| Summary: | Kerapu macan (Epinephelus fuscoguttatus) merupakan salah satu diantara ikan kenipu yang berhasil dibudidayakan oleh petani petambak dan tingkat keberhasilan mencapai 40% pada penetasan kerapu industri skala rumah tangga di Bali. Virus viral nervous necrosis (VNN) merupakan penyebab kematian massal ikan kerapu, terutama larva dan juvenil. Pada penelitian ini, dikemba.vgkandan diaplikasikan uji imunositokimia streptavidin biotin (SB) untuk diagnosis dini VNN. Sampel (lendir) ikan kerapu yang diinfeksi virus VNN in vivo, diuji reverse transcriptase-polymerase chain reaction (RT-PCR).Lendir yang positifDNA VNN dengan RT-PCR,selanjutnya diuji SB. Hasil penelitian ini membuktikan, bahwa virus VNN dapat dideteksi pada lendir ikan kerapu macan tersebut yang terinfeksi virus VNN 24 jam sebelumnya. Disimpulkan, bahwa uji SB yang cepat dan akurat adalah tepat dan cocokuntuk diaplikasikan dalam rangka program rutin kontrol dan pencegahan VNN di Karantina Ikan Indonesia karena dapat dilakukan tanpa mematikan ikan, diterima secara ilmiah, hukum Dan internasional, dan bahkan tidak mencemari lingkungan hidup.
Kerapu macan (Epinephelusfuscoguttatus) is one of the kerapu fishes that has been successfully bred by the farmers. The survival rate of the fish reaches up to the 40% at the fish hatchery of the home industry of in Bali. , The disease in which may cause significant mortality in kerapu fish, especially for the larvae and juveniles is viral nervous necrosis (VNN). We therefore developed and applied the immunological diagnostic approach of immunocytochemistry technique of streptavidin biotin (SB) for early detection of VNN. Mucous samples of VNN infected-kerapu fish in vivo were firstly detectedby reverse transcriptase-polymerase chain reaction (RT- PCR) for the presence of DNAVNN. If it is RT-PCR-positive VNN, the mucous samples were then tested by SB. Results of the present study indicated that the VNN virus could be detected with SB technique within only 24 hours post infection. It was concluded that rapid and accurate SB technique is suitable and appropriate to be applied for routine control and prevention national program in the Fish Quarantine for Indon6siabecause no need of fish sacrification, and scientific, law and international accepted, and even no hazardous environmental contamination.
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