Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene
Streptomyces is one of mercury-resistant bacteria which can convert Hg2+ into nontoxic Hg0. This study aimed to identify mercury-resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small-scale gold mining (ASGM) area and clone merA gene to the cloning and expression vec...
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| Format: | Article |
| Language: | English |
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Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada
2021
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| Online Access: | https://repository.ugm.ac.id/284193/1/65989-240364-2-PB.pdf |
| _version_ | 1826050759295238144 |
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| author | Putri, Wahyu Aristyaning Rahayu, Hanum Mukti Khasanah, Anis Uswatun Sembiring, Langkah Kawaichi, Masashi Purwestri, Yekti Asih |
| author_facet | Putri, Wahyu Aristyaning Rahayu, Hanum Mukti Khasanah, Anis Uswatun Sembiring, Langkah Kawaichi, Masashi Purwestri, Yekti Asih |
| author_sort | Putri, Wahyu Aristyaning |
| collection | UGM |
| description | Streptomyces is one of mercury-resistant bacteria which can convert Hg2+ into nontoxic Hg0. This study aimed to identify mercury-resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small-scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX-5x-1 and pET-28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury-resistant Streptomyces and cloning the full-length merA gene in Indonesia. © 2021 Universitas Gadjah Mada, Research Center for Biotechnology. All rights reserved. |
| first_indexed | 2024-03-14T00:09:38Z |
| format | Article |
| id | oai:generic.eprints.org:284193 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:09:38Z |
| publishDate | 2021 |
| publisher | Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2841932023-11-29T08:27:48Z https://repository.ugm.ac.id/284193/ Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene Putri, Wahyu Aristyaning Rahayu, Hanum Mukti Khasanah, Anis Uswatun Sembiring, Langkah Kawaichi, Masashi Purwestri, Yekti Asih Genetics Molecular Evolution Biological Sciences Streptomyces is one of mercury-resistant bacteria which can convert Hg2+ into nontoxic Hg0. This study aimed to identify mercury-resistant Streptomyces present in the Cyperus rotundus rhizosphere from artisanal small-scale gold mining (ASGM) area and clone merA gene to the cloning and expression vectors. Molecular identification was conducted using 16s rRNA gene with the maximum likelihood algorithms. Results revealed that the AS1 and AS2 strains were a group of Streptomyces ardesiacus and the BR28 strain was closed to Brevibacillus agri. The AS2 merA gene was cloned to pMD20 cloning vectors, pGEX-5x-1 and pET-28c expression vectors. The transformation was successfully performed in BL21 and DH5α competent cells. The full length of the merA gene was confirmed to be 1,425 bp. This study is the first research on identifying mercury-resistant Streptomyces and cloning the full-length merA gene in Indonesia. © 2021 Universitas Gadjah Mada, Research Center for Biotechnology. All rights reserved. Research Center for Biotechnology in collaboration with the Graduate School of Universitas Gadjah Mada 2021 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/284193/1/65989-240364-2-PB.pdf Putri, Wahyu Aristyaning and Rahayu, Hanum Mukti and Khasanah, Anis Uswatun and Sembiring, Langkah and Kawaichi, Masashi and Purwestri, Yekti Asih (2021) Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene. Indonesian Journal of Biotechnology, 26 (4). pp. 206-213. ISSN 2089-2241 https://jurnal.ugm.ac.id/ijbiotech/article/view/65989/32917 |
| spellingShingle | Genetics Molecular Evolution Biological Sciences Putri, Wahyu Aristyaning Rahayu, Hanum Mukti Khasanah, Anis Uswatun Sembiring, Langkah Kawaichi, Masashi Purwestri, Yekti Asih Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene |
| title | Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene |
| title_full | Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene |
| title_fullStr | Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene |
| title_full_unstemmed | Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene |
| title_short | Identification of mercury-resistant Streptomyces isolated from Cyperus rotundus L. rhizosphere and molecular cloning of mercury (II) reductase gene |
| title_sort | identification of mercury resistant streptomyces isolated from cyperus rotundus l rhizosphere and molecular cloning of mercury ii reductase gene |
| topic | Genetics Molecular Evolution Biological Sciences |
| url | https://repository.ugm.ac.id/284193/1/65989-240364-2-PB.pdf |
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