Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture
Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of cul...
| Glavni autori: | , , , |
|---|---|
| Format: | Članak |
| Jezik: | English |
| Izdano: |
Faculty of Biology, UGM
2021
|
| Teme: | |
| Online pristup: | https://repository.ugm.ac.id/284194/1/64403-230843-2-PB.pdf |
| _version_ | 1826050759487127552 |
|---|---|
| author | Eskundari, Ratna Dewi Taryono, Taryono Indradewa, Didik Purwestri, Yekti Asih |
| author_facet | Eskundari, Ratna Dewi Taryono, Taryono Indradewa, Didik Purwestri, Yekti Asih |
| author_sort | Eskundari, Ratna Dewi |
| collection | UGM |
| description | Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea. © 2021, J. Tropical Biodiversity Biotechnology (CC BY-SA 4.0) |
| first_indexed | 2024-03-14T00:09:38Z |
| format | Article |
| id | oai:generic.eprints.org:284194 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-14T00:09:38Z |
| publishDate | 2021 |
| publisher | Faculty of Biology, UGM |
| record_format | dspace |
| spelling | oai:generic.eprints.org:2841942023-12-01T06:18:42Z https://repository.ugm.ac.id/284194/ Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture Eskundari, Ratna Dewi Taryono, Taryono Indradewa, Didik Purwestri, Yekti Asih Biological Sciences Tissue culture is an alternative choice of plant propagation either through somatic embryogenesis or in vitro organogenesis techniques. TRI2025 tea clone has been cultured successfully, however, the scientific information related to morphology, histology, and protein profile at an early event of culturing time has not been reported yet. This study aimed to determine the differences between those pathways, in the context of morphology, histology, and protein profile. The explants were the embryo axis of TRI2025 tea clone cultured on two different induction mediums; somatic embryogenesis and in vitro organogenesis induction medium. The results showed that most of the explants cultured on A medium developed to be a globular-like structure at 11-day after culture (DAC), while all explants cultured on B medium showed the initiation stage of in vitro organogenesis. Histological analysis showed meristem reconstruction at shoot apical meristem (SAM) and root apical meristem (RAM) at 11-DAC at explants cultured on B medium, while explants cultured on A medium showed callusing at 21-DAC. Protein profile analysis using SDS-PAGE showed protein bands of 54 and 81 KDa that only appeared at explants cultured on A medium start from 14-DAC, and those two protein bands thought to be a differentiator at the early stages of the two tissue culture techniques. Thus, these parameters can be used as early detection for plant tissue culture, especially in tea. © 2021, J. Tropical Biodiversity Biotechnology (CC BY-SA 4.0) Faculty of Biology, UGM 2021 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/284194/1/64403-230843-2-PB.pdf Eskundari, Ratna Dewi and Taryono, Taryono and Indradewa, Didik and Purwestri, Yekti Asih (2021) Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture. Journal of Tropical Biodiversity and Biotechnology, 6 (3). pp. 1-16. ISSN 2540-9581 (online) https://jurnal.ugm.ac.id/jtbb/article/view/64403/32249 |
| spellingShingle | Biological Sciences Eskundari, Ratna Dewi Taryono, Taryono Indradewa, Didik Purwestri, Yekti Asih Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture |
| title | Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture |
| title_full | Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture |
| title_fullStr | Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture |
| title_full_unstemmed | Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture |
| title_short | Morphological, Histological, and Protein Profiling of Tea Embryo Axis at Early Stage of Culture |
| title_sort | morphological histological and protein profiling of tea embryo axis at early stage of culture |
| topic | Biological Sciences |
| url | https://repository.ugm.ac.id/284194/1/64403-230843-2-PB.pdf |
| work_keys_str_mv | AT eskundariratnadewi morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture AT taryonotaryono morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture AT indradewadidik morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture AT purwestriyektiasih morphologicalhistologicalandproteinprofilingofteaembryoaxisatearlystageofculture |