Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance
Basundari FRA, Sulistyaningsih E, Murti RH, Nuringtyas TR. 2021. Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance. Biodiversitas 22: 3127-3135. Shallot, an Allium species with high economic value, is widely cultivated in Indonesia. The species include...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Society for Indonesian Biodiversity
2021
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/284175/1/8252-Article%20Text-45945-3-10-20210811.pdf |
| _version_ | 1797037806375141376 |
|---|---|
| author | Basundari, Fransiska Renita Anon Sulistyaningsih, Endang Murti, Rudi Hari Nuringtyas, Tri Rini |
| author_facet | Basundari, Fransiska Renita Anon Sulistyaningsih, Endang Murti, Rudi Hari Nuringtyas, Tri Rini |
| author_sort | Basundari, Fransiska Renita Anon |
| collection | UGM |
| description | Basundari FRA, Sulistyaningsih E, Murti RH, Nuringtyas TR. 2021. Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance. Biodiversitas 22: 3127-3135. Shallot, an Allium species with high economic value, is widely cultivated in Indonesia. The species includes numerous cultivars with unique characteristics, such as harvesting time, yield, taste, odor, and bulb color. Tuk Tuk and Trisula, the cultivars used in this experiment, have different bulb colors. Tuk Tuk�s bulb color is light red, while that of Trisula is dark red. This color difference may be attributed to variations in the metabolite contents of the shallot bulbs. The present experiment aimed to reveal differences in the metabolite contents of the two shallot cultivars by using Nuclear Magnetic Resonance (NMR) and then determine the specific metabolites contributing to these differences. Bulbs of Tuk Tuk and Trisula were planted in the field under the same conditions and then collected. The bulb samples were powdered, freeze-dried, and subjected to a two-phase extraction method with CDCl3 and D2O as solvents to separate the nonpolar metabolites from polar ones. The extracts were analyzed by1H-NMR, and the spectra collected were analyzed using MNOVA software and Metaboanalyst.ca. A total of 23 metabolites were successfully identified and characterized in this experiment. The contents of eight of these metabolites, namely, sucrose, glutamine, citric acid, choline, methiin, propiin, threonine, and formic acid, were significantly higher in Trisula than in Tuk Tuk. These differences may be correlated with variations in the color intensity, pungency, and other traits of the cultivars. The results demonstrate that NMR metabolite profiling could effectively differentiate metabolite profile variations among shallot cultivars. © 2021, Society for Indonesian Biodiversity. All rights reserved. |
| first_indexed | 2024-03-14T00:09:35Z |
| format | Article |
| id | oai:generic.eprints.org:284175 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:09:35Z |
| publishDate | 2021 |
| publisher | Society for Indonesian Biodiversity |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2841752023-12-11T08:42:04Z https://repository.ugm.ac.id/284175/ Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance Basundari, Fransiska Renita Anon Sulistyaningsih, Endang Murti, Rudi Hari Nuringtyas, Tri Rini Biological Sciences Basundari FRA, Sulistyaningsih E, Murti RH, Nuringtyas TR. 2021. Metabolite profile of two Allium cepa L. aggregatum group cultivars by Nuclear Magnetic Resonance. Biodiversitas 22: 3127-3135. Shallot, an Allium species with high economic value, is widely cultivated in Indonesia. The species includes numerous cultivars with unique characteristics, such as harvesting time, yield, taste, odor, and bulb color. Tuk Tuk and Trisula, the cultivars used in this experiment, have different bulb colors. Tuk Tuk�s bulb color is light red, while that of Trisula is dark red. This color difference may be attributed to variations in the metabolite contents of the shallot bulbs. The present experiment aimed to reveal differences in the metabolite contents of the two shallot cultivars by using Nuclear Magnetic Resonance (NMR) and then determine the specific metabolites contributing to these differences. Bulbs of Tuk Tuk and Trisula were planted in the field under the same conditions and then collected. The bulb samples were powdered, freeze-dried, and subjected to a two-phase extraction method with CDCl3 and D2O as solvents to separate the nonpolar metabolites from polar ones. The extracts were analyzed by1H-NMR, and the spectra collected were analyzed using MNOVA software and Metaboanalyst.ca. A total of 23 metabolites were successfully identified and characterized in this experiment. The contents of eight of these metabolites, namely, sucrose, glutamine, citric acid, choline, methiin, propiin, threonine, and formic acid, were significantly higher in Trisula than in Tuk Tuk. These differences may be correlated with variations in the color intensity, pungency, and other traits of the cultivars. The results demonstrate that NMR metabolite profiling could effectively differentiate metabolite profile variations among shallot cultivars. © 2021, Society for Indonesian Biodiversity. All rights reserved. Society for Indonesian Biodiversity 2021 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/284175/1/8252-Article%20Text-45945-3-10-20210811.pdf Basundari, Fransiska Renita Anon and Sulistyaningsih, Endang and Murti, Rudi Hari and Nuringtyas, Tri Rini (2021) Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance. Biodiversitas, 22 (8). pp. 3127-3135. ISSN 2085-4722 https://smujo.id/biodiv/article/view/8252/5015 |
| spellingShingle | Biological Sciences Basundari, Fransiska Renita Anon Sulistyaningsih, Endang Murti, Rudi Hari Nuringtyas, Tri Rini Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance |
| title | Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance |
| title_full | Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance |
| title_fullStr | Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance |
| title_full_unstemmed | Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance |
| title_short | Metabolite profile of two allium cepa L. Aggregatum group cultivars by nuclear magnetic resonance |
| title_sort | metabolite profile of two allium cepa l aggregatum group cultivars by nuclear magnetic resonance |
| topic | Biological Sciences |
| url | https://repository.ugm.ac.id/284175/1/8252-Article%20Text-45945-3-10-20210811.pdf |
| work_keys_str_mv | AT basundarifransiskarenitaanon metaboliteprofileoftwoalliumcepalaggregatumgroupcultivarsbynuclearmagneticresonance AT sulistyaningsihendang metaboliteprofileoftwoalliumcepalaggregatumgroupcultivarsbynuclearmagneticresonance AT murtirudihari metaboliteprofileoftwoalliumcepalaggregatumgroupcultivarsbynuclearmagneticresonance AT nuringtyastririni metaboliteprofileoftwoalliumcepalaggregatumgroupcultivarsbynuclearmagneticresonance |