Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant
Buccal mucosae are considered as a site for vaccine delivery since they are relatively abundant with antigen-presenting dendritic cells, mainly Langerhans cells. In this study, we formulated carbonated hydroxy apatite (CHA) with ovalbumin (OVA) (denoted as CHA-OVA), incorporated it into bilayer bucc...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Japanese Society for Dental Materials and Devices
2022
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/283941/1/Anggraeni_KG.pdf |
| _version_ | 1797037767136378880 |
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| author | Anggraeni, Rahmi Ana, Ika Dewi Agustina, Dewi Martien, Ronny |
| author_facet | Anggraeni, Rahmi Ana, Ika Dewi Agustina, Dewi Martien, Ronny |
| author_sort | Anggraeni, Rahmi |
| collection | UGM |
| description | Buccal mucosae are considered as a site for vaccine delivery since they are relatively abundant with antigen-presenting dendritic cells, mainly Langerhans cells. In this study, we formulated carbonated hydroxy apatite (CHA) with ovalbumin (OVA) (denoted as CHA-OVA), incorporated it into bilayer buccal membrane to form hydrogel films containing CHA-OVA complex for vaccination via buccal mucosae. Ethylcellulose blend with polyethylene glycol 400 were used as impermeable backing layer. Physical properties of all tested buccal membranes were found suitable for mucosal application. In vitro and ex vivo release study showed there was no burst release of OVA found from all tested formula. From the in vivo examination, rabbit buccal mucosae vaccinated by mucoadhesive membranes containing CHA-OVA complex demonstrated mucosal specific antibody induction, represented the potential of CHA as a candidate of needle-free vaccine adjuvant. Future research is awaiting to investigate proper CHA crystallinity in complex with protein against targeted diseases. |
| first_indexed | 2024-03-14T00:08:59Z |
| format | Article |
| id | oai:generic.eprints.org:283941 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:08:59Z |
| publishDate | 2022 |
| publisher | The Japanese Society for Dental Materials and Devices |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2839412023-11-24T07:38:22Z https://repository.ugm.ac.id/283941/ Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant Anggraeni, Rahmi Ana, Ika Dewi Agustina, Dewi Martien, Ronny Dentistry Buccal mucosae are considered as a site for vaccine delivery since they are relatively abundant with antigen-presenting dendritic cells, mainly Langerhans cells. In this study, we formulated carbonated hydroxy apatite (CHA) with ovalbumin (OVA) (denoted as CHA-OVA), incorporated it into bilayer buccal membrane to form hydrogel films containing CHA-OVA complex for vaccination via buccal mucosae. Ethylcellulose blend with polyethylene glycol 400 were used as impermeable backing layer. Physical properties of all tested buccal membranes were found suitable for mucosal application. In vitro and ex vivo release study showed there was no burst release of OVA found from all tested formula. From the in vivo examination, rabbit buccal mucosae vaccinated by mucoadhesive membranes containing CHA-OVA complex demonstrated mucosal specific antibody induction, represented the potential of CHA as a candidate of needle-free vaccine adjuvant. Future research is awaiting to investigate proper CHA crystallinity in complex with protein against targeted diseases. The Japanese Society for Dental Materials and Devices 2022 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/283941/1/Anggraeni_KG.pdf Anggraeni, Rahmi and Ana, Ika Dewi and Agustina, Dewi and Martien, Ronny (2022) Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant. Dental Materials Journal, 41 (5). pp. 710-723. ISSN 1881-1361 https://www.jstage.jst.go.jp/article/dmj/41/5/41_2021-254/_article https://doi.org/10.4012/dmj.2021-254 |
| spellingShingle | Dentistry Anggraeni, Rahmi Ana, Ika Dewi Agustina, Dewi Martien, Ronny Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| title | Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| title_full | Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| title_fullStr | Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| title_full_unstemmed | Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| title_short | Induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| title_sort | induction of protein specific antibody by carbonated hydroxy apatite as a candidate for mucosal vaccine adjuvant |
| topic | Dentistry |
| url | https://repository.ugm.ac.id/283941/1/Anggraeni_KG.pdf |
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