DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES
Banana bunchy top disease (BBTD) can be caused by the infection of two different viruses, Banana bunchy top virus (BBTV) or Abaca bunchy top virus (ABTV). Both viruses can be transmitted persistently by aphid Pentalonia nigronervosa Coq. The research was conducted to detect and to differentiate the...
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[Yogyakarta] : Universitas Gadjah Mada
2010
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | Banana bunchy top disease (BBTD) can be caused by the infection of two different viruses, Banana bunchy top virus (BBTV) or Abaca bunchy top virus (ABTV). Both viruses can be transmitted persistently by aphid Pentalonia nigronervosa Coq. The research was conducted to detect and to differentiate the virus by polymerase chain reactionrestriction fragment length polymorphism (PCR - RFLP) techniques. Infected plants were collectedfrom Yogyakarta (Sleman, Yogyakarta city, Bantul, Gunung Kidul, and Kulon Progo). Nucleon Phytopure DNA Extraction Kit method was used to extract the total DNA of infectedplants. Universalprimers of CommonDNA region (S-CRF and S-CRR) and specific primers DNA-R (CI-CRF and CI-CRR) were used for PCR amplification. PCR products were analyzed by RFLP technique using the restriction enzyme of Dra 1. The results reconfirm previous reports that bunchy top disease of banana in Yogyakarta is caused by BBTV The ABTV was not detected in this present study. Based on the RFLP analysis it was concluded that BBTV collected in this study could be divided into three groups. Group 1 consisted of BBTV isolate from Sleman and Yogyakarta city with twofragments DNA of 400 and 388 bp. Group 2 consisted of isolate BBTV from Kulon Progo and Gunung Kidul with three fragments DNA of 400, 388, and 323 bp. Group 3 consisted of isolate from Bantul with two fragments DNA of 723 and 376 bp. Further study on the complete characteristics of these groups is still needed. |
| first_indexed | 2024-03-13T19:08:38Z |
| format | Article |
| id | oai:generic.eprints.org:28896 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:08:38Z |
| publishDate | 2010 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:288962014-06-18T00:23:52Z https://repository.ugm.ac.id/28896/ DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES Perpustakaan UGM, i-lib Jurnal i-lib UGM Banana bunchy top disease (BBTD) can be caused by the infection of two different viruses, Banana bunchy top virus (BBTV) or Abaca bunchy top virus (ABTV). Both viruses can be transmitted persistently by aphid Pentalonia nigronervosa Coq. The research was conducted to detect and to differentiate the virus by polymerase chain reactionrestriction fragment length polymorphism (PCR - RFLP) techniques. Infected plants were collectedfrom Yogyakarta (Sleman, Yogyakarta city, Bantul, Gunung Kidul, and Kulon Progo). Nucleon Phytopure DNA Extraction Kit method was used to extract the total DNA of infectedplants. Universalprimers of CommonDNA region (S-CRF and S-CRR) and specific primers DNA-R (CI-CRF and CI-CRR) were used for PCR amplification. PCR products were analyzed by RFLP technique using the restriction enzyme of Dra 1. The results reconfirm previous reports that bunchy top disease of banana in Yogyakarta is caused by BBTV The ABTV was not detected in this present study. Based on the RFLP analysis it was concluded that BBTV collected in this study could be divided into three groups. Group 1 consisted of BBTV isolate from Sleman and Yogyakarta city with twofragments DNA of 400 and 388 bp. Group 2 consisted of isolate BBTV from Kulon Progo and Gunung Kidul with three fragments DNA of 400, 388, and 323 bp. Group 3 consisted of isolate from Bantul with two fragments DNA of 723 and 376 bp. Further study on the complete characteristics of these groups is still needed. [Yogyakarta] : Universitas Gadjah Mada 2010 Article NonPeerReviewed Perpustakaan UGM, i-lib (2010) DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=11959 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES |
| title | DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES |
| title_full | DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES |
| title_fullStr | DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES |
| title_full_unstemmed | DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES |
| title_short | DETEKSI DAN DIFERENSIASI VIRUS KERDIL PISANG DENGAN TEKNIK PCR-RFLP = DETECTION AND DIFFERENTIATION OF BUNCHY TOP VIRUS IN BANANA BY PCR-RFLP TECHNIQUES |
| title_sort | deteksi dan diferensiasi virus kerdil pisang dengan teknik pcr rflp detection and differentiation of bunchy top virus in banana by pcr rflp techniques |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib deteksidandiferensiasiviruskerdilpisangdenganteknikpcrrflpdetectionanddifferentiationofbunchytopvirusinbananabypcrrflptechniques |