DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE
Tungro is one of rice disease caused by two different viruses (rice tungro virus=RTV) i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) that are transmitted only by green leafhopper. Tungro had become a serious problem in several rice productions centre in Indonesia. V...
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[Yogyakarta] : Universitas Gadjah Mada
2009
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| author | Perpustakaan UGM, i-lib |
| author_facet | Perpustakaan UGM, i-lib |
| author_sort | Perpustakaan UGM, i-lib |
| collection | UGM |
| description | Tungro is one of rice disease caused by two different viruses (rice tungro virus=RTV) i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) that are transmitted only by green leafhopper. Tungro had become a serious problem in several rice productions centre in Indonesia. Various components of management effort have been applied but they were inefficient in preventing the tungro disease development. Resistance variety is the most efficient component to tungro disease management. Complexity interactions of tungro disease components are mayor constraint in tungro disease management. Detection of molecular variability in rice tungro virus from several endemic areas in Indonesia were conducted by using PCR-RFLP technique. Existence of RTBV and RTSV in the infected plants collectedfrom several endemic areas were successfully detected by PCR. TheRFLP analysis with restriction enzymes BstYI and HindIII showed that there were significant difference among the RTSV originated from Java, Bali and Sulawesi.
Tungro merupakan salah satu penyakit padi yang disebabkanoleh dua virus (rice tungrovirus = RTV)yang berbeda yaitu Rice tungro bacilliform virus (RTBV) dan Rice tungro spherical virus (RTSV) yang keduanya hanya dapat ditularkan oleh wereng hijau. Tungro menjadi masalah serius di beberapa sentra produksi padi di Indonesia. Berbagai usaha pengendalian telah dilakukan tetapi belum dapat mencegah dan mengatasi perkembanganpenyakit tungro secara efisien.Varietastahan merupakankomponen pengendaliantungro yang paling efektif. Komplek sitasinteraksi penyebab penyakit tungro merupakan kendala utama dalam usaha pengendalian penyakit tungro. Melalui pendekatan molekuler telah dilakukan deteksi dan analisis keragaman virus tungro dari beberapa daerah endemis di Indonesia. Berdasarkan analisis PCR dengan spesifik primer telah berhasil dideteksi keberadaan RTBV dan RTSV dari tanaman terinfeksi yang dikoleksi dari beberapa daerah endemis. Hasil analisis RFLP dengan enzim restriksi Bstfl dan Hind III menunjukkan adanya keragaman genetik antara RTSV yang berasal dari Jawa, Bali dan Sulawesi. |
| first_indexed | 2024-03-05T23:13:24Z |
| format | Article |
| id | oai:generic.eprints.org:29016 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T19:09:18Z |
| publishDate | 2009 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
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| spelling | oai:generic.eprints.org:290162014-06-18T00:24:22Z https://repository.ugm.ac.id/29016/ DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE Perpustakaan UGM, i-lib Jurnal i-lib UGM Tungro is one of rice disease caused by two different viruses (rice tungro virus=RTV) i.e. Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV) that are transmitted only by green leafhopper. Tungro had become a serious problem in several rice productions centre in Indonesia. Various components of management effort have been applied but they were inefficient in preventing the tungro disease development. Resistance variety is the most efficient component to tungro disease management. Complexity interactions of tungro disease components are mayor constraint in tungro disease management. Detection of molecular variability in rice tungro virus from several endemic areas in Indonesia were conducted by using PCR-RFLP technique. Existence of RTBV and RTSV in the infected plants collectedfrom several endemic areas were successfully detected by PCR. TheRFLP analysis with restriction enzymes BstYI and HindIII showed that there were significant difference among the RTSV originated from Java, Bali and Sulawesi. Tungro merupakan salah satu penyakit padi yang disebabkanoleh dua virus (rice tungrovirus = RTV)yang berbeda yaitu Rice tungro bacilliform virus (RTBV) dan Rice tungro spherical virus (RTSV) yang keduanya hanya dapat ditularkan oleh wereng hijau. Tungro menjadi masalah serius di beberapa sentra produksi padi di Indonesia. Berbagai usaha pengendalian telah dilakukan tetapi belum dapat mencegah dan mengatasi perkembanganpenyakit tungro secara efisien.Varietastahan merupakankomponen pengendaliantungro yang paling efektif. Komplek sitasinteraksi penyebab penyakit tungro merupakan kendala utama dalam usaha pengendalian penyakit tungro. Melalui pendekatan molekuler telah dilakukan deteksi dan analisis keragaman virus tungro dari beberapa daerah endemis di Indonesia. Berdasarkan analisis PCR dengan spesifik primer telah berhasil dideteksi keberadaan RTBV dan RTSV dari tanaman terinfeksi yang dikoleksi dari beberapa daerah endemis. Hasil analisis RFLP dengan enzim restriksi Bstfl dan Hind III menunjukkan adanya keragaman genetik antara RTSV yang berasal dari Jawa, Bali dan Sulawesi. [Yogyakarta] : Universitas Gadjah Mada 2009 Article NonPeerReviewed Perpustakaan UGM, i-lib (2009) DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE. Jurnal i-lib UGM. http://i-lib.ugm.ac.id/jurnal/download.php?dataId=12079 |
| spellingShingle | Jurnal i-lib UGM Perpustakaan UGM, i-lib DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE |
| title | DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE |
| title_full | DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE |
| title_fullStr | DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE |
| title_full_unstemmed | DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE |
| title_short | DETEKSI KERAGAMAN VIRUS TUNGRO DARI BEBERAPA DAERAH ENDEMIS DI INDONESIA DENGAN TEKNIK PCR-RFLP = DETECTION OF VARIABILITY IN RICE TUNGRO VIRUS FROM SEVERAL ENDEMIC AREAS IN INDONESIA BY PCR-RFLP TECHNIQUE |
| title_sort | deteksi keragaman virus tungro dari beberapa daerah endemis di indonesia dengan teknik pcr rflp detection of variability in rice tungro virus from several endemic areas in indonesia by pcr rflp technique |
| topic | Jurnal i-lib UGM |
| work_keys_str_mv | AT perpustakaanugmilib deteksikeragamanvirustungrodaribeberapadaerahendemisdiindonesiadenganteknikpcrrflpdetectionofvariabilityinricetungrovirusfromseveralendemicareasinindonesiabypcrrflptechnique |