Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey
Ankara Province with its sizeable stone fruits outputs and its geographic location in the middle of Turkey might be one of the diversity centers of plum pox virus (PPV) in the country, yet the epidemiological data from there were rather limited. Multi-year extensive surveillance in all 25 districts...
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| Format: | Article |
| Language: | English |
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Springer Nature
2022
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| Online Access: | https://repository.ugm.ac.id/284037/1/Comprehensive-surveillance-and-population-study-on-plum-pox-virus-in-Ankara-Province-of-TurkeyJournal-of-Plant-Diseases-and-Protection.pdf |
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| author | Coşkan, Sevgi Morca, Ali Ferhan Akbaş, Birol Çelik, Ali Santosa, Adyatma Irawan |
| author_facet | Coşkan, Sevgi Morca, Ali Ferhan Akbaş, Birol Çelik, Ali Santosa, Adyatma Irawan |
| author_sort | Coşkan, Sevgi |
| collection | UGM |
| description | Ankara Province with its sizeable stone fruits outputs and its geographic location in the middle of Turkey might be one of the diversity centers of plum pox virus (PPV) in the country, yet the epidemiological data from there were rather limited. Multi-year extensive surveillance in all 25 districts of Ankara sampled 8131 Prunus spp. plants to be tested against PPV. DAS-ELISA detected 609 PPV positive samples (7.49%), which were all also confirmed by RT-PCR using two primer pairs to amplify P3-6K1-CI and partial NIb-CP regions. The partial genomes of 80 isolates were sequenced, then the sequences were subjected to phylogenetic and population analyses together with 170 isolates which complete genome sequences available in NCBI GenBank. The phylogenetic study showed that 73 new isolates were PPV-T, six isolates were PPV-M, and one isolate was PPV-D. The subsequent population study confirmed the interesting features of PPV with very high genetic diversities at the species level, thus it should be divided into strains that each retained much lower divergence among isolates. The obtained data also could provide new evidence to separate M, and MIs isolates into two distinct strains. Although previous studies suggested that Turkish isolates have been endemic since a long time ago in the country, the results of demographic analyses of the present study indicated that the expansions of Turkey and Ankara populations were recent, driven by relatively new mutations in their genome. |
| first_indexed | 2024-03-14T00:09:16Z |
| format | Article |
| id | oai:generic.eprints.org:284037 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:09:16Z |
| publishDate | 2022 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2840372023-11-27T06:49:18Z https://repository.ugm.ac.id/284037/ Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey Coşkan, Sevgi Morca, Ali Ferhan Akbaş, Birol Çelik, Ali Santosa, Adyatma Irawan Other Agricultural and Veterinary Sciences Ankara Province with its sizeable stone fruits outputs and its geographic location in the middle of Turkey might be one of the diversity centers of plum pox virus (PPV) in the country, yet the epidemiological data from there were rather limited. Multi-year extensive surveillance in all 25 districts of Ankara sampled 8131 Prunus spp. plants to be tested against PPV. DAS-ELISA detected 609 PPV positive samples (7.49%), which were all also confirmed by RT-PCR using two primer pairs to amplify P3-6K1-CI and partial NIb-CP regions. The partial genomes of 80 isolates were sequenced, then the sequences were subjected to phylogenetic and population analyses together with 170 isolates which complete genome sequences available in NCBI GenBank. The phylogenetic study showed that 73 new isolates were PPV-T, six isolates were PPV-M, and one isolate was PPV-D. The subsequent population study confirmed the interesting features of PPV with very high genetic diversities at the species level, thus it should be divided into strains that each retained much lower divergence among isolates. The obtained data also could provide new evidence to separate M, and MIs isolates into two distinct strains. Although previous studies suggested that Turkish isolates have been endemic since a long time ago in the country, the results of demographic analyses of the present study indicated that the expansions of Turkey and Ankara populations were recent, driven by relatively new mutations in their genome. Springer Nature 2022-08 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/284037/1/Comprehensive-surveillance-and-population-study-on-plum-pox-virus-in-Ankara-Province-of-TurkeyJournal-of-Plant-Diseases-and-Protection.pdf Coşkan, Sevgi and Morca, Ali Ferhan and Akbaş, Birol and Çelik, Ali and Santosa, Adyatma Irawan (2022) Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey. Journal of Plant Diseases and Protection, 129 (4). pp. 981-991. ISSN 18613829 https://link.springer.com/article/10.1007/s41348-022-00597-5 https://doi.org/10.1007/s41348-022-00597-5 |
| spellingShingle | Other Agricultural and Veterinary Sciences Coşkan, Sevgi Morca, Ali Ferhan Akbaş, Birol Çelik, Ali Santosa, Adyatma Irawan Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey |
| title | Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey |
| title_full | Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey |
| title_fullStr | Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey |
| title_full_unstemmed | Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey |
| title_short | Comprehensive surveillance and population study on plum pox virus in Ankara Province of Turkey |
| title_sort | comprehensive surveillance and population study on plum pox virus in ankara province of turkey |
| topic | Other Agricultural and Veterinary Sciences |
| url | https://repository.ugm.ac.id/284037/1/Comprehensive-surveillance-and-population-study-on-plum-pox-virus-in-Ankara-Province-of-TurkeyJournal-of-Plant-Diseases-and-Protection.pdf |
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