Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication

Lard (LD) has a similarity with chicken fat (CF) in terms of physicochemical characteristics especially fatty acid composition and FTIR spectra, therefore, analysis of lard in a binary mixture with CF is challenging. This study aimed to employ FTIR spectra in normal and derivative modes in combinati...

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Main Authors: Irnawati, Irnawati, Putri, R. N., Lestari, L. A., Rohman, A.
Format: Article
Language:English
Published: Rynnye Lyan Resources 2022
Subjects:
Online Access:https://repository.ugm.ac.id/283687/1/40_Quantitative%20analysis%20and%20discrimination%20of%20lard%20in%20chicken%20fat%20using%20FTIR%20spectroscopy%20and%20chemometrics%20for%20halal%20authentication.pdf
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author Irnawati, Irnawati
Putri, R. N.
Lestari, L. A.
Rohman, A.
author_facet Irnawati, Irnawati
Putri, R. N.
Lestari, L. A.
Rohman, A.
author_sort Irnawati, Irnawati
collection UGM
description Lard (LD) has a similarity with chicken fat (CF) in terms of physicochemical characteristics especially fatty acid composition and FTIR spectra, therefore, analysis of lard in a binary mixture with CF is challenging. This study aimed to employ FTIR spectra in normal and derivative modes in combination with multivariate calibrations for quantitative analysis of LD and CF in the binary mixture and to discriminate pure LD and LD in the mixture with CF using discriminant analysis (DA). LD and CF were prepared randomly at a certain concentration to be used as calibration and validation sets, respectively. All mixtures were scanned using an FTIR spectrophotometer at 4000-650 cm-1 in absorbance mode. Some wavenumbers, FTIR spectral modes and multivariate calibrations were optimized to provide high accuracy and precision as indicated by the high value of coefficient determination (R2) and low values of errors in calibration and validation. The results revealed that FTIR spectra using normal spectra at combined wavenumbers of 3100-2750 and 1500-660 cm-1 with R2 for the relationship between actual and FTIR predicted values of > 0.99 in calibration and validation with an error in calibration of 0.008 and error validation of 0.032. DA could discriminate LD and LD in the mixture with CF with an accuracy level of 100. FTIR spectroscopy in combination with chemometrics offered a reliable method for quantitative analysis and discrimination of LD in a binary mixture with CF. © 2022, Rynnye Lyan Resources. All rights reserved.
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spelling oai:generic.eprints.org:2836872023-11-22T01:40:50Z https://repository.ugm.ac.id/283687/ Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication Irnawati, Irnawati Putri, R. N. Lestari, L. A. Rohman, A. Analytical Chemistry Lard (LD) has a similarity with chicken fat (CF) in terms of physicochemical characteristics especially fatty acid composition and FTIR spectra, therefore, analysis of lard in a binary mixture with CF is challenging. This study aimed to employ FTIR spectra in normal and derivative modes in combination with multivariate calibrations for quantitative analysis of LD and CF in the binary mixture and to discriminate pure LD and LD in the mixture with CF using discriminant analysis (DA). LD and CF were prepared randomly at a certain concentration to be used as calibration and validation sets, respectively. All mixtures were scanned using an FTIR spectrophotometer at 4000-650 cm-1 in absorbance mode. Some wavenumbers, FTIR spectral modes and multivariate calibrations were optimized to provide high accuracy and precision as indicated by the high value of coefficient determination (R2) and low values of errors in calibration and validation. The results revealed that FTIR spectra using normal spectra at combined wavenumbers of 3100-2750 and 1500-660 cm-1 with R2 for the relationship between actual and FTIR predicted values of > 0.99 in calibration and validation with an error in calibration of 0.008 and error validation of 0.032. DA could discriminate LD and LD in the mixture with CF with an accuracy level of 100. FTIR spectroscopy in combination with chemometrics offered a reliable method for quantitative analysis and discrimination of LD in a binary mixture with CF. © 2022, Rynnye Lyan Resources. All rights reserved. Rynnye Lyan Resources 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283687/1/40_Quantitative%20analysis%20and%20discrimination%20of%20lard%20in%20chicken%20fat%20using%20FTIR%20spectroscopy%20and%20chemometrics%20for%20halal%20authentication.pdf Irnawati, Irnawati and Putri, R. N. and Lestari, L. A. and Rohman, A. (2022) Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication. Food Research, 6 (4). pp. 211-217. ISSN 25502166 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138069785&doi=10.26656%2ffr.2017.6%284%29.459&partnerID=40&md5=bdb20b74c4438f50bd5930d119963308
spellingShingle Analytical Chemistry
Irnawati, Irnawati
Putri, R. N.
Lestari, L. A.
Rohman, A.
Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication
title Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication
title_full Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication
title_fullStr Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication
title_full_unstemmed Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication
title_short Quantitative analysis and discrimination of lard in chicken fat using FTIR spectroscopy and chemometrics for halal authentication
title_sort quantitative analysis and discrimination of lard in chicken fat using ftir spectroscopy and chemometrics for halal authentication
topic Analytical Chemistry
url https://repository.ugm.ac.id/283687/1/40_Quantitative%20analysis%20and%20discrimination%20of%20lard%20in%20chicken%20fat%20using%20FTIR%20spectroscopy%20and%20chemometrics%20for%20halal%20authentication.pdf
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AT lestarila quantitativeanalysisanddiscriminationoflardinchickenfatusingftirspectroscopyandchemometricsforhalalauthentication
AT rohmana quantitativeanalysisanddiscriminationoflardinchickenfatusingftirspectroscopyandchemometricsforhalalauthentication