Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis
Biofilm-forming fungi, Candida albicans, are currently a serious problem in infectious disease cases. Soil bacteria Streptomyces sp. GMR22 have a large genome size and antifungal metabolites against C. albicans, but its potential antibiofilm activity is not clearly defined. The aims of this study we...
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| Format: | Article |
| Language: | English |
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Elsevier Ltd
2022
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| Online Access: | https://repository.ugm.ac.id/283926/1/1-s2.0-S2405844022006211-main.pdf |
| _version_ | 1826050713478758400 |
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| author | Nirwati, Hera Damayanti, Ema Sholikhah, Eti Nurwening Mustofa, Mustofa Widada, Jaka |
| author_facet | Nirwati, Hera Damayanti, Ema Sholikhah, Eti Nurwening Mustofa, Mustofa Widada, Jaka |
| author_sort | Nirwati, Hera |
| collection | UGM |
| description | Biofilm-forming fungi, Candida albicans, are currently a serious problem in infectious disease cases. Soil bacteria Streptomyces sp. GMR22 have a large genome size and antifungal metabolites against C. albicans, but its potential antibiofilm activity is not clearly defined. The aims of this study were to determine the antibiofilm activity of GMR22 against C. albicans, identify the main constituents of active extracts, and investigate the biosynthesis gene clusters encoding the enzymes related to metabolism pathways. Antifungal and antibiofilm measurements were performed using in vitro assays on C. albicans ATCC 10231. Main constituents of active extracts were analyzed using untargeted Liquid Chromatography tandem High-Resolution Mass Spectrometry (LC-HRMS). RAST software was applied to investigate the gene clusters of the biosynthesis pathways based on whole genome sequences. Chloroform extract of GMR22 has antifungal and antibiofilm properties at 13–420 μg/mL with palmitic acid (C16H32O2, 273.27028 Da), a saturated fatty acid as a major constituent (42.74). Streptomyces sp. GMR22 has 53 subsystems related to fatty acids biosynthesis (Fab) FAS II. The Kyoto Encyclopedia of Gene and Genome map of Fab revealed 10 of 21 (47.6%) gene clusters encode enzymes related to Fab. There were six gene clusters encoding the enzymes related to the hexadecenoic acid (palmitic acid) biosynthesis pathways: 6.4.12; FabD, FabH, FabF, FabG, FabI and 1.14.192. Each enzyme was encoded by 3–14 genes. These results confirmed that soil Streptomyces sp. GMR22 bacterium has remarkable biotechnological potential by producing fatty acids which are mostly palmitic acid as an active antibiofilm agent against C. albicans. |
| first_indexed | 2024-03-14T00:08:56Z |
| format | Article |
| id | oai:generic.eprints.org:283926 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:08:56Z |
| publishDate | 2022 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2839262023-11-24T02:46:12Z https://repository.ugm.ac.id/283926/ Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis Nirwati, Hera Damayanti, Ema Sholikhah, Eti Nurwening Mustofa, Mustofa Widada, Jaka Medical Bacteriology Pharmacology and Pharmaceutical Sciences Biofilm-forming fungi, Candida albicans, are currently a serious problem in infectious disease cases. Soil bacteria Streptomyces sp. GMR22 have a large genome size and antifungal metabolites against C. albicans, but its potential antibiofilm activity is not clearly defined. The aims of this study were to determine the antibiofilm activity of GMR22 against C. albicans, identify the main constituents of active extracts, and investigate the biosynthesis gene clusters encoding the enzymes related to metabolism pathways. Antifungal and antibiofilm measurements were performed using in vitro assays on C. albicans ATCC 10231. Main constituents of active extracts were analyzed using untargeted Liquid Chromatography tandem High-Resolution Mass Spectrometry (LC-HRMS). RAST software was applied to investigate the gene clusters of the biosynthesis pathways based on whole genome sequences. Chloroform extract of GMR22 has antifungal and antibiofilm properties at 13–420 μg/mL with palmitic acid (C16H32O2, 273.27028 Da), a saturated fatty acid as a major constituent (42.74). Streptomyces sp. GMR22 has 53 subsystems related to fatty acids biosynthesis (Fab) FAS II. The Kyoto Encyclopedia of Gene and Genome map of Fab revealed 10 of 21 (47.6%) gene clusters encode enzymes related to Fab. There were six gene clusters encoding the enzymes related to the hexadecenoic acid (palmitic acid) biosynthesis pathways: 6.4.12; FabD, FabH, FabF, FabG, FabI and 1.14.192. Each enzyme was encoded by 3–14 genes. These results confirmed that soil Streptomyces sp. GMR22 bacterium has remarkable biotechnological potential by producing fatty acids which are mostly palmitic acid as an active antibiofilm agent against C. albicans. Elsevier Ltd 2022-04-21 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283926/1/1-s2.0-S2405844022006211-main.pdf Nirwati, Hera and Damayanti, Ema and Sholikhah, Eti Nurwening and Mustofa, Mustofa and Widada, Jaka (2022) Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis. Heliyon, 8 (4). ISSN 2405-8440 https://www.sciencedirect.com/science/article/pii/S2405844022006211 10.1016/j.heliyon.2022.e09333 |
| spellingShingle | Medical Bacteriology Pharmacology and Pharmaceutical Sciences Nirwati, Hera Damayanti, Ema Sholikhah, Eti Nurwening Mustofa, Mustofa Widada, Jaka Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis |
| title | Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis |
| title_full | Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis |
| title_fullStr | Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis |
| title_full_unstemmed | Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis |
| title_short | Soil-derived Streptomyces sp. GMR22 producing antibiofilm activity against Candida albicans: bioassay, untargeted LC-HRMS, and gene cluster analysis |
| title_sort | soil derived streptomyces sp gmr22 producing antibiofilm activity against candida albicans bioassay untargeted lc hrms and gene cluster analysis |
| topic | Medical Bacteriology Pharmacology and Pharmaceutical Sciences |
| url | https://repository.ugm.ac.id/283926/1/1-s2.0-S2405844022006211-main.pdf |
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