 28 days of treatment for fasting plasma glucose (FPG) and insulin analysis, which were used for a Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) calculation. The soleus and gastrocnemius muscles were harvested after 28 days of treatment for the measurement of mtDNA copy number. Results: The results showed an improvement in blood glucose levels and HOMA-IR scores in all treatment groups. The results of mtDNA copy number analysis also revealed significant improvement with the highest number observed at an extract dose of 100 mg/kg in which the mtDNA copy number increased up to 3 times in the soleus muscles (P < 0.001). Conclusion: T. diversifolia extract has the potential to be used as an anti-diabetic agent that improves insulin resistance, possibly by increasing mtDNA content. Penerbit Universiti Sains Malaysia 2022-06 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283581/1/Rochmy%20Istikharah_Standardised%20ethanol.pdf Istikharah, Rochmy and Nugrahaningsih, Dwi Aris Agung and Sadewa, Ahmad Hamim and Wahyuningsih, Mae Sri Hartati (2022) Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats. Malaysian Journal Medical Sciences, 29 (3). pp. 43-53. ISSN 1394195X https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249416/ 10.21315/mjms2022.29.3.5 |
| spellingShingle | Pharmaceutical Sciences Pharmacology and Pharmaceutical Sciences Istikharah, Rochmy Nugrahaningsih, Dwi Aris Agung Sadewa, Ahmad Hamim Wahyuningsih, Mae Sri Hartati Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats |
| title | Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats |
| title_full | Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats |
| title_fullStr | Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats |
| title_full_unstemmed | Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats |
| title_short | Standardised Ethanol Extract of Tithonia diversifolia (Hemsley) A Gray Leaves Improve Insulin Sensitivity and Increase Mitochondrial DNA Copy Numbers in Skeletal Muscles of Streptozotocin-Nicotinamide-Induced Rats |
| title_sort | standardised ethanol extract of tithonia diversifolia hemsley a gray leaves improve insulin sensitivity and increase mitochondrial dna copy numbers in skeletal muscles of streptozotocin nicotinamide induced rats |
| topic | Pharmaceutical Sciences Pharmacology and Pharmaceutical Sciences |
| url | https://repository.ugm.ac.id/283581/1/Rochmy%20Istikharah_Standardised%20ethanol.pdf |
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